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HybridoMax Growth Supplement




+32 1658 9045


0032 (0)16 41 44 07

+32 1650 9045


Av. de l' Armée 68

B-1040 Brussels



tel 01 43 25 01 50

fax01 43 25 01 60

9, rue Lagrange

75005 Paris


tel 02 36 00 65 93

fax 02 36 00 65 94

20135 Milano


tel +32 1658 9045

fax +32 1650 9045


Tel 058 710 33 44

Fax 00 32 16 50 90 45

ul. Grunwaldzka 88A/2

81-771 Sopot


tel +81 78 386 0860

fax +81 78 306 0296


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0032 (0)16 41 44 07












Hybridoma Growth Supplement




As you may have heard is the FC-210001 ORIGEN Hybridoma Cloning factor no longer available.


GENTAUR is pleased to inform you we have a replacement for the FC210001 ORIGEN Hybridoma Cloning factor.

We have an alternative product which enables you to easily prepare your own Serum-Free Hybridoma Growth Medium.


The HybridoMax Hybridoma Growth Supplement replaces feeder layers or other conditioned media and growth factors used in hybridoma production. HybridoMax increases the yield of hybridomas surviving HAT selection, the cloning efficiency of B-cell hybridomas, and the number of antibody producing colonies.


The HybridoMax Hybridoma Growth Supplement is ready to use. Prepare the serum free Hybridoma growth medium by dissolving 1 bottle of HybridoMax (11 ml) into 1 Liter of D-MEM F-12 (Dulbecco's Modified Eagle's Medium F-12).


The prepared Serum-Free Hybridoma growth medium is a chemically defined culture medium supporting the serum-free growth of various hybridomas and 293 cells. The compounds of Hybridoma growth medium include water for injection, a modified DMEM/F12 base, HEPES buffer, known amounts of insulin, transferin, testosterone, sodium selenite, ethanolamine, a variety of saturated and unsaturated fatty acids, and stabilizing proteins. HybridoMax contains no IL-6 and no bovine serum albumine or other undefined protein mixtures.


HybridoMax Hybridoma Growth Supplement, 11 ml bottles (for 1 Liter of Hybridoma Growth Medium)

1 bottle of HybridoMax for 87 Euro. (19-HYB-01)

5 bottles of HybridoMax for 415 Euro. (19-HYB-05)

10 bottles of HybridoMax for 785 Euro. (19-HYB-10)


You can find the datasheet and the protocol here:



This protocol can be followed when using the TX-HYB supplemented Hybridoma Growth Medium in combination with a 1000ml cell Bioreactor.


We recommend to use the LM-D1223/500 DMEM – Ham’s F12 with L-Glutamine with 15mM HEPES together with the HybridoMax supplement to support optimal hybridoma development and achieve an optimal cell density. These are sold in bottles of 500 ml.

5 bottle of LM-D1223/500 for 59 Euro

10 bottles of LM-D1223/500 for 99 Euro.

20 bottles of LM-D1223/500 for 169 Euro.


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Copyright © 2002 GENTAUR Molecular Products
Last modified: 05/29/09