Anti Mouse Lgi4
Product Number 011311H12
Antibody specificity Anti-mouse Lgi4
Clone Number KT18
Isotype Rat IgG1
Antibody form Intact antibody
Concentration 1 mg/ml in PBS containing 0.1% sodium azide as a preservative
Cross-reactivity Not tested
Applications Western blotting, immunofluorescent staining. These are applications that have been tested. It does not mean that the antibody cannot be used for other applications. Figure 1. Lgi4 is expressed in Purkinje cells of the cerebellum when detected by KT18. Cryo-section was from adult mouse cerellum. Courtesy of Dr. Dies Meijer. Figure 2. Western blot of whole COS cell extracts using KT18. 1. Cells were transfected with HA-Oct6 as negative control; 2. Cells were transfected with Lgi4-myc expression cassette. Courtesy of Dr. Dies Meijer.
Suggested working concentrations Western blotting: starting from 1:2000, dilution range 1:500-1:5000; immunofluorescent staining: starting from 1:200, dilution range 1:100-1:1000.
Preparation Antibody is purified from culture supernatant using a protein G column
Store 4 °C for 12 months from date of despatch, -20 °C in aliquots for 2 years Safety information This product contains sodium azide which is hazardous
Figure 1. Lgi4 is expressed in Purkinje cells of the cerebellum when detected
by KT18. Cryo-section was from adult mouse cerellum. Courtesy of Dr. Dies
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