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Product sheet »

 

Product description

 

Background
Vitellogen (vg) in is a glucolipoprotein yolk precursor produced by all oviparous animals. This protein is female-specific and its expression is under hormonal control (estrogen). However, in the presence of Endocrine Disrupting Chemicals (EDCs), males can express the Vg gene in a dose dependent manner. The use of Vg gene expression in male fish can be used as a molecular marker of exposure to estrogenic EDCs.

Quantity:  200 ul
 
Immunogen: nNative vitellogenin (vg) purified from plasma of estradiol induced male of Senegalese sole (Solea senegalensis)

Type of antibody: polyclonal

Antibody format: serum, lyophilized

Tested applications: Western Blot (working dilution 1 : 5 000), ELISA (sole serum samples) titration starts with 1: 5000 

Reactivity:   The developed VTG ELISA, using these VTG and AbVTG, has been validated for Senegalese sole, sea bass (Dicentrarchus labrax) and seabream (Sparus aurata), which all gave parallel displacement curves in the assay. Probably, plasmas from several other fish species displace parallel and can also be used in the assay, although it has to be validated for each case.

If you are not certain that the antibody you are interested in will work in your specific application/species - please contact us!

ALL ANTIBODIES ARE FOR RESEARCH PURPOSE ONLY

 

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Last modified: 05/29/09