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CometAssay Control Cells

 

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CometAssay Control Cells

Trevigen’s CometAssay™ Control Cells are a set of cell preparations containing
different levels of DNA damage to be used with Trevigen’s CometAssay™ Kits. In a
typical comet assay, electrophoresis methods and differences in cell preparations create
a significant source of variation in comet tail parameters. Such variation sometimes
makes it difficult to compare results between laboratories, and even within the same
lab. To overcome this problem, Trevigen scientists developed a set of stable control
cell populations containing incremental levels of DNA damage for use when performing
the CometAssay™. These control cells, when electrophoresed in the CometAssay™,
consistently produce comets with predetermined percents of DNA in the tails. The
healthy control cell population (CC0) was treated with Etoposide under various
conditions to increase the amount of damage in populations CC1, CC2, and CC3,
respectively. These cryopreserved control cells are designed to act as standards to
compare alkaline electrophoresis methods between individual users and laboratories.

 

 

 

 

 

 

 

Damage Measurement for Control Cells

                       

 

Control Cells

n

Mean

SD

CCO

302

3.748

9.6864

CC1

300

23.737

14.6832

CC2

211

39.190

19.4772

CC3

235

57.122

24.4672

 

 

Features

Benefits

First available set of qualified control cells for use with the comet assay

Standardize and validate alkaline electrophoresis methods between individual users and laboratories

Includes four populations: one population of healthy cells and three populations with increasing amounts of DNA damage

Provides a range of DNA damage for comparison, which meets the needs of most laboratories.  Damaged populations show statistically distinct differences

Cells are cryopreserved

After initial thaw, aliquot and refreeze, the cells remain stable in liquid nitrogen for long term storage

Qualified for alkaline gel electrophoresis

Compatible with the electrophoresis method used by most laboratories

 

Ordering Information: CometAssay Controls
4256-010-CC ControlAssay™ Control Cells
 
Related Products: CometAssay Kits
4250-050-K  CometAssay™ Kit  4251-050-K 
CometAssay™ Silver Kit 4254-200-K   CometAssay™ 
Silver Staining Kit 4252-040-K  CometAssay™HT
Sample Kit 4253-096-K  CometAssay™ Kit 96 Wells
 

CometAssay Slides

4250-050-03

CometSlideTM (2-well slide) 

 25 slides 

4250-200-03

CometSlideTM (2-well slide)

  100 slides 

4250-500-01

CometAssayTM HT Slide (20 well slide)

 25 slides

4250-02K-01

CometAssayTM HT Slide (20 well slide)

100 slides

4253-960-03

96-Well CometSlideTM (96 well slide)

 10 slides

4253-02K-03

96-Well CometSlideTM (96 well slide)

 20 slides

4253-10K-03

96-Well CometSlideTM (96 well slide)

100 slides

 

 

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Last modified: 05/29/09