Catalogue Number: GENT 19 - INSTOP, 20ml, 200x solution, -20°C
Initiating a primary cell culture from a surgical tissue is often difficult because of contamination. It is therefore important to incubate the tissue in an appropriate solution containing an optimized mixture of antibiotics, each at a given concentration able to avoid infectious contamination without affecting cell viability. Furthermore, it is not always possible to initiaite primary cell culture immediately after surgical excision so the tissue needs to be stored for several hours until furhter processed.
InfectoSTOP (GENT 19 - INSTOP) is a ready-to-use solution containing an optimized mix of antibiotics against gram-negative and gram-positive bacteria, mycoplasma.
Simply dilute the necessary volume (enough to cover the tissue) of InfectoSTOP in PBS or in cell culture medium.
InfectoSTOP is intended for use in primary cell culture initiation or to extend cell viability in fresh surgical material of any tissue type. InfectoSTOP can also be used fore decontaminating established cell cultures.
The product should be handled under sterile conditions and is not intended for animal or human use.
Caution: If handled improperly, some components of this product may present a health hazard. Take appropriate precautions when handling this product, including the wearing of protective clothing and eyewear.
How to use InfetctoSTOP
To initiate primary cultures from fresh surgical material
Dilute InfectoSTOP 200x in PBS (4°C) and put the tissue in the freshly made solution. Rinse tissue immediately twice or more and then incubate 4 hours at 4°C with InfectoSTOP 100x in PBS. Prepare a fresh solution (150x in pBS), rinse once and incubate overnight (or at least one week without wiability loss, depending on the type of tissue) at 4°C.
Just before initiating primary cell culture, rinse once more with InfectoSTOP.
To eliminate contamination in anchorage-dependant cells
The above cell treatment can be repeated twice if necessary.
The dilution and incubation time of InfectoSTOP must be adjust to your own cell culture.
To eliminate contamination in anchorage-independant cells
Same procedure as above, but before rinsing and incubation, pellet the cells by brief centrifugation.
Storage and Stability
GLP techniques should be employed for the safe handling of this product.
This includes observing the following pratices:
Components: One bottle of 20mL.
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