Home Up Feedback Contents Search Sell your Prod. Meet us News           

 
Home • Up

Cellgraph

 

Home

GENTAUR

+32 1658 9045

or

0032 (0)16 41 44 07

+32 1650 9045

info@gentaur.com

Av. de l' Armée 68

B-1040 Brussels

BELGIUM

France

tel 01 43 25 01 50

fax01 43 25 01 60

9, rue Lagrange

75005 Paris

Italia

tel 02 36 00 65 93

fax 02 36 00 65 94

20135 Milano

Deutschland

tel +32 1658 9045

fax +32 1650 9045

Polska

Tel 058 710 33 44

Fax 00 32 16 50 90 45

ul. Grunwaldzka 88A/2

81-771 Sopot

日本

tel +81 78 386 0860

fax +81 78 306 0296

Minaatojimaminami-manchi

Chuo-ku, Kobe

065-0047

Österreich
+43720880899

Canada Montreal
+15149077481

Česká republika Praha
+420246019719

Danmark
+4569918806

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494

Magyarország Budapest
+3619980547

Ireland Dublin
+35316526556

Luxembourg
+35220880274

Nederland
+31208080893

Norge Oslo
+4721031366

Polska Warszawa
+48223988221

Sverige Stockholm
+46852503438

Schweiz Züri
+41435006251

US New York
+17185132983

Other Countries
0032 (0)16 41 44 07


 

 

 

 

 

Clonagen

Gentaur

Genprice

Bioxys

Labprice

Cellgraph

for Live Cell Imaging

to all researchers exploring the frontier of gene expression in living cells, tissues, organisms etc.

Cellgraph is a powerful instrument to capture the living cell images expressing a specific gene marked with bioluminescent proteins under long-term culture.

Cellgraph - Visualizing Dynamic Gene Expression Networks with Luciferase Markers

Living cell activities are regulated by the complex gene expression networks, which have been analyzed and illuminated with varieties of reporter gene methods using fluorescent proteins (GFP etc.) and/or bioluminescent proteins (firefly luciferase etc.).
Luciferases offer some advantages over fluorescent proteins, i.e. minimizing phototoxic effects causing damage to living cells, higher S/N without undesirable autofluorescence background emission or scatter generated by intracellular components.
Luciferase markers are also advantageous in long-term (up to several days) assay of living cells due to relatively low cytotoxicity of the substrate luciferin.
Sample chamber of Cellgraph Sample chamber with the lid open
 Cellgraph AB-3000B is an imaging system designed to detect extremely weak bioluminescent signals from a single cell. In combination with luciferase markers,  Cellgraph can visualize the cell dynamics - time dependent changes of transcriptional activities in individual cells and localization to inner cell components (organelles).

Advanced Features of  Cellgraph

 Cellgraph provides a variety of advanced features to serve living cell studies;
 

  • The ultrasensitive cooled and back-illuminated EMCCD (Electron Multiplying CCD) offers single photon sensitivity, certified with a patented procedure to measure total luminescence intensity as photon counts.
  • The incubation system enables long-term culture under controlled temperature and 5% carbon dioxide gas circulation.
  • The multicolor detection system is equipped for multi reporter assay with up to 2 different color filters, which enhances sensitivity to extra weak bioluminescence and gives an internal standard basis.
  • Versatile image capturing modes include; time-lapse capture at user-set interval, z-stepping mode to optimize Z-stage position (focal plane), switching mode to capture in a dark view and an illuminated view (a luminescent image and a bright field image) alternately.
  • Powerful tools for data analysis, editing, and presentation are also provided, i.e. grid measurement at view areas divided into the lattice of user-specified size, spot (ROI) measurement at user-selected areas of a chosen shape (ellipse, rectangle, polygon, or spline curve) and sizes, a movie maker converting time-lapse images into a movie file of AVI format.

Screenshot of spot measurement to select cells of interest (in ellipses) Luminescence intensity curves of cells selected in spot measurement (screenshot) A movie of luminescent cells on Windows Media Player

Applications of  Cellgraph AB-3000B

  •  Cellgraph has a huge potential to serve various fields of researches focussing gene expression in living cells, tissues, organisms;
    i.e. studies on chronobiology, development, cell differentiation, drug design, cancer, etc.
  • An original paper by Hoshino, H. et al, which refers to  Cellgraph, has been published on Nature Methods in August.
    Hoshino, H., Nakajima, Y., and Ohmiya, Y., Luciferase-YFP fusion tag with enhanced emission for single-cell luminescence imaging. Nature Methods. 4(8), 637-639 (2007) [NM] / [PubMed]

 also provides Kronos Dio, a luminometer for real-time reporter assay;

  • Kronos Dio is a Luminometer for real-time reporter assay with 8 dish incubator and multicolor measurement system. Kronos Dio provides a long-term multi culture monitoring tool which can be used in screening, examining different culture conditions on gene expression, etc.

Kronos Dio with the lid open screenshot of Kronos Dio software measuring 8 dishes

 

AB-2500 Kronos

 

More information: KronosDio.pdf

 

Cell graph

More information: Cellgraph.pdf

 

More information in general:

Real-time Monitoring of Gene Expression in Living Cell by Bioluminescent Detection System.pdf

Real-time Reporter Gene Assay.pdf

 

 

Send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2002 GENTAUR Molecular Products
Last modified: 05/29/09