Home Up Feedback Contents Search Sell your Prod. Meet us News           

 
Home • Up

CultreCoat Collagen I

 

Home

GENTAUR

+32 1658 9045

or

0032 (0)16 41 44 07

+32 1650 9045

info@gentaur.com

Av. de l' Armée 68

B-1040 Brussels

BELGIUM

France

tel 01 43 25 01 50

fax01 43 25 01 60

9, rue Lagrange

75005 Paris

Italia

tel 02 36 00 65 93

fax 02 36 00 65 94

20135 Milano

Deutschland

tel +32 1658 9045

fax +32 1650 9045

Polska

Tel 058 710 33 44

Fax 00 32 16 50 90 45

ul. Grunwaldzka 88A/2

81-771 Sopot

日本

tel +81 78 386 0860

fax +81 78 306 0296

Minaatojimaminami-manchi

Chuo-ku, Kobe

065-0047

Österreich
+43720880899

Canada Montreal
+15149077481

Česká republika Praha
+420246019719

Danmark
+4569918806

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494

Magyarország Budapest
+3619980547

Ireland Dublin
+35316526556

Luxembourg
+35220880274

Nederland
+31208080893

Norge Oslo
+4721031366

Polska Warszawa
+48223988221

Sverige Stockholm
+46852503438

Schweiz Züri
+41435006251

US New York
+17185132983

Other Countries
0032 (0)16 41 44 07


 

 

 

 

 

Clonagen

Gentaur

Genprice

Bioxys

Labprice

CultreCoat® Collagen I 96 Well Cell Adhesion Assay
 

CultreCoat® Collagen I Cell Adhesion Assay provides a simple, standardized, high throughput format for assessing factors that influence cell-matrix interactions. The black stripwell plate minimizes background, providing greater sensitivity, and affords flexibility for the number of samples assessed. The stripwell feature also allows multiple experiments to be conducted simultaneously using the same kit. Calcein labeling allows direct comparisons between the number of cells that are loaded and the number that adhere, providing a loading control and the percent cell adhesion based on the final fluorescence assessed for each well. Controls are provided for determining background and non-specific binding.

 
Applications: Assess factors that influence cell-matrix interactions.
 
Components: Part # Component Size
  3492-096-P Collagen I 96 Well CA Plate 1 Plate
  3490-096-01 2X CA Assay Buffer 100 ml
  3490-096-02 10X CA Blocking Agent 3 ml
  4892-010-01 Calcein AM 50 µg
 
Storage: Components are stored at -20ºC, 4ºC, and at room temperature.
 
References: 1. Dike, L.E. and D.E. Ingber, Integrin-dependent induction of early growth response genes in capillary endothelial cells. J Cell Sci, 1996. 109 (Pt 12): p. 2855-63.

2. Zhu, X., et al., Adhesion-dependent cell cycle progression linked to the expression of cyclin D1, activation of cyclin E-cdk2, and phosphorylation of the retinoblastoma protein. J Cell Biol, 1996. 133(2): p. 391-403.

3. Takeuchi, Y., et al., Differentiation and transforming growth factor-beta receptor down-regulation by collagen-alpha2beta1 integrin interaction is mediated by focal adhesion kinase and its downstream signals in murine osteoblastic cells. J Biol Chem, 1997. 272(46): p. 29309-16.



 

 
Download Protocol
data icon
 

Related Products

CultreCoat® Laminin I 96 Well Cell Adhesion Assay

CultreCoat® BME 96 Well Adhesion Assay

CultreCoat® Vitronectin 96 Well Cell Adhesion Assay

CultreCoat® Collagen IV 96 Well Cell Adhesion Assay

CultreCoat® Adhesion Protein Array 96 Well Cell Adhesion Assay

CultreCoat® Fibronectin 96 Well Cell Adhesion Assay

 

Send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2002 GENTAUR Molecular Products
Last modified: 05/29/09