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Diagnostics of Autoimmune Disease

 

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DIAGNOSTICS OF AUTOIMMUNE DISEASES

K131            K132            K133

 


K131 Thyroperoxidase autoantobodies (anti-TPO) EIA

Anti-TPO antibodies (formerly - thyroid microsomal antibodies) are directed against a target protein -
thyroid peroxidase (TPO) - located in the smooth endoplasmic reticulum of thyroid cells. The presence
of anti-TPO antibodies in serum is associated with thyroid autoimmune diseases (Graves’ disease and
Hashimoto’s thyroiditis). Anti-TPO antibodies mostly belong to the IgG class.
Low to moderate levels of serum anti-TPO antibodies can be found in some other autoimmune
pathology (eg systemic lupus erythematosus or Sjogren syndrom) and, rarely, in apparently healthy
subjects (especially elderly women). Anti-TPO antibodies are more sensitive in diagnosis of thyroid
autoimmune diseases than anti-thyroglobulin (anti-TG) antibodies. However, in some cases anti-TG
positive sera may be negative for anti-TPO. Therefore, combined determination of both types of
anti-thyroid antibodies (anti-TPO + anti-TG) provides a more sensitive laboratory diagnostic tool
for thyroid autoimmunity.
 


Sample type: serum, plasma                         Sensitivity: 5 IU/ml                                   Control sample: 1
Sample predilution: 1:101                             Incubation: 30’/30’/15’, RT                         Shelf life: 12 months
Sample volume: 100 μl                                 Calibrators: 5 (0-1000 IU/ml)
 

 

K132 Thyroglobulin autoantobodies (anti-TG) EIA

Thyroglobulin (TG) is a well known target for autoantibodies occurring in thyroid autoimmunity
(Graves’ disease and Hashimoto’s thyroiditis). Anti-TG antibodies mostly belong to the IgG class.
Low to moderate levels of anti-TG antibodies can be found in sera of other autoimmune patients (eg
systemic lupus erythematosus or Sjogren syndrom). In some cases anti-TG positive sera may show
negativity for other type of anti-thyroid antibodies - anti-TPO. Therefore, combined determination of
both types of anti-thyroid antibodies (anti-TPO + anti-TG) provides most sensitive laboratory diagnostic
tool for thyroid autoimmunity. Separately from autoimmunity, anti-TG antibodies may develop in
patients suffering from thyroid cancer. High level of anti-TG in such patients may interfere with correct
determination of serum thyroglobulin which serves as tumor marker for therapy control in this group
of patients.

 

Sample type: serum, plasma                         Sensitivity: 10 IU/ml                                 Control sample: 1
Sample predilution: 1:101                             Incubation: 30’/30’/15’, RT                         Shelf life: 12 months
Sample volume: 100 μl                                 Calibrators: 5 (0-3000 IU/ml)
 

 

K133 Myeloperoxidase autoantibodies (anti-MPO, p-ANCA) EIA

Antibodies to myeloperoxidase (AMPO) are one of the two main members of anti-neutrophilic-cytoplasm
antibodies (ANCA). ANCA are directed towards cytoplasmic components of neutrophilic granulocytes
and monocytes. The classical method for determination of ANCA is immunofluorescense. Indirect
immunofluorescense (IF) reveal two main patterns: perinuclear (p-ANCA) type and cytoplasmatic (c-ANCA)
type. The main antigen for p-ANCA is myeloperoxidase (MPO) while for c-ANCA the main antigen is
proteinase 3 (PR3).
A similar fluorescent p-ANCA pattern may accompany the presence of autoantibodies to other antigens
–e.g., elastase, catepsin G, lactoferrin and lysozyme. Besides, indistinguishable from p-ANCA pattern
may be caused by different granulocyte specific antinuclear antibodies (GS-ANA) with atypical pattern.
Therefore, an exact interpretation of IF patterns is difficult, and every positive p-ANCA pattern should
be confirmed by EIA methods using purified antigens.
AMPO are mostly found in patients with eosinophilic vasculitides, including Takayasu syndrom, Churg-
Strauss syndrom and periarteriitis nodosa (PAN). Combined positivity with anti-PR3 is observed
in ca.70% of the patients with microscopic polyangiitis.

 

Sample type: serum, plasma                         Incubation: 30’/30’/15’, RT                          Control sample: 1
Sample volume: 100 μl                                 Calibrators: 6 (0-100 U/ml)                         Shelf life: 12 months
Sensitivity: 3 U/ml

 

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Last modified: 05/29/09