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Poly (ADP-ribose) Polymerase (PARP)

Anti-PAR Polymer Monoclonal Antibody Now more sensitive!
Anti-PAR Polymer Polyclonal Antibody
Anti-PARP Monoclonal Antibody
Universal Colorimetric PARP Assay Kit
Universal Chemiluminescent PARP Assay Kit
PARP Activity Assay Kit
Biotinylated NAD
FITC-NAD
PARP Proteins
PARP Inhibitors
 

Anti-PAR Polymer Monoclonal Antibody Now more sensitive!

Anti-PAR Polymer Monoclonal Antibody
 
Poly (ADP-ribose) polymerase (PARP) converts NAD into nicotinamide and polymers of ADP-ribose at glutamic acid residues of nuclear proteins. The majority of polymer chains are linked to PARP itself through automodification. Poly(ADP-ribosyl)ation of subsets of nuclear proteins plays an important role in cell DNA repair, proliferation, replication, recombination, and apoptosis.
 
Immunogen: The antibody was raised against purified PAR polymer chains of 2 to 50 units long.
   
Specificity: The antibody is specific for poly (ADP-ribose) polymers and does not recognize structurally related RNA, DNA, ADP-ribose monomers, NAD or other nucleic acid monomers.
   
Subtype: IgG3a
   
Preparation: The antibody is purified from ascites fluid and stored in unpreserved PBS buffer. The antibodys range of use is at dilutions of 1:500 to 1:2,000.
   
Applications:
   Western blotting
   ELISA
   Immunohistochemistry
   Immunocytochemistry
   
Storage: Freeze in working aliquots at -20 C or -80 C in a manual defrost freezer to avoid repeated freeze-thawing.
   
Control: Bacterially expressed human PARP-treated protein (Cat# 4500-50-P) included.

Products

4335-MC-100 Anti-PAR Polymer Monoclonal Antibody, 100 l
4500-50-P PARP Treated Protein Control for Western Blot, 50 l

 

Anti-PAR Polymer Polyclonal Antibody

Anti-PAR Polymer Polyclonal Antibody
 
Poly (ADP-ribose) polymerase (PARP) converts NAD into nicotinamide and polymers of ADP-ribose at glutamic acid residues of nuclear proteins. The majority of polymer chains are linked to PARP itself through automodification. Poly(ADP-ribosyl)ation of subsets of nuclear proteins plays an important role in cell DNA repair, proliferation, replication, recombination, and apoptosis.
 
 
Immunogen: The antibody was raised against purified PAR polymer chains of 10 to 50 units long.
   
Specificity: The antibody recognizes free poly (ADP-ribose) and poly-ribosylated proteins.
   
Preparation: The antibody is prepared in rabbit and supplied as purified IgG fraction in 1X PBS. A starting antibody dilution of 1:1000 is recommended.
   
Applications: Western blotting
   
Storage: Freeze at -20C or -80C in a manual defrost freezer to avoid repeated freeze-thawing.

Products

4336-BPC-100 Anti-PAR Polymer Polyclonal Antibody, 100 l


Anti-PARP Monoclonal Antibody

Anti-PARP Monoclonal Antibody (clone C2-10)
 
The anti-poly (ADP-ribose) polymerase (PARP) mouse monoclonal antibody may be used for the analysis of PARP protein levels and the cleavage of PARP in apoptotic cells.
 
Immunogen: The antibody was raised against full length calf thymus poly (ADP-ribose) polymerase.
   
Specificity: It recognizes both the full length protein (116 kDa) as well as an 85 kDa cleavage fragment associated with apoptosis. The recognized epitope is within the C-terminal part of the DNA binding domain of PARP. The antibody cross-reacts with human, monkey, hamster, rat and mouse PARP, but does not recognize chicken PARP.
   
Subtype: IgG1
   
Preparation: The antibody is purified from mouse ascites and is provided in PBS containing 0.2% BSA and 0.05% sodium azide.
   
Applications: Western blotting
Immunohistochemistry
   
Storage: Freeze in working aliquots at -20C in a manual defrost freezer to avoid repeated freeze-thawing.
   
Control: HSA PARP Enzyme available separately.

Products

4338-MC-50 Anti-PARP Monoclonal Antibody (clone C2-10), 50 g

 

Universal Colorimetric PARP Assay Kit

Universal Colorimetric PARP Assay Kit
 
Poly ADP-ribosylation of nuclear proteins is a post-translational event that occurs in response to DNA damage. Poly (ADP-ribose) polymerase (PARP) catalyzes the NAD-dependent addition of poly (ADP-ribose) to adjacent nuclear proteins. PARP plays an important role in DNA repair but can also lead to apoptosis by depleting the cellular NAD pool. Experimental models have shown that PARP inhibition prevents tissue damage in animal models of myocardial and neuronal ischemia, diabetes, septic shock, and vascular stroke. Recent data implicate a synergistic function of Ku80 and PARP-1 in minimizing chromosome aberrations and cancer development. Trevigen's Universal 96-well PARP Assay Kit measures the incorporation of biotinylated Poly (ADP-ribose) onto histone proteins in a 96-well plate format. This assay is ideal for the screening of PARP inhibitors and for measuring the activity of PARP in cell and tissue extracts.
 
Features:
   Colorimetric, non-radioactive format
   Higher throughput 96 test size
   Sensitivity down to 0.01 units of PARP per well
   
Applications:
   Identify inhibitors and activators of PARP activity
   Measure caspase inactivation of PARP
   Quantitate levels of DNA damage in cells caused by a variety of genotoxic agents
   Measuring activity of PARP in cell and tissue extracts
   
Components: Catalog # Component Size
  4668-050-01
 
HSA PARP Enzyme 50 Units
  4671-096-02 *20X PARP Buffer 500 l
  4671-096-03 *10X PARP Cocktail** 300 l
  4667-50-07 *Histone Solution (A) 700 l
  4667-50-03 *3-Aminobenzamide
 
60 l
  4672-096-P Histone-Coated Plate (B) 1 plate
  4671-096-04 10X Strep-Diluent
 
2 l
  4800-30-06 Strep-HRP 30 l
  4822-96-08 TACS-Sapphire 10 l
 
   
Storage: Components are stored at -80C and 4C.
   
References:

Products

4671-096-K Universal PARP Assay Kit w/Histone Reagent, 96 samples
4672-096-K Universal PARP Assay Kit w/Histone Coated Plate, 96 samples

 

Universal Chemiluminescent PARP Assay Kit

Universal Chemiluminescent PARP Assay Kit
 
Poly ADP-ribosylation of nuclear proteins is a post-translational event that occurs in response to DNA damage. Poly (ADP-ribose) polymerase (PARP) catalyzes the NAD-dependent addition of poly (ADP-ribose) to adjacent nuclear proteins. PARP plays an important role in DNA repair but can also lead to apoptosis by depleting the cellular NAD pool. Experimental models have shown that PARP inhibition prevents tissue damage in animal models of myocardial and neuronal ischemia, diabetes, septic shock, and vascular stroke. Recent data implicate a synergistic function of Ku80 and PARP-1 in minimizing chromosome aberrations and cancer development. Trevigen's Universal 96-well PARP Assay Kit measures the incorporation of biotinylated Poly (ADP-ribose) onto histone proteins in a 96-well plate format. This assay is ideal for the screening of PARP inhibitors and for measuring the activity of PARP in cell and tissue extracts.
 
Features:
   Chemiluminescent, non-radioactive format
   Higher throughput 96 test size
   sensitivity down to 0.0025 units of PARP per well
   
Applications:
   Identify inhibitors and activators of PARP activity
   Measure caspase inactivation of PARP
   Quantitate levels of DNA damage in cells caused by a variety of genotoxic agents
   Measuring activity of PARP in cell and tissue extracts
   
Components: Catalog # Component Size
  4668-50-01
 
PARP-HSA 50 l
  4671-096-02 *20X PARP Buffer 500 l
  4671-096-03 *10X PARP Cocktail** 300 l
  4671-096-05 5X Coating Buffer (A) 1.4 ml
  4667-50-07 *Histone Solution (A) 700 l
  4667-096-P *Histone-Coated Strip wells (B) 96
  4667-50-03 *3-Aminobenzamide 60 l
  4671-096-04 10X Strep-Diluent 2 ml
  4800-30-06 Strep-HRP 30 l
  4675-096-01 PeroxyGlow A 6 ml
  4800-30-06 PeroxyGlow B 6 ml
  4671-096-06 *10X Activated DNA 300 l
 
   
Storage: Components are stored at -80C and 4C.
   
References:

Products

4675-096-K Universal Chemiluminescent PARP Assay Kit with Histone Reagent (96 samples)
4676-096-K Universal Chemiluminescent PARP Assay Kit with Histone Coated Plate (96 samples)


PARP Activity Assay Kit

PARP Activity Assay Kit
 
The functional activity of PARP can be determined using Trevigens in vitro PARP Activity Assay kit. PARP activity is measured by determining the level of incorporation of radiolabeled NAD in a TCA precipitable polymer. The PARP enzyme is activated in the presence of damaged DNA. The level of PARP activation is directly proportional to the amount of damage encountered within the cell, and therefore an effective measure of total DNA damage within the sample. DNA damage can be assessed in crude extracts of cells or tissues.

The addition of activated DNA (sheared genomic DNA) to cell lysates allows for the investigation of maximal PARP activity. In apoptotic cells, the inactivation of PARP by proteolytic cleavage can be assayed by measuring decreases in PARP activity following induction of apoptosis.

 
Features:
   Rapid assay requires approximately 10 minutes
   Sensitive
   Contains a positive control of PARP enzyme
   Includes all reagents except for radiolabeled NAD
   Uses crude extracts from cells or tissues
   
Applications:
   Identify inhibitors and activators of PARP activity
   Measure caspase inactivation of PARP
   Quantitate levels of DNA damage in cells caused by a variety of genotoxic agents
   
Components: Catalog # Component Size
  4667-50-01
 
PARP Enzyme 50 l
  4667-50-02 10X PARP Buffer 700 l
  4667-50-03 3-Aminobenzamide 60 l
  4667-50-04 NAD* 700 l
  4667-50-05 Distilled Water 3.75 ml
  4667-50-06 Activated DNA
 
500 l
  4667-50-07 Histones 700 l
  * User must supply isotopic NAD
   
Items Not Included: 32P- or 3H-NAD
10% and 20% trichloroacetic acid
   
Storage: Store components at -20C.
   
References: To determine PARP activity using a non-isotopic alternative to radiolabeled NAD:
4667-50-K PARP Assay Kit, 50 Samples


Biotinylated NAD

Biotinylated NAD
 
Trevigens biotinylated NAD (6-biotin-17-nicotinamide-adenine-dinucleotide) provides a convenient non-isotopic alternative to radiolabeled NAD for studies requiring this substrate. A number of proteins, including poly (ADP-ribose) polymerase (PARP), use NAD as a substrate for their function. The PARP enzyme catalyzes the polymerization of ADP-ribose onto a number of nuclear proteins. Biotinylated NAD allows an indirect measure of PARP activity when biotin incorporation is detected using a conjugated-streptavidin detection system.
 
Applications:
   Activity measurements for NAD requiring enzymes
   Assays to identify PARP inhibitors
   
Preparation: Provided as a buffered solution containing 250 M 6-biotin-17-nicotinamide-adenine-dinucleotide, sodium salt.
   
Molecular Weight: 1179 g/mol
   
Extinction Coefficient: 22,000 at 265 nm
 
   
Purity: >95% by HPLC
   
Storage: Store at -80C in working aliquots to avoid repeated freeze-thawing.

Products

4670-500-01 Biotinylated NAD, 500 l
4670-500-05 Biotinylated NAD, 5 x 500 l
4670-500-10 Biotinylated NAD, 10 x 500 l


FITC-NAD

FITC-NAD
 
FITC-NADF NAD for use with enzymes requiring NAD as a substrate or cofactor. A number of proteins, including poly (ADP-ribose) polymerase (PARP), and the SIR2 family of NAD(+)-dependent histone/protein deacetylases, use NAD as a substrate for their function. FITC conjugated NAD permits the direct measurement of PARP and other NAD-dependent enzymes by fluorescence microscopy or by incorporation of fluorescent-labeled poly (ADP-ribose) or O-acety-ADP ribose onto histones attached to a 96 well plate.
 
Applications:
   Activity measurements for NAD requiring enzymes
   Assays to identify inhibitors of NAD-requiring enzymes
   Cell Permeabilization reagent, 4674-250-01, required for in-cell assays
   
Physical State: Provided in solution at a concentration of 250 m in deionized water. There is a 1:1 stoichiometry for incorporation; one FITC label for each NAD molecule.
   
Extinction Coefficient: 21,000 at 265nm
   
Purity: 95% by HPLC
 
   
Storage: Store at -80C in working aliquots to avoid repeated freeze-thawing.

Products

4673-500-01 FITC-NAD, 250 l
4673-500-05 FITC-NAD, 5 x 250 l
4673-500-10 FITC-NAD, 10 x 250 l


PARP Proteins

Human PARP Enzyme
 
Trevigen now offers two grades of poly (ADP-ribose) polymerase: a standard PARP, and a new high specific activity (HSA) enzyme. The specific activity serves as a measure of enzyme purity, with the standard enzyme being purified to approximately 50% purity, and the high specific activity enzyme to greater than 95%. The standard PARP enzyme is useful as a positive control for both Western blot analysis of ribosylated proteins and the PARP Activity Assay (Cat# 4667-50-K). For more detailed studies or for drug discovery applications, the HSA PARP is recommended. The catalytic domain of PARP is also available.
 
Unit Definition: One unit is the amount of enzyme required to incorporate 100 pmol of poly(ADP) from NAD into acid-insoluble form in 1 minute at 22C.
   
Source: Purified from E. coli containing a recombinant plasmid encoding the full-length human PARP gene.
   
Applications:
   Identification of inhibitors and activators of PARP activity
   Quantitation of DNA damage
   Investigation of PARP inactivation during apoptosis
   
Storage: Freeze in working aliquots at -80 C to avoid repeated freeze-thawing.

Products

4667-50-01 PARP Enzyme, 100 Units
4667-250-01 PARP Enzyme, 500 Units
4668-100-01 PARP Enzyme, High Specific Activity, 100 Units
4668-500-01 PARP Enzyme, High Specific Activity, 500 Units
4668-2K-01 PARP Enzyme, High Specific Activity, 2000 units
4669-100-01 Chicken PARP Catalytic Domain, 100g
* 4669-100-02 Human PARP Catalytic Domain, 100g


PARP Inhibitors
 

PARP Inhibitors
 
DNA bullet 3-Aminobenzamide
3-aminobenzamide is the PARP inhibitor provided with Trevigen's PARP Inhibition Assay Kit (Cat# 4669-96-K) at 200 mM.
 
DNA bullet 4-Amino-1,8-naphthalimide
4-Amino-1,8-naphthalimide is a potent inhibitor of poly(ADP-ribose) polymer (PARP) and reduces ischemia-reperfusion injury in the heart and skeletal muscle. It is 1000-fold more potent that 3-aminobenzamide and exhibits mixed-type inhibition with respect to the sub-strate, NAD+, at micromolar concentrations. This inhibitor, which has been analyzed at a concentration of 20 M, is provided at a convenient concentration for subsequent serial dilution and use with Trevigen's PARP Inhibition Assay Kit (Cat# 4669-96-K).
 
DNA bullet 6(5H)-Phenanthridinone
6(5H)-Phenanthridinone strongly inhibits poly(ADP-ribose) polymerase (PARP) and displays immunosuppressive activity. It is a mixed-type inhibitor that acts on both the enzyme and enzyme-NAD+ complex at site(s) distinct from the NAD+-binding site to attenuate the fall in NAD and ATP and, consequently, improve cell survival. It also inhibits concanavalin A-induced lymphocyte proliferation at micromolar concentrations. This inhibitor, which has been analyzed in the range of 0.18-0.39 M, is provided at a convenient concentration for subsequent serial dilution and use with Trevigen's PARP Inhibition Assay Kit (Cat# 4669-96-K).
 
DNA bullet Benzamide
Benzamide is the most potent poly(ADP-ribose) polymerase (PARP) inhibitor in the family of benzamides. It acts as a neuroprotectant since it inhibits PARP, an enzyme activated by nitric oxide. Benzamide is twice as active than its commonly used counterpart, 3-aminobenzamide in delaying or suppressing PARP activation. It is able to prevent nuclear fragmentation and apoptotic-body formation without affecting DNA fragmentation during apoptosis. This PARP inhibitor, which has been analyzed in the range of 100 to 500 M, is provided at a convenient concentration for subsequent serial dilution and use with Trevigen's PARP Inhibition Assay Kit (Cat# 4669-96-K).

Products

4667-50-03 3-Aminobenzamide, 60 l
4667-50-09 4-Amino-1,8-naphthalimide 100 l
4667-50-10 6(5H)-Phenanthridinone, 100 l
4667-50-11 Benzamide, 100 l
 

 

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Last modified: 05/29/09