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GENTAUR
 +32 1658 9045
or
0032 (0)16 41 44 07
 +32 1650 9045
[email protected]
Av. de l' Armée 68
B-1040 Brussels
BELGIUM
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0032 (0)16 41 44 07
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Poly (ADP-ribose) Polymerase
(PARP)
Anti-PAR Polymer Monoclonal Antibody Now more sensitive!
Anti-PAR Polymer
Polyclonal Antibody
Anti-PARP Monoclonal
Antibody
Universal
Colorimetric PARP Assay Kit
Universal
Chemiluminescent PARP Assay Kit
PARP Activity Assay Kit
Biotinylated NAD
FITC-NAD
PARP Proteins
PARP Inhibitors
|
Poly (ADP-ribose) polymerase (PARP) converts NAD into
nicotinamide and polymers of ADP-ribose at glutamic acid
residues of nuclear proteins. The majority of polymer chains are
linked to PARP itself through automodification.
Poly(ADP-ribosyl)ation of subsets of nuclear proteins plays an
important role in cell DNA repair, proliferation, replication,
recombination, and apoptosis.
|
| |
| The antibody was raised against
purified PAR polymer chains of 2 to 50 units long. |
| |
| The antibody is specific for poly
(ADP-ribose) polymers and does not recognize structurally
related RNA, DNA, ADP-ribose monomers, NAD or other nucleic acid
monomers. |
| |
| IgG3a |
| |
| The antibody is purified from
ascites fluid and stored in unpreserved PBS buffer. The
antibody’s range of use is at dilutions of 1:500 to 1:2,000. |
| |
| • |
Western blotting
 |
| • |
ELISA
|
| • |
Immunohistochemistry
|
| • |
Immunocytochemistry |
|
| |
| Freeze in working aliquots at -20 C
or -80 C in a manual defrost freezer to avoid repeated
freeze-thawing. |
| |
| Bacterially expressed human
PARP-treated protein (Cat# 4500-50-P) included. |
Products
|
4335-MC-100 |
Anti-PAR Polymer Monoclonal Antibody, 100 µl |
|
4500-50-P |
PARP Treated Protein Control for Western Blot, 50
µl |
Poly (ADP-ribose) polymerase (PARP) converts NAD into
nicotinamide and polymers of ADP-ribose at glutamic acid
residues of nuclear proteins. The majority of polymer chains are
linked to PARP itself through automodification.
Poly(ADP-ribosyl)ation of subsets of nuclear proteins plays an
important role in cell DNA repair, proliferation, replication,
recombination, and apoptosis.
|
| |
| The antibody was raised against
purified PAR polymer chains of 10 to 50 units long. |
| |
| The antibody recognizes free poly
(ADP-ribose) and poly-ribosylated proteins. |
| |
| The antibody is prepared in rabbit
and supplied as purified IgG fraction in 1X PBS. A starting
antibody dilution of 1:1000 is recommended. |
| |
| • Western blotting |
| |
| Freeze at -20°C or -80°C in a
manual defrost freezer to avoid repeated freeze-thawing. |
Products
|
4336-BPC-100 |
Anti-PAR Polymer Polyclonal Antibody, 100 µl |
|
The anti-poly (ADP-ribose) polymerase (PARP) mouse monoclonal
antibody may be used for the analysis of PARP protein levels and
the cleavage of PARP in apoptotic cells. |
| |
| The antibody was raised against
full length calf thymus poly (ADP-ribose) polymerase. |
| |
| It recognizes both the full length
protein (116 kDa) as well as an 85 kDa cleavage fragment
associated with apoptosis. The recognized epitope is within the
C-terminal part of the DNA binding domain of PARP. The antibody
cross-reacts with human, monkey, hamster, rat and mouse PARP,
but does not recognize chicken PARP. |
| |
| IgG1 |
| |
| The antibody is purified from mouse
ascites and is provided in PBS containing 0.2% BSA and 0.05%
sodium azide. |
| |
• Western blotting
• Immunohistochemistry |
| |
| Freeze in working aliquots at -20°C
in a manual defrost freezer to avoid repeated freeze-thawing. |
| |
|
HSA PARP
Enzyme available separately. |
Products
|
4338-MC-50 |
Anti-PARP Monoclonal Antibody (clone C2-10), 50
µg |
|
Poly ADP-ribosylation of nuclear proteins is a
post-translational event that occurs in response to DNA damage.
Poly (ADP-ribose) polymerase (PARP) catalyzes the NAD-dependent
addition of poly (ADP-ribose) to adjacent nuclear proteins. PARP
plays an important role in DNA repair but can also lead to
apoptosis by depleting the cellular NAD pool. Experimental
models have shown that PARP inhibition prevents tissue damage in
animal models of myocardial and neuronal ischemia, diabetes,
septic shock, and vascular stroke. Recent data implicate a
synergistic function of Ku80 and PARP-1 in minimizing chromosome
aberrations and cancer development. Trevigen's Universal 96-well
PARP Assay Kit measures the incorporation of biotinylated Poly
(ADP-ribose) onto histone proteins in a 96-well plate format.
This assay is ideal for the screening of PARP inhibitors and for
measuring the activity of PARP in cell and tissue extracts.
|
| |
| • |
Colorimetric,
non-radioactive format
|
| • |
Higher throughput 96 test
size
|
| • |
Sensitivity down to 0.01
units of PARP per well |
|
| |
| • |
Identify inhibitors and
activators of PARP activity
|
| • |
Measure caspase
inactivation of PARP
|
| • |
Quantitate levels of DNA
damage in cells caused by a variety of genotoxic agents
|
| • |
Measuring activity of PARP
in cell and tissue extracts |
|
| |
| Catalog # |
Component |
Size |
4668-050-01
|
HSA
PARP Enzyme |
50
Units |
|
4671-096-02 |
*20X PARP Buffer |
500
µl |
|
4671-096-03 |
*10X PARP Cocktail** |
300
µl |
|
4667-50-07 |
*Histone Solution (A) |
700
µl |
|
4667-50-03 |
*3-Aminobenzamide
|
60
µl |
|
4672-096-P |
Histone-Coated Plate (B) |
1
plate |
|
4671-096-04 |
10X
Strep-Diluent
|
2 µl
|
|
4800-30-06 |
Strep-HRP |
30
µl |
|
4822-96-08 |
TACS-Sapphire™ |
10
µl |
| |
| Components are stored at -80°C and
4°C. |
| |
Products
|
4671-096-K |
Universal PARP Assay Kit w/Histone Reagent, 96
samples |
|
4672-096-K |
Universal PARP Assay Kit w/Histone Coated Plate,
96 samples |
|
Poly ADP-ribosylation of nuclear proteins is a
post-translational event that occurs in response to DNA damage.
Poly (ADP-ribose) polymerase (PARP) catalyzes the NAD-dependent
addition of poly (ADP-ribose) to adjacent nuclear proteins. PARP
plays an important role in DNA repair but can also lead to
apoptosis by depleting the cellular NAD pool. Experimental
models have shown that PARP inhibition prevents tissue damage in
animal models of myocardial and neuronal ischemia, diabetes,
septic shock, and vascular stroke. Recent data implicate a
synergistic function of Ku80 and PARP-1 in minimizing chromosome
aberrations and cancer development. Trevigen's Universal 96-well
PARP Assay Kit measures the incorporation of biotinylated Poly
(ADP-ribose) onto histone proteins in a 96-well plate format.
This assay is ideal for the screening of PARP inhibitors and for
measuring the activity of PARP in cell and tissue extracts.
|
| |
| • |
Chemiluminescent,
non-radioactive format
|
| • |
Higher throughput 96 test
size
|
| • |
sensitivity down to 0.0025
units of PARP per well |
|
| |
| • |
Identify inhibitors and
activators of PARP activity
|
| • |
Measure caspase
inactivation of PARP
|
| • |
Quantitate levels of DNA
damage in cells caused by a variety of genotoxic agents
|
| • |
Measuring activity of PARP
in cell and tissue extracts |
|
| |
| Catalog # |
Component |
Size |
4668-50-01
|
PARP-HSA |
50
µl |
|
4671-096-02 |
*20X PARP Buffer |
500
µl |
|
4671-096-03 |
*10X PARP Cocktail** |
300
µl |
|
4671-096-05 |
5X
Coating Buffer (A) |
1.4
ml |
|
4667-50-07 |
*Histone Solution (A) |
700
µl |
|
4667-096-P |
*Histone-Coated Strip wells (B) |
96 |
|
4667-50-03 |
*3-Aminobenzamide |
60
µl |
|
4671-096-04 |
10X Strep-Diluent |
2
ml |
|
4800-30-06 |
Strep-HRP |
30
µl |
|
4675-096-01 |
PeroxyGlow™
A |
6
ml |
|
4800-30-06 |
PeroxyGlow™
B |
6 ml
|
|
4671-096-06 |
*10X Activated DNA |
300
µl |
| |
| Components are stored at -80°C and
4°C. |
| |
Products
|
4675-096-K |
Universal Chemiluminescent PARP Assay Kit with
Histone Reagent (96 samples) |
|
4676-096-K |
Universal Chemiluminescent PARP Assay Kit with
Histone Coated Plate (96 samples) |
|
4667-50-K |
PARP Assay Kit, 50 Samples |
|
Trevigen’s biotinylated NAD
(6-biotin-17-nicotinamide-adenine-dinucleotide) provides a
convenient non-isotopic alternative to radiolabeled NAD for
studies requiring this substrate. A number of proteins,
including poly (ADP-ribose) polymerase (PARP), use NAD as a
substrate for their function. The PARP enzyme catalyzes the
polymerization of ADP-ribose onto a number of nuclear proteins.
Biotinylated NAD allows an indirect measure of PARP activity
when biotin incorporation is detected using a
conjugated-streptavidin detection system. |
| |
| • |
Activity measurements for
NAD requiring enzymes
|
| • |
Assays to identify PARP
inhibitors |
|
| |
| Provided as a buffered solution
containing 250 µM 6-biotin-17-nicotinamide-adenine-dinucleotide,
sodium salt. |
| |
| 1179 g/mol |
| |
22,000 at 265 nm
|
| |
| >95% by HPLC |
| |
| Store at -80°C in working aliquots
to avoid repeated freeze-thawing. |
Products
|
4670-500-01 |
Biotinylated NAD, 500 µl |
|
4670-500-05 |
Biotinylated NAD, 5 x 500 µl |
|
4670-500-10 |
Biotinylated NAD, 10 x 500 µl |
 F
NAD for use with enzymes requiring NAD as a substrate or
cofactor. A number of proteins, including poly (ADP-ribose)
polymerase (PARP), and the SIR2 family of NAD(+)-dependent
histone/protein deacetylases, use NAD as a substrate for their
function. FITC conjugated NAD permits the direct measurement of
PARP and other NAD-dependent enzymes by fluorescence microscopy
or by incorporation of fluorescent-labeled poly (ADP-ribose) or
O-acety-ADP ribose onto histones attached to a 96 well plate.
|
| |
| • |
Activity measurements for
NAD requiring enzymes |
| • |
Assays to identify
inhibitors of NAD-requiring enzymes |
| • |
Cell Permeabilization
reagent, 4674-250-01, required for in-cell assays |
|
| |
| Provided in solution at a
concentration of 250 µm in deionized water. There is a 1:1
stoichiometry for incorporation; one FITC label for each NAD
molecule. |
| |
| 21,000 at 265nm |
| |
95% by HPLC
|
| |
| Store at -80°C in working aliquots
to avoid repeated freeze-thawing. |
Products
|
4673-500-01 |
FITC-NAD, 250 µl |
|
4673-500-05 |
FITC-NAD, 5 x 250 µl |
|
4673-500-10 |
FITC-NAD, 10 x 250 µl |
|
Trevigen now offers two grades of poly (ADP-ribose) polymerase:
a “standard” PARP, and a new high specific activity (HSA)
enzyme. The specific activity serves as a measure of enzyme
purity, with the standard enzyme being purified to approximately
50% purity, and the high specific activity enzyme to greater
than 95%. The standard PARP enzyme is useful as a positive
control for both Western blot analysis of ribosylated proteins
and the PARP Activity Assay (Cat# 4667-50-K). For more detailed
studies or for drug discovery applications, the HSA PARP is
recommended. The catalytic domain of PARP is also available.
|
| |
| One unit is the amount of enzyme
required to incorporate 100 pmol of poly(ADP) from NAD into
acid-insoluble form in 1 minute at 22°C. |
| |
| Purified from E. coli
containing a recombinant plasmid encoding the full-length human
PARP gene. |
| |
| • |
Identification of
inhibitors and activators of PARP activity
|
| • |
Quantitation of DNA damage
|
| • |
Investigation of PARP
inactivation during apoptosis |
|
| |
| Freeze in working aliquots at -80 C
to avoid repeated freeze-thawing. |
Products
|
4667-50-01 |
PARP Enzyme, 100 Units |
|
4667-250-01 |
PARP Enzyme, 500 Units |
|
4668-100-01 |
PARP Enzyme, High Specific Activity, 100 Units |
|
4668-500-01 |
PARP Enzyme, High Specific Activity, 500 Units |
|
4668-2K-01 |
PARP Enzyme, High Specific Activity, 2000 units |
|
4669-100-01 |
Chicken PARP Catalytic Domain, 100µg |
|
* 4669-100-02 |
Human PARP Catalytic Domain, 100µg |
 3-Aminobenzamide |
3-aminobenzamide is the PARP
inhibitor provided with Trevigen's PARP Inhibition Assay Kit
(Cat# 4669-96-K) at 200 mM.
|
|
4-Amino-1,8-naphthalimide |
4-Amino-1,8-naphthalimide is
a potent inhibitor of poly(ADP-ribose) polymer (PARP) and
reduces ischemia-reperfusion injury in the heart and skeletal
muscle. It is 1000-fold more potent that 3-aminobenzamide and
exhibits mixed-type inhibition with respect to the sub-strate,
NAD+, at micromolar concentrations. This inhibitor, which has
been analyzed at a concentration of 20 µM, is provided at a
convenient concentration for subsequent serial dilution and use
with Trevigen's PARP Inhibition Assay Kit (Cat# 4669-96-K).
|
|
6(5H)-Phenanthridinone |
6(5H)-Phenanthridinone
strongly inhibits poly(ADP-ribose) polymerase (PARP) and
displays immunosuppressive activity. It is a mixed-type
inhibitor that acts on both the enzyme and enzyme-NAD+ complex
at site(s) distinct from the NAD+-binding site to attenuate the
fall in NAD and ATP and, consequently, improve cell survival. It
also inhibits concanavalin A-induced lymphocyte proliferation at
micromolar concentrations. This inhibitor, which has been
analyzed in the range of 0.18-0.39 µM, is provided at a
convenient concentration for subsequent serial dilution and use
with Trevigen's PARP Inhibition Assay Kit (Cat# 4669-96-K).
|
|
Benzamide |
| Benzamide is the most potent
poly(ADP-ribose) polymerase (PARP) inhibitor in the family of
benzamides. It acts as a neuroprotectant since it inhibits PARP,
an enzyme activated by nitric oxide. Benzamide is twice as
active than its commonly used counterpart, 3-aminobenzamide in
delaying or suppressing PARP activation. It is able to prevent
nuclear fragmentation and apoptotic-body formation without
affecting DNA fragmentation during apoptosis. This PARP
inhibitor, which has been analyzed in the range of 100 to 500
µM, is provided at a convenient concentration for subsequent
serial dilution and use with Trevigen's PARP Inhibition Assay
Kit (Cat# 4669-96-K). |
Products
|
4667-50-03 |
3-Aminobenzamide, 60 µl |
|
4667-50-09 |
4-Amino-1,8-naphthalimide 100 µl |
|
4667-50-10 |
6(5H)-Phenanthridinone, 100 µl |
|
4667-50-11 |
Benzamide, 100 µl |
|
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