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BIOTAQ DNA polymerase 500U Ref: M95801B
Product Description
BIO TAQ is a high-performance thermostable DNA polymerase, isolated from Thermus
aquaticus YT-1 as described by Kaledin (2). BIOTAQ is a robust enzyme with very
high activity, which produces excellent yields in primer extension and other
molecular biology applications. BIOTAQ is specially purified to remove traces of
DNA that can interfere with some reactions. BIOTAQ is supplied with 10x NH4
-based reaction buffer, (that provides superior results with minimal
background,) and a separate solution of 50mM MgCl2, which allows users to
tailor-make reaction conditions to particular needs. BIOTAQ has outstanding
activity - some users find that as little as 0.1 unit is enough for their
reactions - which makes BIOTAQ one of the best value polymerases on the market.
Features
· Excellent yields · Highly purified · Superb value
Applications
Standard reactions
Unit Definition
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTPs
into acid-insoluble form in 30 minutes at 72şC under the following assay
conditions: 25mM TAPS (tris-[hydroxymethyl]methyl-amino-propanesulphonic acid,
sodium salt), pH 9.3 at 25şC, 50mM KCl, 2mM MgCl2, 1mM b-mercaptoethanol, 200µM
of each dATP, dGTP, dTTP, 100µM dCTP (a mix of unlabelled and g-[32P]-labelled)
and 12.5µg activated salmon sperm DNA in a final volume of 50µl.
Storage Buffer
20mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 2mM DTT, 50% glycerol, 0.1%
Tween-20.
Reaction Buffers
(10x)160mM (NH4)2 SO4 , 670mM Tris-HCl (pH 8.8 at 25şC), 0.1% Tween-20.
Separate Magnisium Solutions
50mM MgCl2 .
Quality Control Tests
Activity, non-specific endonucleases/nickase, exonucleases
Storage Conditions
BIOTAQ will remain stable at -20şC in a constant-temperature freezer
Price: €93.00
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