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H FABP ELISA

 

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Reagent for quantification of heart-type fatty acid-binding protein (H-FABP) in serum and plasma

For research use only

MARKIT-M H-FABP ELISA

 895 Euro

 

 

Heart-type fatty acid-binding protein (H-FABP) is

a soluble cytoplasmic protein with a low molecular weight of 14.9 kDa which is present           abundantly in the myocardium. When the myocardium is injured, H-FABP is easily released into the circulation even if in superacute phase within 3 hrs after the onset of symptoms. Therefore, H-FABP is thought to be an exellent biomarker for the diagnosis of acute myocardial infarction. MARKIT-M H-FABP is a direct sandwich enzyme-linked immunosorbent assay (ELISA) for the quantification of H-FABP in human serum and plasma using two distinct anti-human H-FABP monoclonal antibodies.

 

 

Application

Quantification of H-FABP in human serum and plasma

 

Assay procedure for MARKIT-M H-FABP

 

Contents of MARKIT-M H-FABP

Each kit (96 tests) contains the following reagents.

Standard 0 (lyophilized): 1 vial (for2.0 mL).

Standard 5 (lyophilized): 1 vial (for 0.5 mL) contains 2.5 ng of H-FABP*.

Standard 10 (lyophilized): 1 vial (for 0.5 mL) contains 5 ng of H-FABP*.

Standard 25 (lyophilized): 1 vial (for 0.5 mL) contains 12.5 ng of H-FABP*.

Standard 50 (lyophilized): 1 vial (for 0.5 mL) contains 25 ng of H-FABP*.

Standard 100 (lyophilized): 1 vial (for 0.5 mL) contains 50 ng of H-FABP*.

Standard 250 (lyophilized): 1 vial (for 0.5 mL) contains 125 ng of H-FABP*.

H-FABP antibody-coated wells: 1 plate (96 wells).  Each well contains: anti-human H-FABP monoclonal antibody.

Buffer solution (bottle No. 1): 1 bottle (10 mL).

Wash buffer concentrate (bottle No. 2): 3 bottles (30 mL each).

H-FABP antibody-enzyme conjugate (bottle No. 3): 1 bottle (15 mL), containing HRP-labeled anti-human H-FABP monoclonal antibody.

Substrate tablets: 3.  Each tablet contains o-phenylenediamine dihydrochloride (OPD) (13 mg).

Substrate diluent buffer (bottle No. 4): 3 bottles (15 mL each). Each bottle contains hydrogen peroxide (15μL).

Stop solution (bottle No. 5): 1 bottle (15 mL).

Microplate for dilution: 1 plate (96 wells).

Graph paper: 3 sheets.

*H-FABP: Human H-FABP is quantitatively determined by an immunological method using purified human H-FABP as a standard material.

Performance

1. Reproducibility

When two distinct samples (H-FABP, 50-200 ng/mL) are determined 10 times simultaneously, the coefficient of variation in their absorbances should be less than 10 %.

2. Assay range

H-FABP 1.25 -250 ng/mL

 

Cut-off level of H-FABP

A mass concentration of 6.2 ng of H-FABP per mL of serum or plasma is designated as the cut-off value for the diagnosis of acute myocardial infarction.  For clinical assessment this value shows the highest diagnostic efficacy.  An H-FABP mass concentration of 6.2 ng/mL of serum or plasma or higher is strongly suggestive of cardiac damage.

Storage method and expiry period

Storage: Store in a cool place (2-10oC), protected from light.  Avoid freezing.

Expiry period: 2 years

References

1) Ockner, R.K., et al.: Mol. Cell. Biochem. 98: 3-9, 1990.

2) Kaikaus, R.M., et al.: Experientia 46: 617-630, 1990.

3) Ohkaru, Y., et al.: J. Immunol. Methods 178: 99-111, 1995.

4) Tsuji, R., et al.: Int. J. Cardiol. 41: 209-217, 1993.

5) Sohmiya, K., et al.: J. Mol. Cell. Cardiol. 25: 1413-1426, 1993.

6) Tanaka, T., et al.: Clin. Biochem. 24: 195-201, 1991.

7) Paulussen, R.J.A., et al.: Int. J. Biochem. 22: 393-398, 1990.

8) Adams, J.E. III, et al.: Circulation 88: 750-763, 1993.

9) Pauleo, P.R. and Roberts, R.: Cardiol. Clin. 6: 97-109, 1988.

10) Veerkamp, J.H., et al.: Prog. Lipid Res. 34: 17-52, 1995.

11) Glatz, J.F.C., et al.: Br. Heart J. 71: 135-140, 1994.

12) Wodzig, K.W.H., et al.: Eur. J. Clin. Chem. Clin. Biochem. 35: 191-198, 1997.

 

 

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Last modified: 05/29/09