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Clonagen

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Bioxys

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KITS FOR VETERINARY USE

Hormones

K214C            K207C            K210C            K207H            K210H            K207M            K209




K214С* Canine/Feline free T4 EIA

Thyroid hormones thyroxin (T4) and 3,5,3’-triiodothyronine (T3) exert regulatory influences on growth,
differentiation, cellular metabolism and development of skeletal and organ systems. T4 and T3 in blood
are found both in free and bound form – mostly, they are bound to thyroxin binding globulin (TBG).
Only free forms of T3 and T4 exert hormonal activity also their percentage is very low – 0.3% for T3
and 0.03% for T4.
The concentration of T4 is generally accepted as an index of thyroid function which provide enough
information to differentiate between hyper-, hypo- and euthyroidism.
Elevation of free T4 is found in hyperthyroidism, in the animals with elevated TBG level (pregnancy,
acute or chronic active hepatitis, estrogen-secreting tumors, hereditary elevation of TBG).
Low free T4 is found in hypothyroidism, in the animals with panhypopituitarism, low TBG level
(acromegaly, nephritic syndrome, hypoproteinemia, chronic liver diseases, androgen-secreting tumors,
hereditary reduction).

 

Sample type: serum, plasma                     Incubation: 60’/15’, 370С                       Control sample: 1
Sample volume: 50 μl                               Calibrators: 6 (0-80 pmol/l)                    Shelf life: 12 months
Sensitivity: 1.5 pmol/l

 


K207C* Canine/Feline Progesterone EIA

Progesterone is a gestagen with a MW of 314.5 Dalton. Progesterone is secreted by corpus luteum,
adrenals and testis; it plays a role of a precursor for corticosteroids and androgens. Being an estrogen
antagonist, Progesteron induces characteristic changes in endometrium necessary for implantation of
an impregnated ovum.

 

Sample type: serum, plasma                     Incubation: 120’/15’, RT                         Control sample: 1
Sample volume: 50 μl                               Calibrators: 7 (0-300 nmol/l)                  Shelf life: 12 months
Sensitivity: 0.5 nmol/l

 

K209 Testosterone EIA

Testosterone is a steroid with a MW of 288.4 Dalton. The main sites of
testosterone secretion are Leidig cells in interstitial tissue of testicles in
men. In women testosterone is secreted in the adrenals and is controlled
by luteinizing hormone. Testosterone stimulates development of male genital
organs and formation of secondary sexual features.
In males, Testosterone secretion undergoes circadian rhythms with maximal
concentrations seen in the morning (6 am) and minimal – in the evening (8 pm). In females, Testosterone
secretion is regulated by menstrual cycle with maximal levels found in luteinic phase and during
ovulation.
Leidig cell tumors producing high levels of serum testosterone in young boys lead to development
of “little Hercules” syndrome. Elevated testosterone level in women causes the clinical signs of
masculinization.
In men, decreased Testosterone levels may lead to female habitus or underdevelopment of male
genital organs in boys. To differentiate between primary and secondary hypogonadism, Testostrerone
should be assayed in conjunction with LH and FSH.

 


Sample type: serum, plasma                     Incubation: 120’/15’, 370С             Control sample: 2
Sample volume: 25 μl                               Calibrators: 6 (0-40 nmol/l)           Shelf life: 12 months
Sensitivity: 0.3 nmol/l

 

K210C* Canine/Feline Cortisol EIA

Cortisol is a glucocorticoid with a MW of 362.5 Dalton. Cortisol is the major hormone secreted by adrenals.
In blood, cortisol is found mostly in a bound form, transcortin being the carrier. Cortisol secretion
undergoes circadian rhythms with maximal (up to 700 nmol/l) concentrations seen in the morning
(6-9 am) and minimal (up to 55 nmol/l) – in the midnight.
During pregnancy, Cortisol blood level is continuously increasing by up to 5-fold of initial concentration before
delivery, its circadian rhythm being altered. Cortisol plays an important role in development of alveolar
epithelium and surfactant secretion, this being of major importance for the first inhale of a newborn.
Elevated Cortisol concentrations in blood are found in secreting tumors of adrenals, in virilizing
hyperplasia of adrenals, in ACTH-producing tumors, during surgical stress, in cardiac insufficiency,
diabetes, burns, pains, during pregnancy, etc.
Decreased Cortisol levels are found in hypopituitarism. Decreased Cortisol level during pregnancy may
indicate anencephaly of the fetus.

 

Sample type: serum, plasma                     Incubation: 60’/15’, 370С               Control sample: 1
Sample volume: 10 μl                               Calibrators: 6 (0-2000 nmol/l)        Shelf life: 12 months
Sensitivity: 25 nmol/l
 

 

K207Н* Horse Progesterone EIA

Progesterone is a gestagen with a MW of 314.5 Dalton. Progesterone is secreted by corpus luteum,
adrenals and testis; it plays a role of a precursor for corticosteroids and androgens. Being an estrogen
antagonist, Progesteron induces characteristic changes in endometrium necessary for implantation
of an impregnated ovum.
During pregnancy, Progesterone concentration continuously increases at least by week 10-12, and
it induces proliferation and development of mammary glands and inhibits ovulation.
Determination of Progesterone in horse serum may be used for early pregnancy detection (within
4 weeks of gestation).

 

Sample type: serum, plasma                     Incubation: 120’/15’, 370С             Control sample: 1
Sample volume: 25 μl                               Calibrators: 6 (0-100 nmol/l)          Shelf life: 12 months
Sensitivity: 0.5 nmol/l
 

 

K210H* Horse Cortisol EIA

This kit is designed for analysis of equine Cortisol in horse serum. The kit contains the reagents
sufficient for 96 determinations and allows to analyze 41 unknown samples in 2 parallels.


Sample type: serum, plasma                     Incubation: 120’/15’, 370С             Control sample: 1
Sample volume: 20 μl                               Calibrators: 6 (0-1000 nmol/l)        Shelf life: 12 months
Sensitivity: 5 nmol/l

 


K207M* Progesterone in Cow’s Milk EIA

Progesterone is a gestagen with a MW of 314.5 Dalton. Progesterone is secreted by corpus luteum,
adrenals and testis; it plays a role of a precursor for corticosteroids and androgens. Being an estrogen
antagonist, Progesteron induces characteristic changes in endometrium necessary for implantation
of an impregnated ovum. If cow is fertilized, progesterone serum level undergoes continuous rise
during 10-12 weeks and induce proliferation and development of mammary glands as well as inhibits
ovulation.
Some proportion of progesterone from serum penetrates into milk, thus enabling atraumatic evaluation
of cow’s pregnancy state.

 

Sample type: milk                                   Incubation: 60’/15’, 370С                Normal range (ng/ml): - cow with calf >7
Sample volume: 20 μl                              Calibrators: 6 (0-100 ng/ml)                                      - cow w/o calf <4
Sensitivity: 1.0 ng/ml                              Control: 1                                                               - “grey zone” 4-7
Shelf life: 12 months                                                                                                   
 

 

 

* - the kits will be made to order.


 

 

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Last modified: 05/29/09