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Mouse Anti Flag-tag


Rabbit Anti Flag-tag


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Cat. No. Size Price
A00013-500 ug
500 ug € 260
A00013-1 mg
1 mg € 455
Full Name Mouse Anti Flag-tag (monoclonal)
Abbreviated Name-1 Anti Flag-tag (monoclonal)
Abbreviated Name-2 Anti Flag-tag
Description Anti Flag-tag monoclonal antibody is a purified IgG2b monoclonal antibody isolated from Murine ascites fluid that binds to proteins with a Flag marker at free N-terminus or C-terminus. It is useful for identification of proteins with a Flag marker at free N-terminus or C-terminus by common immunological applications in yeast, animal and E. coli cells. Anti Flag-tag monoclonal antibody is also useful for calcium mediated affinity purification of proteins with a Flag marker at either free N-terminus or C-terminus when bound to a solid support.
Host Species Mouse.
Immunogen A synthetic peptide (DYKDDDDK) coupled to KLH.
Specificity Recognises over-expressed proteins containing Flag epitope tag fused to either amino- or carboxy-terminal of targeted proteins.
Ig Subclass Mouse Monoclonal (IgG2b).
Clone A00013.01
Conjugation Unconjugated.
Concentration Lyophilized powder. Reconstitute it with sterile water to 1 mg/ml.
Storage Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
Applications Western Blot (1:1,000), Immunoprecipitation and Immunostaining (1:200).
Note Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

* For Non-Clinical Research Use Only *

Mouse Anti Flag-tag (monoclonal) Western Blot
MSDS:  20051003104711  (PDF)

  • Schuster M, et al. Protein expression strategies for identification of novel target proteins. J Biomol Screen. Apr 2000; 5(2): 89-97.
  • Nielsen UB, et al. Targeting of bivalent anti-ErbB2 diabody antibody fragments to tumor cells is independent of the intrinsic antibody affinity. Cancer Res. Nov 2000; 60(22): 6434-6440.
  • Kimura M, et al. Formation of a carboxy-terminal domain phosphatase (Fcp1)/TFIIF/RNA polymerase II (pol II) complex in Schizosaccharomyces pombe involves direct interaction between Fcp1 and the Rpb4 subunit of pol II. Mol Cell Biol. Mar 2002;22(5):1577-1588.
Related Products:
  • Precast Gels
    Code Name Size Price  
    M0005 Lysates : Flag lysates 100 ug € 93

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    Last modified: 05/29/09