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Mouse Anti GST-tag

 

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Rabbit Anti GST-tag

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Clonagen

Gentaur

Genprice

Bioxys

Labprice

Cat. No. Size Price
A00014-200 ug
 
200 ug € 182
Full Name Mouse Anti GST-tag (monoclonal)
Abbreviated Name-1 Anti GST-tag
Abbreviated Name-2 Anti GST
Description Schistosoma Japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expresses in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione, and the GST portion of the fusion protein can be separated from the protein of interest by using site specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.
Host Species Mouse.
Immunogen Recombinant GST protein.
Specificity Mouse Anti GST-tag antibody detects over expressed glutathione-transferase (GST) fusion proteins.
Fusion Partner Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells.
Ig Subclass Mouse IgG2b.
Clone A00014.01
Purification Method Protein G affinity column.
Conjugation Unconjugated.
Concentration 1 mg/ml in PBS, pH 7.4 with 0.02% sodium azide. Frozen liquid.
Storage Store at 4°C short term. Aliquot and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Applications Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detectionmethod, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concantration ranges are ecommended starting points for this product:
ELISA: 0.01~0.1 µg/ml,
Western blot: 1.0 µg/ml.
Note Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

 
* For Non-Clinical Research Use Only *


EXAMPLE:
Mouse Anti GST Western Blot
Zoom
MSDS:  20051003104711  (PDF)

 
Reference:
  • Matsuoka D, Tokutomi S. Blue light-regulated molecular switch of Ser/Thr kinase in phototropin. Proc Natl Acad Sci U S A. Sep 2005; 102(37): 13337-13342.
  • Finnen RL, et al. Interactions between papillomavirus L1 and L2 capsid proteins. J Virol. Apr 2003; 77(8): 4818-4826.
  • Kozak NA, et al. Interactions between partner switcher orthologs BtrW and BtrV regulate type III secretion in Bordetella. J Bacteriol. Aug 2005;187(16):5665-5676.
Related Products:
  • Precast Gels
    Code Name Size Price  
    M0001 Lysates : GST lysates 100 ug € 93  
    A00097 antibody : Rabbit Anti GST-tag (polyclonal) 200 ug € 208
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    Last modified: 05/29/09