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0032 (0)16 41 44 07
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| Cat. No. |
Size |
Price |
A00014-200 ug
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200 ug |
€ 182 |
| Full Name |
Mouse Anti GST-tag
(monoclonal) |
| Abbreviated Name-1 |
Anti GST-tag |
| Abbreviated Name-2 |
Anti GST |
| Description |
Schistosoma Japonicum
glutathione S-transferase (GST) is a 26,000 Da enzyme that
conjugates reduced glutathione to hydrophobic electrophiles. GST
is an ideal fusion partner for production of foreign proteins in
commercially available expression systems because it can be
expresses in high levels in E. coli. The fusion proteins
can be purified to homogenity in a single step as the GST
portion of the protein binds tightly to immobilized glutathione.
Elution can be accomplished using free glutathione, and the GST
portion of the fusion protein can be separated from the protein
of interest by using site specific proteases. Antibodies
directed against GST can be helpful in detecting the fusion
protein during purification and to detect cleavage of GST from
the protein of interest. |
| Host Species |
Mouse. |
| Immunogen |
Recombinant GST protein. |
| Specificity |
Mouse Anti GST-tag antibody
detects over expressed glutathione-transferase (GST) fusion
proteins. |
| Fusion Partner |
Spleen cells were fused with
SP2/0-Ag14 mouse myeloma cells. |
| Ig Subclass |
Mouse IgG2b. |
| Clone |
A00014.01 |
| Purification Method |
Protein G affinity column. |
| Conjugation |
Unconjugated. |
| Concentration |
1 mg/ml in PBS, pH 7.4 with 0.02%
sodium azide. Frozen liquid. |
| Storage |
Store at 4°C short term. Aliquot
and store at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
For maximum recovery of product, centrifuge the original vial
after thawing and prior to removing the cap. |
| Applications |
Working concentrations for
specific applications should be determined by the investigator.
Appropriate concentrations will be affected by several factors,
including secondary antibody affinity, antigen concentration,
sensitivity of detectionmethod, temperature, and length of
incubations, etc. The suitability of this antibody for
applications other than those listed below has not been
determined. The following concantration ranges are ecommended
starting points for this product:
ELISA: 0.01~0.1 µg/ml,
Western blot: 1.0 µg/ml. |
| Note |
Briefly centrifuge the vial on a
tabletop centrifuge to dislodge any liquid in the container's
cap because small volumes of antibody will occasionally become
entrapped in the seal of the product vial during shipment and
storage. |
* For Non-Clinical Research Use Only *
EXAMPLE:
Zoom
MSDS: 20051003104711 (PDF)
Reference:
- Matsuoka D, Tokutomi S. Blue light-regulated molecular switch of
Ser/Thr kinase in phototropin. Proc Natl Acad Sci U S A. Sep 2005;
102(37): 13337-13342.
- Finnen RL, et al. Interactions between papillomavirus L1
and L2 capsid proteins. J Virol. Apr 2003; 77(8): 4818-4826.
- Kozak NA, et al. Interactions between partner switcher
orthologs BtrW and BtrV regulate type III secretion in Bordetella. J
Bacteriol. Aug 2005;187(16):5665-5676.
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