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Mycoplasma PCR Detection Kit

 

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e-MycoTM Mycoplasma PCR Detection Kit (ver.2.0, for 20ul

Ordering Information

Protocol
Cat. No Size
25233 96 Tubes
 
 
The e-MycoTM (ver.2.0) product is a set of primers designed to detect the presence of mycoplasma that might contaminate biological materials such as cultured cells. Conventional techniques that are used to detect mycoplasma involve culturing samples on selective media, which needs at least 1 week to obtain results, whereas by performing PCR with this primer set, which is based on conserved 16S rRNA, detection results are obtained in a few hours. Because the presence of contaminant mycoplasma can be easily detected by only verifying the bands of amplified DNA fragments using electrophoresis, there is no need to prepare probes that are labeled with radioisotope, etc. The adopted 8-methoxypsoralen (8-MOP) is used to extinguish the template activity of contaminated DNAs.

An internal control of this product was constructed to identify false negative results in each reaction. The internal control was designed in such a way that the sample primer pair was used to amplify the internal control and target DNA, which were differentiated by size. Each tube of the e-Myco Mycoplasma PCR Detection Kit (ver.2.0) contains all the components for PCR except for template: i-StarTaqTM DNA Polymerase, dNTPs, 10x Buffer, primers, 8-MOP, and internal control for Mycoplasma partial gene amplifications. So, you can just add your templates and perform the PCR reaction.

 
Premix Type 
- This e-Myco Mycoplasma PCR Detection Kit(ver.2.0) contains all the components for the PCR reaction. You just add template DNA or samples.
Broad Species Detection
- You can detect five common cell culture-infecting species of mycoplasma and  also other various species of mycoplasma (See Technical Guide).  
Species Determination
- You can determine the species of mycoplasma by sequencing the amplified  PCR products. 
Internal Control
- Internal control embedded in the product prevents misjudgment that possibly arises from an erroneous PCR test.
Elimination of Carryover Contamination System
-   8-MOP solution prevents carryover contamination by PCR products.

 

 

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Copyright 2002 GENTAUR Molecular Products
Last modified: 05/29/09