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Clonagen

Gentaur

Genprice

Bioxys

Labprice

Product Number
011311H12
Antibody specificity
Anti-mouse Lgi4
Clone Number
KT18
Isotype
Rat IgG1
Antibody form
Intact antibody
Concentration
1 mg/ml in PBS containing 0.1% sodium azide as a preservative
Cross-reactivity
Not tested
Applications
Western blotting, immunofluorescent staining. These are applications that have been tested. It does not mean that the antibody cannot be used for other applications.
Figure 1. Lgi4 is expressed in Purkinje cells of the cerebellum when detected by KT18. Cryo-section was from adult mouse cerellum. Courtesy of Dr. Dies Meijer.
Figure 2. Western blot of whole COS cell extracts using KT18. 1. Cells were transfected with HA-Oct6 as negative control; 2. Cells were transfected with Lgi4-myc expression cassette. Courtesy of Dr. Dies Meijer.
Suggested working concentrations
Western blotting: starting from 1:2000, dilution range 1:500-1:5000; immunofluorescent staining: starting from 1:200, dilution range 1:100-1:1000.
Preparation
Antibody is purified from culture supernatant using a protein G column
Store
4 °C for 12 months from date of despatch, -20 °C in aliquots for 2 years
Safety information
This product contains sodium azide which is hazardous

Figure 1. Lgi4 is expressed in Purkinje cells of the cerebellum when detected by KT18. Cryo-section was from adult mouse cerellum. Courtesy of Dr. Dies Meijer.
Figure 2. Western blot of whole COS cell extracts using KT18. 1. Cells were transfected with HA-Oct6 as negative control; 2. Cells were transfected with Lgi4-myc expression cassette. Courtesy of Dr. Dies Meijer.

 

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Last modified: 05/29/09