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Description : |
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Recombinant Human
Thrombopoietin produced in
E.Coli is a single,
non-glycosylated polypeptide
chain containing 174 amino
acids and having a molecular
mass of 18608 Dalton which
comprises the receptor
binding domain of the
Mpl-ligand protein.
Recombinant Human
Thrombopoietin is purified
by proprietary
chromatographic techniques.
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Physical Appearance:
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Sterile Filtered White
lyophilized (freeze-dried)
powder.
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Formulation:
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Lyophilized from a
concentrated (1mg/ml)
solution in water containing
no additives.
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Solubility:
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It is recommended to
reconstitute the lyophilized
Recombinant TPO in sterile
18MΩ-cm H2O not less than
100µg/ml, which can then be
further diluted to other
aqueous solutions.
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Stability:
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Lyophilized Recombinant TPO
although stable at room
temperature for 3 weeks,
should be stored desiccated
below -18 C. Upon
reconstitution Recombinant
TPO should be stored at 4 C
between 2-7 days and for
future use below -18 C. For
long term storage it is
recommended to add a carrier
protein (0.1% HSA or BSA).
Please avoid freeze-thaw
cycles.
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Purity:
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Greater than 98.0% as
determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and
non-reducing SDS-PAGE Silver
Stained.
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Amino-Acid Sequence :
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The sequence of the first
five N-terminal amino acids
was determined and was found
to be Ser-Pro-Ala-Pr-Pro.
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Dimers and aggregates:
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Less than 1% as determined
by silver-stained SDS-PAGE
gel analysis.
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Biological Activity:
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ProSpec’s Recombinant TPO is
fully biologically active
when compared to standard.
The ED50 as determined by
the dose-dependant
stimulation of MO7e cells is
less then 1 ng/ml,
corresponding to a Specific
Activity of 1 x 10 6
IU/mg.
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Endotoxin:
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Less than 0.1 ng/µg (IEU/µg)
of TPO.
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Protein content:
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Protein quantitation was
carried out by two
independent methods:
1. UV spectroscopy at 280
nm.
2. Analysis by RP-HPLC,
using a calibrated solution
of TPO as a Reference
Standard.
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Usage:
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Prospec's products are
furnished for LABORATORY
RESEARCH USE ONLY. The
product may not be used as
drugs, agricultural or
pesticidal products, food
additives or household
chemicals.
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+32 1658 9045
+32 1650 9045
