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Recombinant Human TPO

rhuTPO

 
1000ug 3900 Euro

 

Recombinant Human Thrombopoietin (TPO)

Certificate of Analysis and Data Sheet
 

 
Source:
E.Coli
Catalog No.
CYT-302

 
Description :
  Recombinant Human Thrombopoietin produced in E.Coli is a single, non-glycosylated polypeptide chain containing 174 amino acids and having a molecular mass of 18608 Dalton which comprises the receptor binding domain of the Mpl-ligand protein.
Recombinant Human Thrombopoietin is purified by proprietary chromatographic techniques.

 
Physical Appearance:
  Sterile Filtered White lyophilized (freeze-dried) powder.

 
Formulation:
  Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Solubility:
  It is recommended to reconstitute the lyophilized Recombinant TPO in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

 
Stability:
 
  Lyophilized Recombinant TPO although stable at room temperature for 3 weeks, should be stored desiccated below -18 C. Upon reconstitution Recombinant TPO should be stored at 4 C between 2-7 days and for future use below -18 C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.

 
Purity:
  Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained.

 
Amino-Acid Sequence :
  The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Pro-Ala-Pr-Pro.

 
Dimers and aggregates:
  Less than 1% as determined by silver-stained SDS-PAGE gel analysis.

 
Biological Activity:
  ProSpec’s Recombinant TPO is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of MO7e cells is less then 1 ng/ml, corresponding to a Specific Activity of 1 x 10 6 IU/mg.

 
Endotoxin:
  Less than 0.1 ng/µg (IEU/µg) of TPO.

 
Protein content:
  Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm.
2. Analysis by RP-HPLC, using a calibrated solution of TPO as a Reference Standard.

 
Usage:
  Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.

 

 
Gene:
  Name:THPO

 
Protein synonyms/aliases:
  Thrombopoietin precursor (Megakaryocyte colony-stimulating factor)
    (Myeloproliferative leukemia virus oncogene ligand)
    (C-mpl ligand)
    (ML)
    (Megakaryocyte growth and development factor)
    (MGDF).

 
Protein Family:
  Belongs to the EPO/TPO family.

 
Protein Domains:
 
Domains:


 
Related proteins:
  Recombinant Human Erythropoietin-alpha
Recombinant Murine Thrombopoietin


 
Protein links:
 
Recombinant Human Thrombopoietin protein domain Recombinant Human Thrombopoietin protein family


 
Precursor- Protein structure and amino acid sequence:
  Recombinant Human Thrombopoietin amino acid sequence

 
Latest Publications:
  1. Thrombopoietin and interleukin-6 levels in Henoch-Schonlein purpura.

J Microbiol Immunol Infect 2006 Dec;39(6):476-82

2.
Thrombopoietin contributes to enhanced platelet activation in patients with unstable angina.

J Am Coll Cardiol 2006 Dec 5;48(11):2195-203

3.
Complex splicing control of the human Thrombopoietin gene by intronic G runs.

Nucleic Acids Res 2007;35(1):132-42

4.
Clinical approaches involving thrombopoietin to shorten the period of thrombocytopenia after high-dose chemotherapy.

Transfus Med Rev 2006 Oct;20(4):283-93

5.
A family with hereditary thrombocythaemia and normal genes for thrombopoietin and c-Mpl.

Br J Haematol 2006 Nov;135(3):348-51

6.
A rationally designed agonist antibody fragment that functionally mimics thrombopoietin.

Proc Natl Acad Sci U S A 2006 Sep 26;103(39):14307-12

 

 

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Last modified: 05/29/09