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One-StepTM Western kit cuts the lengthy Western Blot Analysis down to one hour.
(Cat. No. L00204C10 and L00205C10)
 Easy to perform  No secondary antibody is needed
 High sensitivity  Reproducible results
 Detect any protein  No special labelling is required
Try this breakthrough technology today, and turn waiting into knowing!

 
Technology Description
One-Step Western Blot analysis flow chart One-StepTM Western Kit is one of GenScript newly introduced products based on GenScripts proprietary innovative technologies. Instead of using classical three-step procedure (blocking, primary antibody-binding and secondary antibody-binding) that takes about 4.5 hrs, these kits allow our customers to perform Western blot or Dot blot within an hour. After transferring proteins from a gel to the membrane (or using dot blot membrane), you simply incubate the membrane in Pretreatment Solution for 5 minutes and in WB solution with primary antibody added for 40 min, and wash the membrane three times for 5 min each, then you are ready to develop the blots using either Chemiluminescent substrate or TMB. This kit can be used for Thrombospondin ELISA Research.

 
Key features
  • Easy to perform: simple and rapid procedure to perform Western-Blot or Dot-Blot in an hour.
  • High sensitivity: the sensitivity using these kits is comparable with or better than that obtained from the classical 4.5 hr procedure (depending on the quality and amount of antibodies used).
  • Reproducible results: the kit produces highly reproducible results.
  • Much less optimization is needed compared with the classical method.
  • No secondary antibody is needed.
  • No special labeling is required for primary antibody.

Time analysis on Western Blot Analysis

 
Western Blotting Kit Selection Guide

To achieve the best result, we highly recommend to use the "Complete" Kits which include both membrane and reagents for blot development. The "Complete" Kits eliminate variability in results due to membrane & development reagents.

 

 
Key Products
Cat. No. Name Size Price
L00204 One-StepTM Western Kit (Rabbit) 1 Kit (10 Assays) 280
L00205 One-StepTM Western Kit (Mouse) 1 Kit (10 Assays) 280
L00204C10 One-StepTM Complete Western Kit (Rabbit) 1 Kit (10 Assays) 385
L00204C50 One-StepTM Complete Western Kit (Rabbit) 1 Kit (50 Assays) 1280
L00205C10 One-StepTM Complete Western Kit (Mouse) 1 Kit (10 Assays) 385
L00205C50 One-StepTM Complete Western Kit (Mouse) 1 Kit (50 Assays) 1280
L00226 One-StepTM Complete Western Kit (Chicken) 1 Kit (10 Assays) 385
L00227 One-StepTM Complete Western Kit (Rat) 1 Kit (10 Assays) 385
L00228 One-StepTM Complete Western Kit (Goat or Sheep) 1 Kit (10 Assays) 385
L00204T One-StepTM Western Kit using TMB (Rabbit) 1 Kit (10 Assays) 385
L00205T One-StepTM Western Kit using TMB (Mouse) 1 Kit (10 Assays) 385
L00215 One-StepTM Flag-Tag Western Kit 1 Kit (10 Assays) 505
L00211 One-StepTM GST-Tag Western Kit 1 Kit (10 Assays) 505
L00214 One-StepTM HA-Tag Western Kit 1 Kit (10 Assays) 505
L00212 One-StepTM His-Tag Western Kit 1 Kit (10 Assays) 505
L00213 One-StepTM Trx-Tag Western Kit 1 Kit (10 Assays) 505
L00224A60 WestClear Nitrocellulose Membrane 1 pk (10 sheets of 7.5 x 8 cm) 105
L00221V60 LumiSensorTM Chemiluminescent HRP Substrate Kit 1 Kit (2 X 30 ml) 100
L00222V60 ChromoSensorTM One Solution TMB Substrate 1 pk (60 ml) 65

 

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Last modified: 05/29/09