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Assay Kits
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Oxidative DNA Biomarkers
Catalog: NWK-8OHDG01
NWLSS™ Urinary 8-OHDG ELISA
8-hydroxy-2’-deoxyguanosine (8-OHdG) is formed when DNA is oxidatively modified
by reactive oxygen species (ROS). 8-OHdG is one of the most sensitive biomarkers
for oxidative stress and can be detected in various biological sample types. The
NWLSS™ Urinary 8-OHDG ELISA is intended for quantitative detection of the 8-OHdG
adduct in urine. We recommend product number NWK-8OHDG02 for testing samples
expected to contain lower amounts of 8-OHdG such as plasma, and/or DNA extracts
isolated from cell lysates or tissue homogenates.
Catalog: NWK-8OHDG02
NWLSS™ 8-OHDG ELISA
8-hydroxy-2’-deoxyguanosine (8-OHdG) is formed when DNA is oxidatively modified
by reactive oxygen species (ROS). 8-OHdG is one of the most sensitive biomarkers
for oxidative stress and can be detected in various biological sample types. The
NWLSS™ 8-OHDG ELISA is intended for quantitative detection of the 8-OHdG adduct
in plasma, and/or DNA extracts isolated from cell lysates or tissue homogenates.
. When testing urine samples, same day results are possible when using product
number NWK-8OHDG01. or tissue homogenates.
Oxidative Lipid Biomarkers
Catalog: NWK-HEXL01
NWLSS™ Hexanoyl-Lysine ELISA
Oxidative damage to lipids (lipid peroxidation) has been found to play an
important role in various disease and aging processes. During early stages of
lipid peroxidation, lipid hydroperoxides (LOOH) are formed. These can react
additionally to form later stage end products such as malondialdehyde (MDA) and
hydroxynonenal (HNE). LOOH is sometimes measured to quantify early stage or
acute lipid peroxidation while MDA is commonly measured to quantify late stage
or chronic lipid peroxidation. More recently, it has been reported that
13-hydroperoxyoctadecanoic acid (13-HPODE), a precursor to
13-hydroxyoctadecanoic acid (13-HODE) can react with proteins to form measurable
adducts by covalently binding to specific amino acid residues. The
Hexanoyl-Lysine (HEL) adduct is formed upon oxidative modification of omega-6
fatty acids such as linoleic acid, the predominant polyunsaturated fatty acid
(PUFA) in the human diet, and arachidonic acid. HEL may be another useful
biomarker for detecting and quantifying the earlier stages of lipid
peroxidation.
Catalog: NWK-MDA01
NWLSS™ Malondialdehyde Assay
The NWLSS™ Malondialdehyde assay utilizes an improved Thiobarbituric Acid (TBA)
based technology, still the most widely published method for testing lipid
peroxidation in biological samples. The NWLSS™ NWK-MDA01 assay is designed as a
simple, affordable method for testing lipid peroxidation standardized as
malondialdehyde (MDA). Unlike other TBA based assays, the NWLSS™ assay utilizes
lower heating temperatures, antioxidants to prevent lipid peroxidation artifacts
and an improved data reduction method to reduce non-specific TBARS related
background interference.
Catalog: NWK-ISO01
NWLSS™ 8-Isoprostane ELISA Assay
This product is intended for the quantification of 15-isoprostane F2t (also
known as 8-epi-PGF2a, 8-iso-PGF2a or more commonly 8-Isoprostane) and can be
used with urine, serum or tissue samples following solid phase extraction of the
isoprostane-containing fraction. Instructions are also provided for the
quantification of total 15-isoprostane F2t following hydrolysis of
phospholipids. NOTE: Alternatively, urine samples may be analyzed without solid
phase extraction using the NWLSS™ NWK-ISO2 kit.
Catalog: NWK-ISO02
NWLSS™ Urinary 8-Isoprostane Assay
We recommend this assay as a means of quantifying free 15-isoprostane F2t also
known as 8-isoprostane, the best characterized isoprostane, in urine, without
the need to extract the samples prior to testing. NOTE: Plasma, tissue or cell
lysates may be tested using the NWLSS™ NWK-ISO01 assay for isoprostanes.
Detailed extraction procedures are included with the product.
Catalog: NWK-01143
Oxidized Low Density Lipoprotein ELISA (Ox-LDL-EIA Sandwich Format)
96 well sandwich ELISA for the detection of Oxidized LDL in biological samples.
Optimized for human samples however some crossreactivity in rat and pig species
has been noted.
Catalog: NWK-01158
Oxidized Low Density Lipoprotein ELISA (Ox-LDL-EIA Competitive Format)
96 well Competitive ELISA for the detection of Oxidized LDL in biological
samples. The competive (NWK-01158) assay requires less sample dilution and
yields slightly better recovery values than the sandwich (NWK-01143) ELISA. It
is expected to work better in non-human model systems than the Sandwich
(NWK-01143) ELISA.
Oxidative/Nitrosative Protein Biomarkers
Catalog: NWK-PCK01
Protein Carbonyl ELISA
Protein carbonyls are formed by a variety of oxidative mechanisms and can be
used as an index of oxidative injury. Recommended as a means to detect general
protein oxidation in biological fluids such as plasma, serum, bronchoalveolar
lavage, cerebrospinal fluid, cell extracts and other soluble protein samples.
Catalog: NWK-NTR01
NWLSS™ Nitrotyrosine ELISA
The NWLSS™M is recommended for the detection of nitrosylated proteins in plasma
or serum samples. Excellent tool for peroxynitrate related studies and
recommended as a biomarker of protein related oxidative/nitrosative stress
related augmentation.
Antioxidant Biomarkers
Catalog: NWK-TAC01
NWLSS™ Total Antioxidant Capacity against Peroxyl Radical (TAC-Peroxyl) Assay
The NWLSS™ TAC-Peroxyl Antioxidant Capacity kit is intended for the quantitative
measurement of a sample's antioxidant status against peroxyl radical challenge.
This product represents a more concise tool allowing investigators to assess a
sample's antioxidant capacity against a specific, biologically relevant free
radical species. The peroxyl radical is involved in lipid peroxidation and other
biologically significant pathways. This product is useful for testing many types
of samples including blood plasma or serum, CSF, tissue homogenates, cell
lysates, seminal plasma, wine, fruit juices, brewed teas and other botanical
extracts. Data generated is in Trolox equivalents similar to the popular ORAC
assay.
Catalog: NWK-ARC01
NWLSS™ Antioxidant Reductive Capacity (Antioxidant Status) Assay
The NWLSS™ Antioxidant Capacity kit measuring a sample's ability to reduce Cu++
to Cu+ as a means of assessing antioxidant status. The kit is useful as a simple
method to quantify generic antioxidant capacity in many types of aqueous samples
including serum, plasma, cell culture media and food extracts.
Catalog: NWK-GSH01
NWLSS™ Glutathione Assay
The NWLSS™ Glutathione Assay kit is a kinetic, rate based assay for
quantification of total GSH (reduced and oxidized) in a variety of samples
including blood, plasma, tissues and cultured cells. The high sensitivity method
provides a limit of detection down to 0.25 mM, suitable for measurement of
oxidized GSH (GSSG) after scavenging of reduced GSH.
Catalog: NWK-GPX01
NWLSS™ Glutathione Peroxidase Assay
The NWLSS™ Glutathione Peroxidase Assay kit is a kinetic, rate based assay for
quantification of glutathione peroxidase (GPx) in a variety of samples including
blood products, tissues and cultured cells.
Catalog: NWK-SOD01
NWLSS™ Human Cu/ZnSOD ELISA
The NWLSS™ Cu/ZnSOD ELISA is intended for the quantitative detection of human
Cu/ZnSOD in human plasma, serum cell culture supernatants, erythrocyte (RBC) or
other cell lysates and other biological fluids where SOD may be present.
Catalog: NWK-SOD02
NWLSS™ Cu/Zn Superoxide Dismutase ELISA
Recommended for researchers wishing to assay SOD activity in tissue homogenates,
cell lysates & other biological fluids or extracts where forms of SOD might be
present.
Inflammatory Biomarkers
Catalog: NWK-MPO02
NWLSS™ Human Myeloperoxidase (MPO) ELISA
This kit has been optimized for measurement of MPO in human plasma and tissue
samples. Cross reactivity in rodent model systems has not been tested. MPO has
been implicated as playing a role in the oxidation of low density lipoprotein
(LDL) and the advancement of cardiovascular disease.
Catalog: NWK-HYP01
NWLSS™ Hydrogen Peroxide Assay
This kit is designed to test for hydrogen peroxide content of aqueous biological
samples.
Antibodies
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Oxidative DNA Adduct Biomarkers
Catalog: NWA-8OHdG
Anti-8-Hydroxydeoxyguanosine
Catalog: NWA-5HMdUa & NWA-5HMdUb
Anti-5-Hydroxymethyluridine
Lipid Peroxidation Biomarkers
Catalog: NWA-GMDAa & NWA-GMDAb
Anti-Malondialdehyde
Catalog: NWA-G4HNEa & NWA-G4HNEb
Anti-4-Hydroxynonenal (4-HNE)
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