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Primary cell transfection reagent SAINT-18  
SAINT-18 product description and protocols:

The SAINT-18 delivery system is based on a cationic pyridinium head group, showing excellent bio-compatibility. Molecular structure of SAINT-18 (1-methyl-4-(cis-9-dioleyl)methyl-pyridinium-chlorid):
 

SAINT-18 is dissolved in H2O. Under standard conditions (1 µg DNA complexed with 15 nmol of SAINT-18) a 4 ml package is sufficient to perform transfection experiments with 200 µg DNA.

Stability experiments showed that SAINT-18, stored at 4°-7°C, is stable for at least one year after opening. No loss of function appeared after repeatedly opening of the vials as long as the vials are recapped tightly after use. The antibiotic properties of SAINT-18 ensure sterility for prolonged periods.


SAINT-18 Applications:

A wide variety of mammalian cells can be transfected with SAINT-18, including poor transfectable cell-lines such as primary endothelial cells (HUVEC, HUAEC, H5V). Efficiencies of 15% up to 50% have been achieved with minimal cellular damage.
Other cell types are: HMVEC-dBINeo, HMEC, HCAEC, NHEK, AoSMC, SkMC, PrEC and NHBE. For most optimal transfection results optimization of the standard protocol might be needed.

SAINT-18 is intended for pre-clinical in vivo experiments.
Only SAINT-18GT (Gene Therapy Grade) is suitable for clinical experiments. SAINT-18 is not produced under GMP protocols and is therefore not yet allowed to be used in clinical (human) experiments!

 

ORDERING INFORMATION    
       
SN-1002-01 SAINT-18 1ml 272 €
SN-1002-02 SAINT-18 2ml 380 €
SN-1002-03 SAINT-18 4ml 531 €

 

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Last modified: 05/29/09