SAINT-18 product
description and protocols:
The SAINT-18 delivery system is based on a
cationic pyridinium head group, showing excellent
bio-compatibility. Molecular structure of SAINT-18
(1-methyl-4-(cis-9-dioleyl)methyl-pyridinium-chlorid):
SAINT-18 is dissolved in H2O. Under
standard conditions (1 µg DNA complexed with 15 nmol of
SAINT-18) a 4 ml package is sufficient to perform transfection
experiments with 200 µg DNA.
Stability experiments showed that
SAINT-18, stored at 4°-7°C, is stable for at least one year
after opening. No loss of function appeared after repeatedly
opening of the vials as long as the vials are recapped tightly
after use. The antibiotic properties of SAINT-18 ensure
sterility for prolonged periods.
SAINT-18 Applications:
A wide variety of mammalian cells can be
transfected with SAINT-18, including poor transfectable
cell-lines such as primary endothelial cells (HUVEC, HUAEC,
H5V). Efficiencies of 15% up to 50% have been achieved with
minimal cellular damage.
Other cell types are: HMVEC-dBINeo, HMEC, HCAEC, NHEK, AoSMC,
SkMC, PrEC and NHBE. For most optimal transfection results
optimization of the standard protocol might be needed.
SAINT-18 is intended for pre-clinical in
vivo experiments.
Only SAINT-18GT (Gene Therapy Grade) is suitable for clinical
experiments. SAINT-18 is not produced under GMP protocols and is
therefore not yet allowed to be used in clinical (human)
experiments! |