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Protein Methylation Research

 

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Core Products for Protein Methylation Research

 

ANTI-mRG   -   Methylarginine-Specific Antibody

ANTI-mRG is an IgG-purified rabbit polyclonal antibody that specifically binds methylarginine peptides and proteins from complex biological fluids and cell and tissue extracts

CH3 Catalog # 0800001        Unit Size: 20 g/10 l            224

CH3 Catalog # 0800002        Unit Size: 100 g/50 l          544

 

ANTI-RG   -   Control Antibody Reagent

ANTI-RG is an IgG-purified rabbit polyclonal antibody that specifically recognizes an arginine-rich peptide that is identical to the peptide used to generate ANTI-mRG, except for the replacement of all methylarginine residues with arginine.

CH3 Catalog #0810001         Unit Size: 20 g/10 l            159

CH3 Catalog #0810002         Unit Size: 100 g/50 l          289

 

PRMT1   -   Type I Protein Arginine Methyltransferase

PRMT1 is a recombinant type I protein arginine methyltransferase expressed as a fusion protein in bacteria.  As supplied, the enzyme is active and capable of methylating arginine acceptor sites in peptides and proteins under appropriate incubation conditions.

CH3 Catalog # 0820001        Unit Size: 200 ml    >25 mUnits/min/ml                  1064

 

Arginine-rich Peptide   -   Methylation Substrate

Arginine-Rich Peptide is a synthetic 1.6 kilodalton peptide that can be used to detect arginine methyltransferase activity in cell/tissue extracts and other biological fluids. Detection of the presence of methylarginine can be performed with anti-mRG methylarginine-specific antibody or by film based detection methods that utilize a radioactive methyl donor in the assay reaction.

CH3 Catalog #0830001         Unit Size: 10 g/10 l            159

CH3 Catalog #0830002         Unit Size: 25 g/50 l            289

 

S-Adenosylmethionine  -   pH-stabilized Methyl Donor

Twenty aliquots of 2 mM S-adenosylmethionine (25 ml each) for use in methyltransferase reactions are supplied.  At neutral pH, S-adenosylmethionine is easily oxidized.  This pH-stabilized formulation has been evaluated by CH3 BioSystems to produce methylated product in standard methyltransferase reactions when stored properly for at least one year from the shipping date.

CH3 Catalog # 0890001        Unit Size: 500 l                    159

 

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Last modified: 05/29/09