Several cell lines and
primary cells have been successfully profected with the
SAINT-PhD Reagent,
including CHO-K1, COS-7 , HeLaS3, U373MG, 3T3, HUVEC and Human
Dermal Fibroblasts.
Click below to read more about the great
benefits:
- The unique
advantages of protein delivery with SAINT-PhD.
- Summary of
the key benefits of protein transfection by SAINT-PhD.
- Delivery of
IgG-FITC using SAINT-PhD: comparison of the standard and the
alternative protocol.
- How SAINT-PhD
outperforms the competition.
Recent SAINT-PhD article:
Pajak B, Apoptosis. 2008 Apr;13(4):509-22. Bisindolylmaleimide
IX facilitates extrinsic and
initiates intrinsic apoptosis in TNF-alpha-resistant human colon
adenocarcinoma COLO 205 cells.
(Recombinant biologically functional Bcl-2 protein (R&D Systems
Inc., Minneapolis, MN, USA) was
delivered into COLO 205 cells with SAINT-PhD
[1-methyl-4-(cis-9-dioleyl)methylpyridinium-chloride
(SAINT-18) 1,2-Dioleyl-sn-glycero-3-phosphoethanolamine (DOPE)]
reagent according to the
producer’s instruction (Synvolux Therapeutics, Groningen, The
Netherlands).)
1. Van der Gun BT, J Control Release. 2007
Nov 20;123(3):228-38. Epub 2007 Aug 23, Serum
insensitive, intranuclear protein delivery by the multipurpose
cationic lipid SAINT-2.
(Profection using Saint-2. “SAINT-2:DOPE (SD; 0.75 mM) and
SAINT-2 (S; 0.75 mM) were purchased
from Synvolux Therapeutics Inc.(Groningen, The Netherlands).”)
SAINT-PhD product description and
protocols:
SAINT-PhD consists of a proprietary
cationic pyridinium amphiphile and a helper lipid.
Upon mixture of SAINT-PhD with the protein a particle of
approximately 200nm in diameter is formed. In this particle the
protein is enwrapped by at least one bilayer of lipids.
Furthermore, in the complex formed only non-covalent
interactions are present between SAINT-PhD and the protein. The
cationic amphiphiles on the surface of the particle have high
affinity for the negatively charged cell surface. Upon fusion or
entrapment of the particle the protein is released into the
cytoplasm of the cell. The proteins delivered by SAINT-PhD are
functional and unmodified.
|