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Rabbit Anti GST-tag

 

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Rabbit Anti GST-tag (HRP)

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Clonagen

Gentaur

Genprice

Bioxys

Labprice

Cat. No. Size Price
A00097-200 ug
 
200 ug € 208
Full Name Rabbit Anti GST-tag (polyclonal)
Abbreviated Name-1 Rabbit Anti GST
Abbreviated Name-2 Anti GST
Description Polyclonal Rabbit anti Glutathione-S-Transferase (GST) is highly purified from rabbit antiserum by immunoaffinity chromatography. The immunogen used is a recombinant Glutathione-S-Transferase from Schistosoma japonicum. Glutathione-S-Transferase is a commonly used epitope tag in generating fusion proteins. Antibodies specific for fusion proteins are useful in monitoring protein expression in Western Blots as well as immunoaffinity purification of proteins expressed in E. coli. The Glutathione S-Transferases are a family of enzymes that catalyze the addition of the tripeptide glutathione to endogenous and xenobiotic substrates which have electrophilic functional groups. They play an important role in the detoxication and metabolism of many xenobiotic and endobiotic compounds. The glutathione adducts produced have increased solubility in water and are subsequently enzymatically degraded to mercapturates and excreted.
Host Species Rabbit.
Immunogen Recombinant Glutathione-S-Transferase from Schistosoma japonicum.
Antigen Synonyms Glutathione-S-Transferase Tag.
Antigen Species Schistosoma japonicum.
Specificity This antibody is specific for the GST protein from Schistosoma japonicum and is useful for detecting GST and GST-fusion proteins. Cross-reactivity with cell lysate of E. coli bacteria, sf21 cell and yeast have not been observed.
Ig Subclass Rabbit IgG.
Purification Method Immunoaffinity chromatography.
Conjugation Unconjugated.
Concentration 0.5 mg/ml in PBS, pH 7.4 with 0.02% sodium azide.
Storage Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4°C for 3 months.
Applications Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including secondary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubation, etc. The suitability of this antibody for applications other than those listed here has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05~0.5 µg/ml.
Western blot: 0.1~1 µg/ml.
Other Applications: user optimized.
Note Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

 
* For Non-Clinical Research Use Only *



 
Reference:
  • Kato T, et al. Frequency and significance of anti-glutathione S-transferase autoantibody (anti-GST A1-1) in autoimmune hepatitis. J. Autoimmun. May 2004; 22(3): 211-216.
  • Morari EC, et al. Role of glutathione-S-transferase and codon 72 of P53 genotypes in epithelial ovarian cancer patients. J. Cancer Res. Clin. Oncol. Aug 2006; 132(8): 521-528.
Related Products:
  • Precast Gels
    Code Name Size Price  
    A00130 antibody : Rabbit Anti GST-tag [HRP] (polyclonal) 100 ug € 202  
    L00206 GST Resin 1 kit € 128  
    M0001 Lysates : GST lysates 100 ug € 93  
    L00211 One-StepTM GST Western Detection Kit 1 Kit (10 Assays) € 455
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    Last modified: 05/29/09