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Rickettsia IFA


Rickettsia are intracellular Proteobacteria associated with arthropods, including fleas, body lice, mites and ticks. The antibody response to infections by these agents is primarily directed against a lipopolysaccharide (rLPS) and 1-2 outer-membrane protein (rOmpA and/or rOmpB) antigens. The standard IFA and MIF methods detect antibody against the whole organism and remain the most commonly used of the recommended methods. Due to the range of antigens found on the whole organism, however, this broad reactivity must be compensated for by increasing the cutoff titer to 1:64-1:128. Generally, these methods discriminate between the typhus group and spotted fever group, although titers often differ between the specific infecting species and related species by 2-4-fold. Our MIF slides now offer reference laboratories the ability to make these discriminations at the species level.

SUBSTRATE SLIDES AND KITS:
Rickettsia IFA:
Kit format includes 10 x 12-well Substrate Slides, IFA Conjugate, Controls for each antigen, Mounting Medium, Wash Buffer and Instructions for use.
Individual IFA Slides (propagated in cell culture, as in picture above):
Rickettsia rickettsii (Bitterroot strain)                       
Rickettsia prowazekii (Madrid 22E strain)               
Rickettsia conorii (Moroccan strain)                        
Rickettsia typhi (Wilmington strain)                         
Rickettsia felis
(others available, see list below)                         
Rickettsia IFA Control Sets
Controls are in dropper bottles containing 0.5 mL each. The Positive Control gives strong fluorescence as bottled and should titer 8-fold further. Negative Control is considered non-reactive as bottled.
 
Strains available for IFA Slides
  Rickettsia rickettsii Rickettsia canadensis
  Rickettsia parkeri Rickettsia japonica (regional)
  Rickettsia montanensis Rickettsia honei (Australia, India , N. America)
  Rickettsia conorii (4 subspecies available) Rickettsia slovaca
  Rickettsia akari Rickettsia siberica (Europe, Asia)
  Rickettsia helvetica (North / Central Europe) Rickettsia massiliae (Europe + U.S.)
  Rickettsia africae Rickettsia australis (Australia)
  Rickettsia typhi (endemic typhus) Rickettsia felis (worldwide)
  Rickettsia prowazekii (epidemic typhus)  


 

Product Units Description
IFA Kits
RRG-120 120-test Rickettsia rickettsii IgG Kit                            
RCG-120 120-test Rickettsia conorii IgG Kit                               
RTG-120 120-test Rickettsia typhi IgG Kit                                  
RPG-120 120-test Rickettsia prowazekii IgG Kit                        
IFA Component Products
RC-12 each Rickettsia conorii IFA substrate slides             
RP-12 each Rickettsia prowazekii IFA substrate slides       
RR-12 each Rickettsia rickettsii IFA substrate slides          
RT-12 each Rickettsia typhi IFA substrate slides                
RF-12 each Rickettsia felis IFA substrate slides
TF-GC 1 ea Typhus Group IFA IgG Control Set                   
TF-MC 1 ea Typhus Group IFA IgM Control Set                   
SF-GC 1 ea Spotted Fever Group IFA IgG Control Set        
SF-MC 1 ea Spotted Fever Group  IFA IgM Control Set       

 

Rickettsia MIF


SUBSTRATE SLIDES AND KITS:
Rickettsia MIF:
2-antigen MIF with Rickettsia rickettsii (or Rickettsia conorii) and Rickettsia typhi in each well, for simultaneous detection of spotted fever and typhus fever antibody. Antibody detection by IFA (or MIF) is considered group-specific, predominantly due to the presence of lipopolysaccharide (rLPS) antigens. Thus, in the 2-antigen format, both spotted fever and typhus groups are screened individually and simultaneously in each well.

Between 2- and 6-antigen MIF slides can be produced to your specifications. See list of strains available (below).

Kit format includes 10 x 12-well Substrate Slides, IFA Conjugate, Controls for each antigen, Mounting Medium, Wash Buffer and Instructions for use.

Rickettsia IFA Control Sets
Controls are in dropper bottles containing 0.5 mL each. The Positive Control gives strong fluorescence as bottled and should titer 8-fold further. Negative Control is considered non-reactive as bottled.
 
MIF SUBSTRATE SLIDES (MICRO-IMMUNOFLUORESCENCE) 12-WELLS:
2-6-antigen MIF slides can be produced to your specifications. See list of strains available (below). Other antigens may be used in combination, including Coxiella burnetii, Anaplasma phagocytophilum and Francisella tularensis.
 
Rickettsia strains available for MIF:
Micro-dot arrays within each slide well include up to 6 of the following species:
  Rickettsia rickettsii Rickettsia canadensis
  Rickettsia parkeri Rickettsia akari
  Rickettsia montanensis Rickettsia felis (worldwide)
  Rickettsia conorii (4 subspecies available) Rickettsia slovaca
  Rickettsia akari Rickettsia siberica (Europe, Asia)
  Rickettsia helvetica Rickettsia massiliae (Europe + U.S.)
  Rickettsia africae Rickettsia australis (Australia)
  Rickettsia typhi (endemic typhus) Rickettsia honei (Australia, India , N. America)
  Rickettsia prowazekii (epidemic typhus) Rickettsia japonica (regional)


Catalog Information

Product Units Description
MIF Kits
R20G-120 120-test Rickettsia rickettsii / typhi IgG Kit                
R20M-120 120-test Rickettsia rickettsii / typhi IgM Kit                
R21G-120 120-test Rickettsia conorii / typhi IgG Kit                
R21M-120 120-test Rickettsia conorii / typhi IgM Kit                
IFA Component Products
R20-12 each Rickettsia 2-antigen MIF (12-well)-see info above      
R30-12 each Rickettsia 3-antigen MIF (12-well)-see info above
R21-12 each Rickettsia 2-antigen MIF (12-well)-see info above      
R31-12 each Rickettsia 3-antigen MIF (12-well)-see info above
TF-GC 1 ea Typhus Group IFA IgG Control Set                   
TF-MC 1 ea Typhus Group IFA IgM Control Set                   
SF-GC 1 ea Spotted Fever Group IFA IgG Control Set        
SF-MC 1 ea Spotted Fever Group  IFA IgM Control Set       

 

Rickettsia ELISA


 

ELISA techniques have ranged from whole cell lysates to individual purified antigens, with a range of effects. Our lysate-type antigens have been replaced by two current formats. The rLPS-coated plates often show the earliest antibody response and they also demonstrate the broadest reactivity within the typhus or spotted fever groups. IgM cross-reactivity to other bacteria limits this assay to IgG antibody testing (screening).

The best ELISA data is now seen with the use of the immunodominant outer membrane protein (rOmpB). These proteins are affinity-purified and show excellent group sensitivity, with added sensitivity for the particular species the antigens were isolated from.

Rickettsia species available for ELISA:
  Rickettsia rickettsii Rickettsia canadensis
  Rickettsia parkeri Rickettsia honei (Australia, India , N. America)
  Rickettsia montanensis Rickettsia massiliae (Europe + U.S.)
  Rickettsia conorii (4 subspecies available) Rickettsia slovaca
  Rickettsia akari Rickettsia siberica (Europe, Asia)
  Rickettsia helvetica Rickettsia rhipacephali
  Rickettsia africae Rickettsia amblyommi
  Rickettsia typhi (endemic typhus) Rickettsia felis (worldwide)
  Rickettsia prowazekii (epidemic typhus) Rickettsia australis (Australia)
  Rickettsia japonica (regional)  
     
     
ELISA TEST KITS:
Spotted Fever Rickettsia IgG EIA Assay Kit     
96-test kits (12 x 8-well strip modules) utilize purified rLPS extracted from Rickettsia rickettsii. This kit is recommended for screening, due to its broad sensitivity in detecting Spotted Fever Group IgG-class antibody.
Typhus Fever Rickettsia IgG EIA Assay Kit      
96-test kits (12 x 8-well strip modules) utilize purified rLPS extracted from Rickettsia typhi. This kit is recommended for screening, due to its broad sensitivity in detecting Typhus Group (R. typhi and R. prowazekii) IgG-class antibody.
Rickettsia rickettsii IgG EIA Assay Kit
96-well plates (12 x 8-well strip modules) utilize purified outer membrane antigens (SPA) of Rickettsia rickettsii and are recommended for both IgG and IgM testing,
Rickettsia conorii IgG EIA Assay Kit    
96-well plates (12 x 8-well strip modules) utilize purified outer membrane antigens (SPA) of Rickettsia conorii and are recommended for both igG and IgM testing,
Rickettsia typhi IgG EIA Assay Kit       
96-well plates (12 x 8-well strip modules) utilize purified outer membrane antigen (rOmp B) of Rickettsia typhi and are recommended for both igG and IgM testing,
Rickettsia prowazekii IgG EIA Assay Kit
96-well plates (12 x 8-well strip modules) utilize purified outer membrane antigen (rOmp B) of Rickettsia prowazekii and are recommended for both igG and IgM testing,

 

Product Units Description
EIA Products
SFG-96K 96-tests Spotted Fever Rickettsia EIA IgG Test Kit        
TFG-96K 96-tests Typhus Group Rickettsia EIA IgG Test Kit       
RCM-96K 96-tests Spotted Fever Rickettsia EIA IgM Test Kit       
RRM-96K 96-tests Spotted Fever Rickettsia EIA IgM Test Kit       
RTM-96K 96-tests Typhus Group Rickettsia EIA IgM Test Kit       
RPM-96K 96-tests Typhus Group Rickettsia EIA IgM Test Kit       
RCG-96K each Rickettsia conorii EIA IgG Test Kit
RRG-96K each Rickettsia rickettsii EIA IgG test Kit
RTG-96K each Rickettsia typhi EIA IgG Test Kit
RPG-96K each Rickettsia prowazekii EIA IgG Test Kit
EIA Components
RCS-96 each Rickettsia conorii EIA plate (96-wells)
RRS-96 each Rickettsia rickettsii EIA plate (96-wells)
RPS-96 each Rickettsia prowazekii EIA plate (96-wells)
RTS-96 each Rickettsia typhi EIA plate (96-wells)

 

Rickettsia Monoclonal Antibodies


Mouse monoclonal antibodies are intended for use in immunohistochemistry protocols. Please click on the clone designation for further details.

Clone Immunogen Isotype
RT-61 R. typhi (Wilmington) IgG1
RC-240 R. conorii conorii (Moroccan) IgG1

CA-2A4

R. canadensis (McKeil)

IgG2b

CA-2H10

R. canadensis (McKeil)

IgG2b

CA-3A2

R. canadensis (McKeil)

IgG1

 

 

 

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Last modified: 05/29/09