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SERA

 

Fetal Bovine Serum (FBS)

BIOCHROM AG, Germany offers FBS, originated from four different sources, Australia , USA , EU- and USDA – approved countries. FBS offered by BIOCHROM AG are tested for Endotoxin, Mycoplasma, Bacterial L-forms, Viral contamination, Viral antibodies against BVD-MD, BHV-1, PI-3.

BIOCHROM AG also offers pre-tested FBS lots suitable for Amniotic Fluid cells, Hybridoma cells and Embryonic Stem cells.

Ordering Information
Cat.No Product Units
S 0113 Fetal Bovine Serum (FBS) (origin: EU-approved countries) 100ml
S 0115 Fetal Bovine Serum (FBS) (origin: EU-approved countries) 500ml
S 0213 Fetal Bovine Serum (FBS) (origin: USDA-countries) 100ml
S 0215 Fetal Bovine Serum (FBS) (origin: USDA-countries) 500ml
S 0313 Fetal Bovine Serum (FBS) (origin: USA) 100ml
S 0315 Fetal Bovine Serum (FBS)(origin: USA) 500ml
S 0413 Fetal Bovine Serum (FBS) (origin: Australia) 100ml
S 0415 Fetal Bovine Serum (FBS) (origin: Australia) 500ml

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Cell Culture Media

 

BIOCHROM liquid and powdered cell culture media are standardized according to the original formulation as recommended by the 'Tissue CultureAssociation'.Any modifications from the original formulation are indicated on the label.

Defined cell culture media generally consist of four basic chemical groups: amino acids, carbohydrates, anorganic salts and vitamins. The following is the list of Cell culture media offered by BIOCHROM.

Ordering Information
Cat.No Product Units
F 0915 Alpha Medium with 2.0g/l NaHCO3 with nucleosides 500ml
F 0925 Alpha Medium with 2.0g/l NaHCO3 without nucleosides 500ml
F 0225 BME with Earle's Salts with 2.2g/l NaHCO3 500ml
F 0235 BME with Earle's Salts (10X) without NaHCO3 500ml
F 0245 BME with Hanks' Salts with 0.35g/l NaHCO3 500ml
F 0565 CMRL 1066 with 2.2g/l NaHCO3 500ml
F 0855 Coon's F-12 mod. with 2.5g/l NaHCO3, without L-Glutamine 500ml
F 0405 DMEM with 3.7g/l NaHCO3, without Glucose 500ml
F 0413 DMEM with 3.7g/l NaHCO3, with 1.0g/l Glucose 100ml
F 0415 DMEM with 3.7g/l NaHCO3, with 1.0g/l Glucose 500ml
FG 0415 DMEM with 3.7 g/l NaHCO3, with 1.0g/l Glucose, with stable glutamine 500ml
F 0425 DMEM B723w.3.7g/l NaHCO3, w.4.5g/l Gluc, w/oL-Glut.w/oNa-Pyr.w.20mM HEPES 3 500ml
F 0435` DMEM with 3.7g/l NaHCO3,
with 4.5g/l Glucose w/oNa-Pyruvate.
500ml
FG 0435 DMEM with 3.7g/l NaHCO3, with 4.5g/l Glucose, w.stable glutamine w/o Na-Pyrv 500ml
F 0445 DMEM with 3.7 g/l NaHCO3,
with 4.5 g/l Glucose with Na-Pyruvate.
500ml
FG 0445 DMEM with 3.7g/l NaHCO3, with 4.5g/l Glucose w.stable glutamine w.Na-Pyrv 500ml
F 0455 DMEM (10X) with 4.5g/l Glucose, without NaHCO3, w/oL-Glut., w/o Na-Pyrv 500ml
F 1445 VLE-DMEM (low endotoxn) w 4.5g/l gluc,w
3.7g/l NaHCO3, wNa-Pyr
500ml
FG 1445 VLE-DMEM w 4.5g/l gluc, w 3.7g/l NaHCO3, w Na-Pyr,w stab glut 500ml
F 0475 DMEM with 4.5g/l Glucose, with Na-Pyruvate, w/o Phenol red 500ml
T 041-01 DMEM Powder Medium with 1.0g/l Glucose 1litre
T 041-05 DMEM Powder Medium with 1.0g/l Glucose 5litres
T 041-10 DMEM Powder Medium with 1.0g/l Glucose 10litres
T 041-50 DMEM Powder Medium with 1.0g/l Glucose 50litres
T 043-01 DMEM Powder Medium with 4.5g/l Glucose 1litre
T 043-05 DMEM Powder Medium with 4.5g/l Glucose 5litres
T 043-10 DMEM Powder Medium with 4.5g/l Glucose 10litres
T 043-50 DMEM Powder Medium with 4.5g/l Glucose 50litres
F 4815 DMEM/Ham's F-12 (1:1) with 4.5g/l Glucose 500ml
FG 4815 DMEM/Ham's F-12 (1:1) with 4.5g/l Glucose with stable glutamine 500ml
T 481-01 DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose 1litre
T 481-05 DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose 5litres
T 481-10 DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose 10litres
T 481-50 DMEM/Ham's F-12 (1:1) Powder Medium with 4.5g/l Glucose 50litres
F 0525 Glasgow-MEM with 2.A15675 g/l NaHCO3 500ml
F 0555 Grace's Insect cell medium 500ml
F 0713 Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO3 100ml
F 0715 Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO3 500ml
FG 0713 Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO3, with stable glutamine 100ml
FG 0715 Ham's F-10 with 10mg/l phenol red, with 1.2g/l NaHCO3, with stable glutamine 500ml
F 0723 Ham's F-10 without phenol red, with 1.2g/l NaHCO3, with 10mM HEPES 100ml
T 071-01 Ham's F-10 Powder Medium 1litre
T 071-05 Ham's F-10 Powder Medium 5litres
T 071-10 Ham's F-10 Powder Medium 10litres
T 071-50 Ham's F-10 Powder Medium 50litres
F 0815 Ham's F-12 with 10 mg/l phenol red, with 1.176g/l NaHCO3 500ml
FG 0815 Ham's F-12 with 10 mg/l phenol red, w. 1.176g/l NaHCO3, with stable glutamine 500ml
F 0465 Iscove's Medium, not compl., w.15mg/l phenol red, w. 3.024g/l NaHCO3 500ml
FG 0465 Iscove's Medium, not compl.,w.15mg/l phenol red, w.3.024g/l NaHCO3, w.Glut.B827 500ml
F 1315 L-15 Leibovitz Medium 500ml
F 1013 Mc-COY's 5 A mod. Medium with 2.2 g/l NaHCO3 100ml
F 1015 Mc-COY's 5 A mod. Medium with 2.2 g/l NaHCO3 500ml
F 0613 Medium 199 with Earle's Salts, with 2.2 g/l NaHCO3 100ml
F 0615 Medium 199 with Earle's Salts, with 2.2 g/l NaHCO3 500ml
FG 0615 Medium 199 with Earle's Salts, with 2.2 g/l NaHCO3, with stable glutamine 500ml
F 0633 Medium 199 with Hanks' Salts, with 0.35 g/l NaHCO3 100ml
F 0635 Medium 199 with Hanks' Salts, with 0.35 g/l NaHCO3 500ml
F 0663 Medium 199 with Earle's Salts, with 20mM HEPES without NaHCO3 100ml
F 0665 Medium 199 with Earle's Salts, with 20mM HEPES without NaHCO3 500ml
T 061-01 Medium 199 - Powder Medium with Earle's Salts 1litre
T 061-05 Medium 199 - Powder Medium with Earle's Salts 5litres
T 061-10 Medium 199 - Powder Medium with Earle's Salts 10litres
T 061-50 Medium 199 - Powder Medium with Earle's Salts 50litres
F 0315 MEM with Earle's Salts with 0.85 g/l NaHCO3 500ml
F 0325 MEM with Earle's Salts with 2.2 g/l NaHCO3 500ml
FG 0325 MEM with Earle's Salts with 2.2 g/l NaHCO3 with stable glutamine 500ml
F 0333 MEM with Earle's Salts (10X) without NaHCO3 100ml
F 0335 MEM with Earle's Salts (10X) without NaHCO3 500ml
F 0345 MEM with Hanks' Salts with 0.35 g/l NaHCO3 500ml
F 0383 MEM with Earle's Salts w/o NaHCO3, w/o phenol red 100ml
F 0385 MEM with Earle's Salts w/o NaHCO3, w/o phenol red 500ml
F 4315 MEM with Earle's Salts with 20mM HEPES without NaHCO3 500ml
T 437-01
 
MEM-Powder Medium with Earle's Salts
with non-essential amino acids
1litre
T 437-05
 
MEM-Powder Medium with Earle's Salts
with non-essential amino acids
5litres
T 437-10
 
MEM-Powder Medium with Earle's Salts
with non-essential amino acids
10litres
T 437-50 MEM-Powder Medium with Earle's Salts with non-essential amino acids 50litres
F 1213 RPMI 1640 with 2.0g/l NaHCO3, without L-Glutamine 100ml
F 1215 RPMI 1640 w. 2.0g/l NaHCO3, w/o L-Glutamine 500ml
FG 1213 RPMI 1640 with 2.0g/l NaHCO3, with stable glutamine 100ml
FG 1215 RPMI 1640 with 2.0g/l NaHCO3, w stable glut. (also available as “low endotoxin”) 500ml
F 1223 RPMI 1640 (10x) without NaHCO3 100ml
F 1225 RPMI 1640 (10x) without NaHCO3 500ml
F 1233 RPMI 1640 with 20mM HEPES, without NaHCO3 100ml
F 1235 RPMI 1640 with 20mM HEPES, without NaHCO3 500ml
FG 1233 RPMI 1640 without NaHCO3, with 20mM HEPES, with stable glutamine 100ml
FG 1235 RPMI 1640 without NaHCO3, with 20mM HEPES, with stable glutamine 500ml
F 1245 RPMI 1640 with 2.0g/l NaHCO3, without Methionine 500ml
F 1255 RPMI 1640 with 2.0g/l NaHCO3, with 40mg/l folic acid 500ml
FG 1255 RPMI 1640 with 2.0g/l NaHCO3, with 40mg/l folic acid, with stable glutamine 500ml
F 1275 RPMI 1640 with 2.0g/l NaHCO3 without phenol red 500ml
FG 1383 RPMI 1640 w 2.0g/l NaHCO3, 25mM HEPES, 5.5g/l NaCl, 5mg/l phenol red, stable glut. 100ml
FG 1385 RPMI 1640 w 2.0g/l NaHCO3, 25mM HEPES, 5.5g/l NaCl, 5mg/l phenol red, stable glut. 500ml
F 1295 RPMI 1640 with 2.0g/l NaHCO3, with 5mg/l phenol red 500ml
F 1415 VLE-RPMI 1640 (very low endotoxin), Endotoxin<0,01 IU/ml 500ml
FG 1415 VLE-RPMI 1640 (very low endotoxin) Endotoxin<0,01 IU/ml with stable glut. 500ml
T 121-01 RPMI 1640 - Powder Medium 1litre
T 121-05 RPMI 1640 - Powder Medium 5litres
T 121-10 RPMI 1640 - Powder Medium 10litres
T 121-50 RPMI 1640 - Powder Medium 50litres
F 0545 Insect cell medium TC 100 500ml
F 1115 Williams' Medium E with 2.2g/l NaHCO3, without L-Glutamine 500ml
F 1125 Williams' Medium E with 2.2g/l NaHCO3, without L-Glutamine, without phenol red 500ml

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Complete Culture Media

 

The Complete cell culture media are serum and protein free cell culture media which support the growth of different cells / cell lines. There are specific complete culture media are available to support the growth of cells / cell lines like, human amniotic fluid cells, FRTL 5, human and porcine hepatocytes, hybridomas, lymphocytes, human keratinocytes, CHO cells, VERO cells, sebocytes, neuronal cells, neuroblastoma x glioma hybrid cells, cytotoxicT-cells, chondrocytes, human endothelial cells etc.
Ordering Information
Cat.No Product Units
F 5043 Amniomed 100ml
F 5143 Amniomed with 25 mM HEPES 100ml
F 5013 Chromosome medium A without Phytohemagglutinin (PHA) L 100ml
F 5023 Chromosome A110medium B with Phytohemagglutinin (PHA) E 100ml
F 2235 Chondromed complete 500ml
F 2245 endomed complete 500ml
L 6211 Colchicin, 10 ug/ml in PBS, without Ca, Mg 25ml
L 6221 Colcemide, 10 ug/ml in PBS without Ca, Mg, in plastic bottles 25ml
L 6231 Colcemide, 10 µg/ml in HBSS 25ml.
F 8085
 
octomed serum free CHO-Medium with 2.2 g/l NaHCO3, without L-Glutamine 500ml
F 2225 fibromed complete 500ml
F 2255 heparmed Liver cell Medium 500ml
F 5515 Hybridoma Medium without HAT, with L-Glutamine, with 10% FBS 500ml
F 8035 HybridoMed DIF1000 serum free medium, for 10%CO2 conc., in plastic bottles 500ml
F 8055 HybridoMed DIF1000 serum free+A201medium, for 5%CO2 conc.,in plastic bottl. 500ml
F 8105 MCDB 153 Basal Medium without L-Glutamine 500ml
F 8115 MCDB 153 Basal Medium with L-Glutamine,
with 1.176 g/l NaHCO3
500ml
F 2265 Osteomed complete 500ml
F 8045 PFEK-1, protein free Medium for VERO-cells 500ml
F 8205 sebomed Basal Medium 500ml
F 8215 sebomed Complete Medium 500ml
F 8075 Start V Medium 500ml
F 8023 TNB 100 Medium in plastic bottles 100ml
F 8820 TNB 100, Lipid-Protein-Complex (necessary for 100 ml TNB 100) 2.5ml
F 0483 HAT-Supplement 100ml

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Cell Attachment Factors

 

Growth and differentiation of anchorage dependent cells are strongly influenced by the glass or plastic culture flasks offered as substrate. Cell growth rates can often be improved by surface coating with attachment factors such as fibronectin, collagen, gelatine or polylysine. With a collagen coating, survival time of hepatocytes cab be extended from normally one week to four weeks.

.

Ordering Information
Cat.No Product Units
L 7117 Fibronectin from human plasma 1mg
L 7127 Fibronectin from bovine plasma 1mg
L 7220 Collagen A, 1mg/ml 1 x (6 x 5ml)
L 7213 Collagen G 100ml
L 7230 Gelatine 10mg/ml 1 x (6 x 5ml)
L 7240 Polylysin MG>30.000; 0.1mg/ml 1 x (6 x 5ml)

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Tissue Disintegration Enzymes

 

Cells of animal tissue are attached to each other by a complex matrix of proteins, lipids, glycolipids and muco-polysaccharides. To isolate individual cells or to stat primary cultures, the delicate matrix must be partially broken down without destroying the cell or its surface.

Collagenase are the enzymes of choice for this application, if dissolved in balanced salts.

Ordering Information
Cat.No Product Units
C 1-28 Collagenase type I, CLS 100mg
C 1-22 Collagenase type I, CLS 1g
C 2-28 Collagenase type II, CLS II 100mg
C 2-22 Collagenase type II, CLS II 1g
C 3-28 Collagenase type III, CLS III 100mg
C 3-22 Collagenase type III, CLS III 1g
C 4-28 Collagenase type IV, CLS IV 100mg
C 4-22 Collagenase type IV, CLS IV 1g
L 2113 EDTA (Versen) 1% in PBS without Ca2+, Mg2+ 100ml  
L 2123 Trypsin (1:250) 0.25% in PBS without Ca2+, Mg2+ 100ml
L 2133 Trypsin (1:250) 2.5% in PBS without Ca2+, Mg2+ 100ml
L 2153 Trypsin/EDTA Solution(10X)0.5%/0.2% in (10x) PBS,w/o Ca2+, Mg2+, in pl.B911 bott. 100ml
L 2163 Trypsin/EDTA solution 0.25%/0.02% in PBS without Ca2+, Mg2+ 100ml
L 2103 Trypsin powder substance 100g
L 2223 Papain Solution (6.25mg/l) 100ml

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Seperating Solutions & Lectins

 

Cells of animal tissue are attached to each other by a complex matrix of proteins, lipids, glycolipids and muco-polysaccharides. To isolate individual cells or to stat primary cultures, the delicate matrix must be partially broken down without destroying the cell
or its surface.

Collagenase are the enzymes of choice for this application, if dissolved in balanced salts.

Ordering Information
Cat.No Product Units
L 6113 Biocoll-Separating Solution, density 1.077g/ml isoton 100ml
L 6115 Biocoll-Separating Solution, density 1.077g/ml isoton 500ml
L 6125 Biocoll-Separating Solution, density 1.09g/ml isoton 500ml
L 6155 Biocoll-Separating Solution, density 1.10g/ml isoton 500ml
L 6133 Percoll Separating Solution, density 1.077g/ml isoton 100ml
L 6135 Percoll Separating Solution, density 1.077g/ml isoton 500ml
L 6143 Percoll Separating Solution, density 1.124g/ml isoton 100ml
L 6145 Percoll Separating Solution, density 1.124g/ml isoton 500ml
L 6163 Percoll Separating Solution 40 %, density 1.055g/ml at +20°C 100ml
L 6173 Percoll Separating Solution 80 %, density 1.02g/ml at +20°C 100ml
M 5030 Phytohemagglutinine PHA L 1x(6x5ml)
M 5040 Phytohemagglutinine PHA E 1x(6x5ml)
M 5050 Concanavalin A (Con A) 1x(6x5ml)
M 5060 Pokeweed mitogen (PWM) 1x(6x5ml)

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Antibiotics

 

Cell culture media provide an ideal environment also for rods such as bacteria and fungi. Basically, cell cultures can be protected against contamination by strict adherence to sterile technique; but only the discovery of antibiotics made advanced cell culture

possible. However, as antibiotics have a toxic potental, their dosage must closely be monitored.

Ordering Information
Cat.No Product Units
A 2610 Amphotericin B, 250 µg/ml, lyophilized 1 x (6 x 5ml)
A 2612 Amphotericin B, 250 µg/ml 50ml
A 2812 Partricin 50ml
A 2710 Gentamycin 10 mg/ml, lyophilized 1 x (6 x 5ml)
A 2712 Gentamycin 10 mg/ml 50ml
A 271-23 Gentamycin-Sulfate 2.5g
A 271-25 Gentamycin-Sulfate 10g
A 271-26 Gentamycin-Sulfate 25g
A 2512 Kanamycin 5 mg/ml 50ml
A 231-40 Polymyxin B-Sulfate, approx. 6.000 - 8.000 U/mg 5 Mio IE
A 2412 Neomycin/Bacitracin 50 U/ml 50ml
A 2210 Penicillin/Streptomycin 10.000 U/10.000 µg/ml, lyophilized 1 x (6 x 5ml)
A 2212 Penicillin/Streptomycin 10.000 U/10.000 µg/ml 50ml
A 2213 Penicillin/Streptomycin 10.000 U/10.000 µg/ml 100ml
A 321-42 Penicillin 25 Mio IE
A 321-44 Penicillin 100 Mio IE
A 331-26 Streptomycin –Sulfate 25g
A 331-27 Streptomycin –Sulfate 100g

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Balanced Salts & Buffers

 

 
Ordering Information
Cat.No Product Units
L 6015 Alsever Solution pH 6.1 500ml
L 1913 Earle's Saline Solution (EBSS) with 2.2g/l NaHCO3 100ml
L 1915 Earle's Saline Solution (EBSS) with 2.2g/l NaHCO3 500ml
L 1925 Earle's Saline Solution (EBSS) (10X) without NaHCO3 500ml
L 2013 Hanks' Saline Solution (HBSS) with 0.35g/l NaHCO3 100ml
L 2015 Hanks' Saline Solution (HBSS) with 0.35g/l NaHCO3 500ml
L 2025 Hanks' Saline Solution (HBSS) (10X) without NaHCO3 500ml
L 2035 Hanks' Saline Solution (HBSS) without phenol red, with 0.35g/l NaHCO3 500ml
L 2045 Hanks' Saline Solution (HBSS) w/o Ca2+, Mg2+,w/o phenol red,w.0.35g/l NaHCO3 500ml
L 2053 Hanks' Saline Solution (HBSS) w/o Ca2+, Mg2+,w. phenol red, w.0.35g/l NaHCO3 100ml
L 2055 Hanks' Saline Solution (HBSS) w/o Ca2+, Mg2+, w. phenol red,w.0.35g/l NaHCO3 500ml
L 201-01 Hanks' Salts powder substance without NaHCO3 1litre
L 201-05 Hanks' Salts powder substance without NaHCO3 5litres
L 201-10 Hanks' Salts powder substance without NaHCO3 10litres
L 201-50 Hanks' Salts powder substance without NaHCO3 50litres
L 1613 HEPES buffer solution (1M) (50X) 100ml
L 1603 HEPES powder substance 100g
L 1713 Sodium bicarbonate (7.5%) NaHCO3 100ml
L 1703 Sodium bicarbonate NaHCO3 powder substance 100g
L 1815 PBS Solution with Ca2+, Mg2+ 500ml
L 1823 PBS Solution without Ca2+, Mg2+ 100ml
L 1825 PBS Solution without Ca2+, Mg2+ 500ml
L 1835 PBS Solution (10X) without Ca2+, Mg2+ 500ml
L 182-01 PBS-powder substance without Ca2+, Mg2+ 1litre
L 182-05 PBS-powder substance without Ca2+, Mg2+ 5litres
L 182-10 PBS-powder substance without Ca2+, Mg2+ 10litres
L 182-50 PBS-powder substance without Ca2+, Mg2+ 50litres
L 0015 Ultra Pure Water in glass bottle, sterile 500ml
L 0020 Ultra Pure Water, sterile, in Duran bottles suitable for HPLC 1litre
L 0030 Ultra Pure Water, not sterile 5litres
L 0040 Ultra Pure Water, not sterile 10litres

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Cell Culture Reagents

 

Ordering Information
Cat.No Product Units
L 6510 Heparin stabilizer free 5000U/ml lyophilized (for 5ml) 1 x (6 x 5ml)
L 0473 Sodium pyruvate 100mM 100ml
K 0293 Non-essential amino acids (NEA) (100X) 100ml
K 0363 MEM Amino Acids (50X) 100ml
K 0373 MEM Vitamins (100X) 100ml
K 0280 L-Glutamine (200mM) 1 x (24 x 2.5ml)
K 0281 L-Glutamine (200mM), lyophilized 1 x (6 x 5ml)
K 0282 L-Glutamine (200mM) in plastic bottles 50ml
K 0202 N-Acetyl-L-alanyl-L-glutamine, 200mM (stable glutamine) 50ml
L 3010 Biotect Protection Medium 24 x 2.5ml
L 6413 KCI solution (hypotonic) 0.075M 100ml

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Cell Culture Diagnostics

 

Ordering Information
Cat.No Product Units
F 0483 HAT-Supplement (50X) 100ml
F 0493 HT-Supplement (50X) 100ml
D 7001 Mycoplasma test - DNA-staining kit 1Kit
L 2203 Phenol red 0.5 % 100ml
L 6313 Neutral red (1:300 - 0.3%) 100ml
L 6323 Trypan blue 05+A2% in phys. Saline Solution 100ml

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Copyright © 2002 GENTAUR Molecular Products
Last modified: 05/29/09