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Signal Enhancer HIKARI

 

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Signal Enhancer HIKARI


 
Dilute your antibodies with Signal Enhancer HIKARI instead of conventional diluents such as PBS or TBS before performing your next Western blot detection protocol and witness a remarkable increase in the ability to detect the protein of interest and to eliminate undesired background.
Signal Enhancer HIKARI was developed to resolve the problems of low sensitivity and high background often encountered during procedures such as Western blotting and ELISA.



Features

Enhances antigen-antibody reaction: yields several- to several 10-fold increase in signal intensity
Easily removes background: improves the specificity of your antibodies
Works with any substrate: enhances both chemiluminescence and colorimetric detection
Works with any membrane: enhances signal from nitrocellulose and PVDF membrane
Ready-to-use reagent: just dilutes your antibodies with Signal Enhancer HIKARI



Application 1

Detection enhancement of Rac1 with treatment of HIKARI in Western blotting
 
Treatment with Signal Enhancer HIKARI resulted in clear signal, whereas dilution with conventional PBS-T resulted in no signal.

 


Primary Ab: Anti-Rac1 (1: 1,000)
 
Secondary Ab: Anti-Mouse IgG-HRP (1: 2,500) Sample: Rat cortical primary neuron
Detection: ECL

 

 
Data courtesy of Dr. K. Ishizuka,
Department of Neuroscience,
 
Johns Hopkins University School of Medicine


Application 2
 
Detection enhancement of pJNK with treatment of HIKARI in Western blotting
Treatment with Signal Enhancer HIKARI resulted in clear signal enhancement and background suppression compared with the conventional method using TBS-T.

 


Primary Ab: Anti-pJNK  (1: 1,000)
 
Secondary Ab: Anti-rabbit IgG - HRP (1: 5,000)
 
Sample: Mouse Embryonic Fibroblast (MEF)
 
Detection: SuperSignal West Pico

Data courtesy of Dr. S.
Matsuzawa,
 
Signal Transduction, NCI Cancer Center,
 
Burnham Institute for Medical Research



Technical information

Signal Enhancer HIKARI Procedure (PDF)



Ordering information
 

Product name
 
Kit Contents Grade Storage Code No.
 
PKG Size
Signal Enhancer HIKARI
 
Solution 1 for Primary antibodies
Solution 2 for secondary antibodies
SP Refrigerator 08043-81 1 Set
(50ml each)
08044-71 1 Set
(250ml each)

 

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Last modified: 05/29/09