IMPORTANT : use cold 0.5M Acetic Acid instead of pure water in the reconstitution (the final pH of the protein solution should be 6.0)
Recombinant Human Interleukin-6 (IL6)
IL-6 (Human) 5.0 x 107 IU/mg
04-C009A 50μg 542 €
04-C009B 1mg 2.765 €
Latest batch Expiry date 15/12/2009
Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino
acid residues that contain four conserved cysteine residues involved in two disulphide bonds.
They have a compact, globular fold (similar to other interleukins), stabilized by the 2
disulphide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a
left-handed twist: the helices are anti-parallel, with 2 overhand connections, which fall into a
2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological
activity of the molecule.
Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine
expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage
and to promote the proliferation of advanced stage melanoma cells in vitro.
Recombinant Human IL-6 produced inE. coli is a single, non-glycosylated polypeptide
chain containing 185 amino acids and having a molecular mass of 21.0 kDa.
Biological activity:rHuIL-6 is fully biologically active when compared to standard. The
ED50 as determined by the dose-dependant stimulation of human TF-1 cells is less then 0.1
ng/ml, corresponding to a Specific Activity of 5.0 x 107 IU/mg.
Purity:Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Anion-exchange FPLC.
(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
Molecular weight:21 KD+/-10% determined by reduced SDS-PAGE.
Amino-Acid Sequence:The sequence of the first five N-terminal amino acids was
determined and was found to be Ser-Pro-Val-Pro-Pro.
Endotoxin:Less than 0.3ng/μg (0.3IEU/μg) determined by LAL test.
Formulation:Lyophilized from a concentrated (0.5mg/ml) solution in water containing
20mg/ml sodium acetic.
Storage:Lyophilized rHuIL-6 although stable at room temperature for 3 weeks, should be
stored desiccated below -18°C. Upon reconstitution rHuIL-6 should be stored at 4°C between
2-7 days and for future use below -18°C. For long-term storage it is recommended to add a
carrier protein (0.1% HSA or BSA).
Please avoid freeze-thaw cycles.
Reconstitution:It is recommended to reconstitute the lyophilized rHuIL-6 in 500mM acetic
acid not less than 100μg/ml, which can then be further diluted to other aqueous solutions.
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