Home Up Feedback Contents Search Sell your Prod. Meet us News           

 
Home • Up

human IL-6

 

Home

GENTAUR

+32 1658 9045

or

0032 (0)16 41 44 07

+32 1650 9045

info@gentaur.com

Av. de l' Armée 68

B-1040 Brussels

BELGIUM

France

tel 01 43 25 01 50

fax01 43 25 01 60

9, rue Lagrange

75005 Paris

Italia

tel 02 36 00 65 93

fax 02 36 00 65 94

20135 Milano

Deutschland

tel +32 1658 9045

fax +32 1650 9045

Polska

Tel 058 710 33 44

Fax 00 32 16 50 90 45

ul. Grunwaldzka 88A/2

81-771 Sopot

日本

tel +81 78 386 0860

fax +81 78 306 0296

Minaatojimaminami-manchi

Chuo-ku, Kobe

065-0047

Österreich
+43720880899

Canada Montreal
+15149077481

Česká republika Praha
+420246019719

Danmark
+4569918806

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494

Magyarország Budapest
+3619980547

Ireland Dublin
+35316526556

Luxembourg
+35220880274

Nederland
+31208080893

Norge Oslo
+4721031366

Polska Warszawa
+48223988221

Sverige Stockholm
+46852503438

Schweiz Züri
+41435006251

US New York
+17185132983

Other Countries
0032 (0)16 41 44 07


 

 

 

 

 

Clonagen

Gentaur

Genprice

Bioxys

Labprice

 IMPORTANT : use cold 0.5M Acetic Acid instead of pure water in the reconstitution (the final pH of the protein solution should be 6.0)

Recombinant Human Interleukin-6 (IL6)  

IL-6 (Human) 5.0 x 107 IU/mg

04-C009A 50μg 542 €

04-C009B 1mg 2.765 €
04-C009C 5mg 8.514 €

Latest batch Expiry date 15/12/2009

Background:

Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino

acid residues that contain four conserved cysteine residues involved in two disulphide bonds.

They have a compact, globular fold (similar to other interleukins), stabilized by the 2

disulphide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a

left-handed twist: the helices are anti-parallel, with 2 overhand connections, which fall into a

2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological

activity of the molecule.

Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine

expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage

and to promote the proliferation of advanced stage melanoma cells in vitro.

Description:

Recombinant Human IL-6 produced in E. coli is a single, non-glycosylated polypeptide

chain containing 185 amino acids and having a molecular mass of 21.0 kDa.

Quality Control:

Biological activity: rHuIL-6 is fully biologically active when compared to standard. The

ED50 as determined by the dose-dependant stimulation of human TF-1 cells is less then 0.1

ng/ml, corresponding to a Specific Activity of 5.0 x 107 IU/mg.

Purity: Greater than 98.0% as determined by:

(a) Analysis by RP-HPLC.

(b) Anion-exchange FPLC.

(c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Molecular weight: 21 KD+/-10% determined by reduced SDS-PAGE.

Amino-Acid Sequence: The sequence of the first five N-terminal amino acids was

determined and was found to be Ser-Pro-Val-Pro-Pro.

Endotoxin: Less than 0.3ng/μg (0.3IEU/μg) determined by LAL test.

Formulation: Lyophilized from a concentrated (0.5mg/ml) solution in water containing

20mg/ml sodium acetic.

Storage: Lyophilized rHuIL-6 although stable at room temperature for 3 weeks, should be

stored desiccated below -18°C. Upon reconstitution rHuIL-6 should be stored at 4°C between

2-7 days and for future use below -18°C. For long-term storage it is recommended to add a

carrier protein (0.1% HSA or BSA).

Please avoid freeze-thaw cycles.

Reconstitution: It is recommended to reconstitute the lyophilized rHuIL-6 in 500mM acetic

acid not less than 100μg/ml, which can then be further diluted to other aqueous solutions.

 

Send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2002 GENTAUR Molecular Products
Last modified: 05/29/09