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Sulfonamides Elisa

 

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Clonagen

Gentaur

Genprice

Bioxys

Labprice

Sulfonamides residues (Five) ELISA Test Kit

                        Price: € 361          art.code: 202-SULFR-E

1. Principle

This test  kit is based on the competitive enzyme immunoassay for the detection of Sulfamonomethoxine(SMM), Sulfadiazine(SD), Sulfamerazine(SM1), Sulfamethoxazole(SMZ) and Sulfathiazole(ST) in the tissue(chicken, pork), milk, honeyand egg. The conjugate antigen is pre-coated on the micro-well stripes. The Sulfonamides residues in the testing sample competes with the conjugate antigen pre-coated on the micro-well stripes, to interact with the antibody against Sulfonamides. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Sulfonamides residues in the sample. This value is compared to the standard curve and the content of the corresponding Sulfonamides residues is subsequently obtained.

 

2. Technical specifications

Sensitivity : 1ppb

Detection limit:

Meat(chicken,pork), liver(chicken,pork),honey, egg  ——1ppb

Serum, urine ——————————————————— 4ppb

Milk ——————————————————————— 20ppb

Recovery rate

Meat(chicken,pork), liver(chicken,pork)  ————— 70 ±10%

Egg  ————————————————————  65 ±10%

Milk ,honey,serum  —————————————— 70 ±10%

Cross-reaction rate:

Sulfadiazine(SD)  ——————————————— 100%

Sulfamonomethoxine(SMM)  —————————— 97.6%

Sulfamerazine(SM1) —————————————— 12%

Sulfamethoxazole(SMZ)  ———————————  63%

Sulfathiazole(ST) ——————————————— 195%

 

This test kit is used to detect 4 Sulfonamides based on cross-reaction rate, its detection limit as follows: SD—1ppb  SMM— 1ppb  SMZ —2ppb  ST —0.5ppb  SM1—10ppb

 

 

96 wells /kit

 

 

 

 

 

 

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Last modified: 05/29/09