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Total RNA Extraction Kits

 

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Total RNA Extraction Kits

The series of Total RNA Extraction Kits are specially designed for purification of total RNA from a variety of samples, such as bacteria, yeasts, plant tissues, animal tissues, cultured cells, fresh human whole blood, etc. The method uses detergents and a chaotropic salt to lyse cells and inactivate RNase, then RNA in chaotropic salt solutions binds to the glass fiber matrix in the columns.

 

 

After washing off the contaminants, purified RNA is eluted by RNase-free water. The entire procedure can be completed within 1 hour and the purified RNA is ready for plenty of applications.


 

Advantages

RT-PCR
cDNA synthesis
In vitro translation
Primer extension
Northern blotting
cDNA library construction


 

Storage Conditions

Store at room temperature (15 ~ 25°C)


 

Quality Control

The quality of Total RNA Extraction Kits is tested on a lot-to-lot basis.


 

Note

For research use only. Not for use in diagnostic or therapeutic procedures.
RB Buffer contains chaotropic salt which is a harmful and irritant agent. During operation, always wear a lab coat, disposable gloves, and protective goggles.


 

Use Conditions

Sample Source: animal tissue, whole blood/buffy coat, cultured animal cells, gram+/– bacteria
Sample Size: 30 mg for tissue, 107 animal cells, 300 μl of whole blood, 109 bacterial cells
Preparation Time: 20 min


 

References

Vogelstein, B., and Gillespie, D. (1979) Proc. Natl. Acad. Sci. USA 76: 615-619.


 

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Copyright © 2002 GENTAUR Molecular Products
Last modified: 05/29/09