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Clonagen

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TrueVision is a unique and highly effective reagent that dramatically improves the sensitivity and
signal-to-noise ratio of immunohistochemical staining, allowing you to clearly visualize cellular targets
that would normally indistinguishable due to low level target expressions or high background
staining.

Highlights:

Enhance your staining detection signal
Boost your immunohistochemical staining
signal up to 10-fold.

Promote antibody-antigen interaction
Work through a novel mechanism by
increasing antigen accessibility in
immunohistochemical staining

Easy to use
Ready to use reagent and no need to change
your current protocol. Simply dilute your
antibodies with TrueVision reagent.

 

Product:

Each bottle contains 20ml of the reagent. Store at
4° C and stable for three months from the date of
shipment.

 

Protocol:

1. Sample preparation
    a. Tissue culture cells are grown on glass slides and fixed with acetone or others.
    b. Frozen tissue blocks are sliced 4 to 10 micron thick. The sections are adhered to glass slides and
        fixed with acetone or others
        (Optional: Incubate for 5–10 minutes in 0.1–1% hydrogen peroxide in PBS to quench endogenous
        peroxidase activity)

2. Immunohistochemical staining
    a. Block the samples with your blocking reagents
    b. Incubate the samples with primary antibodies diluted in TrueVision reagent for 60 min,
        wash with TBS
    c. Incubate the samples with fluorescence conjugated secondary antibodies diluted in
        TrueVision reagent for 60min. Wash with TBS
        Or
        Incubate the samples with HRP conjugated secondary antibodies diluted in TrueVision
        reagent for 60 min, wash with TBS
    d. If HRP conjugated secondary antibodies are used, apply DAB for 5 to 30min staining. Wash the
        samples with TBS and counterstain.
    e. Mount

 

Related products:


Blocking Reagent         siRNA cloning kit
Luminol Reagent          mRNA detection kit
siRNA products            Chemokine products

 

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Last modified: 05/29/09