Home Up Feedback Contents Search Sell your Prod. Meet us News           

 
Home • Up

Velocity

 

Home

GENTAUR

+32 1658 9045

or

0032 (0)16 41 44 07

+32 1650 9045

info@gentaur.com

Av. de l' Armée 68

B-1040 Brussels

BELGIUM

France

tel 01 43 25 01 50

fax01 43 25 01 60

9, rue Lagrange

75005 Paris

Italia

tel 02 36 00 65 93

fax 02 36 00 65 94

20135 Milano

Deutschland

tel +32 1658 9045

fax +32 1650 9045

Polska

Tel 058 710 33 44

Fax 00 32 16 50 90 45

ul. Grunwaldzka 88A/2

81-771 Sopot

日本

tel +81 78 386 0860

fax +81 78 306 0296

Minaatojimaminami-manchi

Chuo-ku, Kobe

065-0047

Österreich
+43720880899

Canada Montreal
+15149077481

Česká republika Praha
+420246019719

Danmark
+4569918806

Finland Helsset
+358942419041

Ελλάς Αθήνα
+302111768494

Magyarország Budapest
+3619980547

Ireland Dublin
+35316526556

Luxembourg
+35220880274

Nederland
+31208080893

Norge Oslo
+4721031366

Polska Warszawa
+48223988221

Sverige Stockholm
+46852503438

Schweiz Züri
+41435006251

US New York
+17185132983

Other Countries
0032 (0)16 41 44 07


 

 

 

 

 

Clonagen

Gentaur

Genprice

Bioxys

Labprice

Features
 
  • High-speed, high fidelity DNA polymerase
     
  • High processivity
     
  • Fast amplification
     
  • Shorter PCR runs for longer templates
     
  • Robust, requires minimal optimization of the reaction
     
Applications
 
  • Cloning techniques where high fidelity is desirable
     
  • Blunt-end cloning
     
  • Amplification of difficult templates
     
  • Mutagenesis
     
Description

VELOCITY™ is a fast thermostable enzyme possessing 5’-3’ DNA polymerase and 3’-5’ proofreading exonuclease activities. VELOCITY provides high fidelity with an error-rate of 8 x 10-7 combined with a high speed DNA synthesis as fast as 15s/Kb for templates of up to 5Kb and 30s/Kb for templates longer than 5Kb. The inherent high speed DNA synthesis is due to the enhanced processivity of the polymerase.

VELOCITY is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons. The error rate of VELOCITY DNA Polymerase (8 x 10-7) was determined using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999).

Two buffers are provided with VELOCITY DNA Polymerase. For general high fidelity applications, use 5x Hi Fi Reaction Buffer for GC-Rich or difficult templates, use 5x GC Reaction Buffer.

 
Unit Definition

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.


 
Concentration

2u/µl


 
Storage Conditions

VELOCITY DNA Polymerase can be stored for 12 months at -20°C.


 
Shipping Conditions

On Dry Ice or Blue Ice

 

Price:

BIO-21098 250 Units € 195
BIO-21099 500 Units € 360

 

 

 

Send mail to webmaster@gentaur.com with questions or comments about this web site.
Copyright © 2002 GENTAUR Molecular Products
Last modified: 05/29/09