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YB Plasmid Extraction Kits

 

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YB Plasmid Extraction Kits (download PDF)

YB Plasmid Extraction Kits are designed for rapid isolation of plasmid or cosmid DNA from bacterial cultures. In the process, the modified alkaline lysis method and RNase treatment are used to get clear cell lysate with minimal genomic DNA and RNA contaminants. In the presence of a chaotropic salt, the plasmid DNA in the lysate binds to glass fiber matrix in the column.

 

 

The contaminants are washed with ethanol wash buffer. In the final step, the purified plasmid DNA is eluted by low salt elution buffer or distilled water. The protocol does not require phenol extraction or alcohol precipitation. The entire procedure can be completed in 20 minutes and the purified plasmid DNA is ready for various applications.


 

Applications

Plasmid DNA preparation
DNA sequencing
DNA library screening and analysis
Ligation and transformation
Transfection


 

Storage Conditions

Store at room temperature (15 ~ 25°C)


 

Quality Control

The quality of YB Plasmid Extraction Kits is tested on a lot-to-lot basis.


 

Note

For research use only. Not for use in diagnostic or therapeutic procedures.
Buffer contains guanidine hydrochloride which is a harmful and irritant agent. During operation, always wear a lab coat, disposable gloves, and a protective goggle.


 

References

Birnboim, H. C., and Doly, J. (1979) Nucleic Acids Res. 7: 1513-1523.
Vogelstein, B., and Gillespie, D. (1979) Proc. Natl. Acad. Sci. USA 76: 615-619.

 

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Last modified: 05/29/09