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YB Rapid Ligation Kit

 

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YB Rapid Ligation Kit

Introduction

YEA T4 DNA ligase catalyzes the joining of two strands of DNA between the 5’ – phosphate and the 3’ – hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended termini. YB rapid DNA ligation kit is designed for efficient ligation of DNA inserts into plasmid vectors in just 5 minutes.

 

 

 


 

Features & Benefits

Fast 
Ligation can be achieved within 5 minutes
Time-Saving
The combination of YB rapid ligation kit and ECOSTM competent cell allow the whole process to be finished within 6 minutes
High efficiency
The combination of YB rapid ligation and ECOSTM also brings you high efficiency
Economic
YB rapid ligation kit is better in quality and cheaper in price


 

Applications

 Join double-stranded DNA with cohesive or blunt termini
 Join oligonucleotide linkers to DNA sequence with blunt end
 Repair nicks in duplex DNA, RNA, or DNA-RNA hybrids


 

Components

Storage Buffer
20 mM Tris-HCl (pH7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, and 50% glycerol stabilizers
10× Ligation Buffer A
0.4 M Tris-HCl, 0.1 mM MgCl2, 0.1 M DTT, and 5 mM ATP (pH5.0 at 25°C) The performance of this buffer depends on the integrity of the ATP. Store the buffer in small aliquots at -20°C to minimize degradation of the ATP and DTT.
10× Ligation Buffer B
Buffer contains an enhancer which dramatically increases ligation efficiency for blunt end DNA.


 

Storage Conditions

Store at –20°C


 

Ordering Information

 

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Last modified: 05/29/09