Antibiotics ELISA

Florfenicol , also called fluorine Thiamphenicol (Florfenicol, FF), which is the derivatives of Thiamphenicol (TAP). Florfenicol is widely used to treat livestock, fowl, aquaculture and bee product for its obvious function on various gram-positive, gram-negative bacteria , mycoplasma. But its residues in food easily cause the latent aplastic anemia,so the United Nations Code of Food Statute Committee (CAC) and many countries stipulated that Florfenicol (FF) residues in food must be below 0.1ppm, Japanese detection limit is below 0.1ppm, Chinese detection limit is 1ppm. Florfenicol is detemined most commonly by HPLC or LC/MS before, this method has disadvantages regarding sample pre-processing , laboratory practice, time and cost requirement.

Our company scientific researchers make every industrious efforts to overcome the technical difficulty for a nearly year, and finally develop Florfenicol ELISA fast test kit in the world firstly, which achieves the international advanced standard; at the same time ,its determination result is consistent with HPLC or the LC/MS. Compared with HPLC or LC/MS , Florfenicol ELISA fast test kit of Green Spring brand shows advantages regarding sensitivity , specificity , accurate determination of quota based on enzyme inmmunoassay. It is possible to be used in massive samples fast screening and detect Florfenicol residues in the animal tissue (muscle, liver), urine, serum, honey, intestine, milk as well as aquatic product ,etc.

Specification: 96 wells/kit

Benzyl penicillin is widely applicable antibiotic, which is commonly employed in prevention, therapy, diagnosis of animal disease, or adjustment of physiological functions. Benzyl penicillin is synthesized to control animal disease effectively and promote the healthy development of farming industry,.If animals absorb it too much, will cause Bp-residues in animal food. Mankind produces allergic reaction and tolerance to a drug resulting from taking high residues food. Use of Benzyl penicillin was resitricted in the EU and America. The EU regulated that maximum residue in milk is 4μg/kg, Chinese Agriculture Ministry made corresponding national policies restrict its application.

Compared withLC-UV, LC-MS and LC-MS/MS , Benzyl penicillin (Bp) ELISA Test Kit shows considerable advantages regarding testing process,sensivity,technical equipment and time requirement.
Competetive enzyme immunoassay for the quantitative analysis of benzyl penicillin in honey, milk, fish, shrimp, muscle and liver.

All reagents required for the enzyme immunoassay-including stands-are contained in the test kits.
The test kit is sufficient for 96 determinations(including stands)

Specification:96 wells/kit
Sensitivity: 0.1 ppb
Test linear dimensional : 0.1--8.1ppb

Sample preparation time:
honey and milk …………………………… 10min
fish, shrimp, muscle and liver …………15min

Detection limit:
honey and milk ………………………… 2ppb
fish, shrimp, muscle and liver ……… 2ppb

Recovery rate:
fish, shrimp, muscle and liver……… 75-95%
honey and milk …………………………… 60-80%

Cross-reactions:
Benzylpenicillin ………………………… 100%
Ampicillin ………………………………… 0.8%
Cloxacillin …………………………………0.2%
Dicloxacillin……………………………… 0.1%
Amoxicillin………………………………… 0.1%
Ceftiofur ……………………………………0.1%

Test implemention time: 2 h

Specification: 96 wells/kit
Sensitivity: 0.5 ppb
Test linear dimensional : 0.5--40.5ppb

Detection limit:
muscle……………………………1ppb
liver ……………………………2ppb
urine ……………………………1ppb
admixture ………………………25ppb
contrated material ,premix material ………………75ppb

Sample preparation time:
urine …………………………… 10min
muscle and liver…………………20min
feed …………………………… 30min

Recovery rate：
urine……………………………75-95%
muscle and liver………………70-90%

Cross-reactions:
Ractopamine…………………………… 100%
Dobutamine…………………………approx13%
Clenbuterol ……………………………〈0.1%
Salbutamol ……………………………〈0.1%

Test implemention time: 80 min

Enrofloxacin is quinolones antibiotics, which is used in veterinary diagnosis and aquiculture to improve growth speed, pevent and treat disease as important drug against infection for its inhibition bacteria DNA gyrase, broad antibacteria, high efficiency, low toxicity .

There are spectrophotometry ,liquid chromatography and ELISA to detect Enrofloxacin. ELISA test is considered to be common method for its high sensitivity and convenient implementation
Enrofloxacin ELISA Test Kit based on competitive enzyme immunoassay for the quantitative analysis of enrofloxacin in fish,shrimp, honey, serum, tissue and liver (chicken, pork), etc.
All reagents required for the enzyme immunoassay-including stands-are contained in the test kit.
The test kit is sufficient for 96 determinations(including stands)

Specification: 96wells/kit
Sensitivity: 0.5 ppb
Test linear dimensional: 0.5--40.5ppb

Detection limit:
serum …………………………… 1ppb
honey …………………………… 2ppb
tissue( fish, shrimp, meat and liver ) ………………1ppb

Sample preparation time:
pork ,chicken …………………… 50min
finsh, shrimp …………………… 20min
serum,honey …………………… 10min

Recovery rate：
serum …………………………… 65-95%
honey ……………………………60-90%
fish, shrimp……………………… 60-90%
meat and liver…………………… 60-90%

Cross-reactions:
Enrofloxacin……………………… 100%
Ciprofloxacin …………………… 1.5%
Norfloxacin ……………………… 1.8%

Test implemention time: 90 min

Furazolidone is synthetic broad-spectrum antibiotic and can treat animal desease , which is frequently employed in animal and aquaculture production. But Furazolidone and its metabolite residues in animal food are passed to human through the food chain ,regularly taking it them will cause various diseases, which can lead to cancer, teratogenesis and other side effects . Furazolidone is was prohibited from use in food animal production in the EU in 1995 ,and strictly implemented detection of Furazolidone, along with this America formulated the corresponding policy and regulations in 2002. The use of Nitrofurantoin was classified as forbidden drugs in Veterinary Medicine and other Compounds banned in Food Animal Lists which is pulished by Chinese Agriculture Ministry in March, 2002.
Compared with chromatographic method, AOZ ELISA Test Kit shows considerable advantages regarding sensivity,detection limit, technical equipment and time requirement.
Competitive enzyme immunoassay for the quantitative analysis of AOZ in fish, shrimps, honey, meat (chicken, pork, beef) and liver.
All reagents required for the enzyme immunoassay-including stands-are contained in the test kit.
The test kit is sufficient for 96 determinations(including stands)

Specifications: 96 wells/kit
Sensitivity: 0.05 ppb
Test linear dimensional: 0.05--4.05ppb

Detection limit:
honey, meat and live………………………0.1ppb
fish and shrimp ……………………………0.2ppb

Sample preparation time:
fish and shrimp ……………………………16h
honey,meat and liver …………………… 16h

Recovery rate：
fish and shrimp …………………………90-100%
honey,meat and liver ……………………60-90%

Cross-reations:
AOZ ……………………………………… 100%
AMOZ…………………………………… ﹤0.1%
AHD ………………………………………﹤0.1%
SEM …………………………………… ﹤0.1%

Test implemention time: 90 min

Nitrofurans are synthetic broad-spectrum antibiotics,which are frequently employed in animal production for its excellent antibacterial and pharmacokinetic properties.they had been also used as growth promoters in the pig production, poulty and fish sector. In long term studies with experimental animals, the parent drugs and their metabolites showed carcinogenic and mutagenic characteristics.This has led to a prohibition of naturfurans for the treatment used for food production.The naturafuran drugs furantadone,nitrofurantion and nitrofurazone were banned from use in food animal production in the EU in 1993, and the use furazolidone was prohibited in 1995.
The analysis of residues of nitrofuran drugs needs to be based on the detection of the tissue bound matabolites of the nitrofuran parent drugs.Science the parent drugs are very rapidly metabolized, they are not detectable for a short time after treatment. The tissue bound nitrofuran metabolites are detectable for a long time after administration and therefore they are used for the detection of the abuse of nitrofurans. Nitrofuran metabolites are found after administration of Furanltadone(AMOZ), Furazolidone(AOZ), Nitrofurazone(SEM), Nitrofurantoin (AHD).
AMOZ-residues are determined most commonly by LC-UV,LC-MS techniques. Enzyme immunoassay,compared with chromatographic methods, show considerable advantages regarding sensitivity, detection limit, technical equipment and time requirement.
Using the RIDSCREEN Nitrofuran (AMOZ) test, it is possible to detect AMOZ in fish, shrimps, honey, meat (chicken,pork,beef) and liver.
All reagents required for the enzyme immunoassay-including stands-are contained in the test kits.
The test kit is sufficient for 96 determinations(including stands)

Specifications:96 wells/kit
Sensitivity: 0.1 ppb
Test linear dimensional: 0.1--8.1ppb

Detection limit:
honey, meat and liver……………………… 0.2ppb
fish and shrimp …………………………… 0.3ppb

Sample preparation time:
fish and shrimp ……………………………2h
honey,meat and liver ………………………2h
Recovery rate：
fish and shrimp ……………………………75-105%
honey,meat and liver……………………… 65-95%

Cross-reations:
AMOZ ……………………………………… 100%
AOZ ……………………………………… ﹤0.1%
AHD ……………………………………… ﹤0.1%
SEM ……………………………………… ﹤0.1%

Sulfaquinoxaline（SQX）ELISA Test Kit

Price: € 322 (art.code: 220-SQX-E)

Sulfaquinoxaline is widespread use of antibiotics, which has considerable effect on controling and treating animal disease. Sulfaquinoxaline in hunman body causes tolerance of drug and result in potential cancer for its serious side effect.

Competitive enzyme immunoassay shows considerable advantages regarding sensitivity, detection limit, technical equipment and time requirement; SQX ELISA test kit is possible to detect sulfaquinoxaline in urine, serum, honey, milk, whole egg, muscle and liver.
All reagents required for the enzyme immunoassay-including stands-are contained in the test kits.
The test kit is sufficient for 96 determinations(including stands)

Specification: 96 wells/kit
Sensitivity: 1 ppb
Test linear dimensional: 1--81ppb

Detection limit:
egg, muscle and liver ……………………… 1ppb
urine and serum …………………………… 4ppb
honey ……………………………………… 1ppb

Sample preparation time:
Pork,chicken,egg ……………………………20min
honey, milk and serum………………………10min

Recovery rate:
honey, milk and serum ……………………60-80%
muscle and liver ……………………………60-80%
egg ………………………………………… 60-75%

Test implemention time: 1.5 h

Clenbuterol belongs to beta stimulant, and is high selective stimulant and hormone, which is widely employed in cure of bronchial asthma,chronic bronchitis, pulmonary emphysema, and can increase muscle , reduce fat sediment, promote animal growth. It is frequently added in feed to improve animal growth. Because its input quantity is larger than treatment quantity, Clenbuterol residue in animals brings serious danger to customers. No institution approves that Clenbuterol is used to promote the animal growth.
Clenbuterol ELISA test kit has considerable advantages regarding sensitivity, detection limit, technical equipment and time requirement based on competitive enzyme immunoassay, it is possible to detect Clenbuterol in urine, serum, feed, muscle and liver.
All reagents required for the enzyme immunoassay-including stands-are contained in the test kits.
The test kit is sufficient for 96 determinations(including stands)

Specification: 96 wells/kit
Sensitivity: 0.1 ppb
Test linear dimensional: 0.1—4.05ppb

Detection limit:
urine and serum……………………………0.1ppb
muscle and liver ……………………………0.1ppb
feed …………………………………………10 ppb

Sample preparation time:
urine and serum ………………………… 10min
muscle and liver ………………………… 30min
feed………………………………………… 30min

Recovery rate:
urine and serum ………………………… 80-100%
muscle and liver ………………………… 70-90%
feed ……………………………………… 65-95%

Cross-reactions:
Clenbuterol ………………………………… 100%
Salbutamal ………………………………… 21.5%
Terbutalin ………………………………… ﹤1%

Ractopamine ……………………………… ﹤1%

Test implemention time: 80 min

Sulfanethazine (SM2) is wide use antibiotic, which has remarkable effect on controlling and curing livestock and poultry diseases. But it is easy to remain in animal body; also has considerable side effect on human, which can cause faint, headache, exhaust, nausea, vomit,etc; lead to hemolytic anemia, a vacuity of granulocyte, allergic reaction and damage urinary and nervous system; cause the increasing tolerance of drugs. It was regulated that maximum Sulfanethazine residue limit in animal food and feed is 100ppb in America, China, the EU.
Sulfanethazine (SM2) is determined by enzyme immunoassay, shows considerable advantages regarding sensitivity,detection limit, technical equipment and time requirement. It is possible to detect sulfamethazine in egg, honey, milk, urine, serum, muscle and liver.
All reagents required for the enzyme immunoassay-including stands-are contained in the test kits.
The test kit is sufficient for 96 determinations(including stands)
Specification:96 wells/kit
Sensitivity: 1 ppb
Test linear dimensional: 1—81 ppb

Detection limit:
milk …………………………………………20ppb
urine and serum ……………………………4ppb
honey, egg, muscle and liver……………1ppb

Sample preparation time:
Honey,milk,urine,serum………………… 10min
egg ………………………………………… 20min
muscle and liver………………………… 20min

Recovery rate：
muscle and liver …………………………60-80%
honey,milk and serum ……………………60-80%
egg ………………………………………… 55-75%

Test implemention time: 80 min

Chloramphenicol（CAP）ELISA Test Kit

Price: € 231 (art.code: 220-CAP-E)

Chloramphenicol is broad-spectrum antibiotic,which is frequently employed in livestock, poultry, aquatic food and honey product to treat various infectious diseases for its excellent in effect and price. It can restrain blood-forming of bone marrow and lead to aplastic anemia, granulocytes deficiency. Chloramphenicol was banned from use food animal production in many countries.
Chloramphenicol ELISA test kit based on Competitive enzyme immunoassay shows considerable advantages regarding sensitivity,detection limit, technical equipment and time requirement. It is possible to detect Chloramphenicol in intestine, honey, milk, whole egg, urine, serum, muscle and liver.
All reagents required for the enzyme immunoassay-including stands-are contained in the test kits.
The test kit is sufficient for 96 determinations(including stands)

Specification:96 wells/kit
Sensitivity: 0.05 ppb
Test linear dimensional: 0.05--4.05ppb

Detection limit:
egg, intestine …………………………0.1ppb
honey …………………………………0.15ppb
milk ……………………………………0.05ppb
urine and serum………………………0.1ppb
muscle and liver …………………… 0.05ppb

Sample preparation time:
egg, milk …………………………… 30min
serum ……………………………… 15min
urine ………………………………… 2.5h
muscle and liver, intestine ……… 20min

Recovery rate：
muscle and liver…………………………70-90%
honey ……………………………………60-80%
milk……………………………………… 70-100%

Cross-reactions:
Chloramphenicol…………………………100%
Chloramphenicol Palmitate……………… 5%
Thiamphenicol……………………………<0.1%
Florfeniol …………………………… <0.1%

Test implemention time: 2 h

Nitrofurantoin is synthetic broad-spectrum antibiotic and can kill various gram-positive and gram-negative bacteria, which is frequently employed in animal and aquaculture production. But Nitrofurantoin and its metabolite in animal food is passed to human through the food chain, regularly taking them can cause plenty of deseases, which can lead to cancer, teratogenesis and other side effects. Nitrofurantoin was banned from use in food animal production in the EU, and analysis of Nitrofurantoin was strictly implemented, along with this, America formulated the corresponding policy and regulations in 2002. The use of Nitrofurantoin was is classified as forbidden drugs in Veterinary Medicine and other Compound Detailed Banned in Food Animal Lists which was pulished by Ministry of Chinese Agriculture in March, 2002.
Our company develop AHD ELISA test kit, which is up to international advanced standard. It has advantages regarding sensitivity, specificity, accurate analysis of quota, samples pre-processing and time requirement. It is possible to detect nitrofurantoin metabolite residues in animal tissue (muscle, liver), honey, milk as well as the aquatic product (fish, shrimp) , etc.

All reagents required for the enzyme immunoassay-including stands-are contained in the test kits.
The test kit is sufficient for 96 determinations(including stands)

Specification : 96 wells/kit
Sensitivity: 0.1 ppb
Test linear dimensional: 0.1--8.1ppb

Detection limit:
honey and issue ……………………… 0.2ppb
fish and shrimp ……………………… 0.3ppb Sample preparation time:
fish and shrimp ……………………………2h
honey,meat and liver ………………………2h

Recovery rate：
fish and shrimp ……………………………75-105%
honey,meat and liver………………………65-95%

Cross-reations:
AHD ……………………………………… 100%
AOZ ……………………………………… ﹤0.1%
AMOZ ………………………………………﹤0.1%
SEM ……………………………………… ﹤0.1%

Test implemention time: 80 min

SEM ELISA Test Kit - Semicarbazide

Price: € 576 (art.code: 220-SEM-E)

Furacili is synthetic broad-spectrum antibiotic and can treat animal desease widely , which is frequently employed in animal and aquaculture production. But Furacilin and its metabolite residues in animal food are passed to human through the food chain , taking them regularly cause various diseases, which can lead to cancer, teratogenesis and other side effects. Furacilin was banned from use in food industry in Australia, Canada, Japan, Singapore, and analsysis of Nitrofuran was strictly implemented in Aquatic Products. Japanese positive list regulated that Nitrofuran medicine mustn’t be detected out. The use of Nitrofurantoin was are classified as forbidden drugs in Veterinary medicine And Other Compound Detailed Banned in Food Animal Lists which was pulished by Ministry of Chinese Agriculture in March 2002.
AHD-residues are determined most commonly by HPLC, LC-MS before. Enzyme immunoassays, compared with chromatographic methods, show quite advantages regarding sensitivty , simple process , technical equipment and time requirement.

Our company researchers make every efforts to solve some technical problems, firstly manufacture SEM ELISA test kit in the world .This test kit is used to detect SEM-residues in food based on enzyme inmmunoassays ,indirectly to detect Furacili. Compared with HPLC and LC-MS method, ELISA test result is coincident with chromatographic methods, which has considerable advantages regarding sensitivity, specificity, accurate analysis of quota , sample pre-processing and time requirement. It is possible to detect nitrofurantoin metabolite residue in animal tissue (muscle, liver), honey, milk as well as aquatic product (fish, shrimp), and is suitable to screen massive sample.

Specification : 96 wells/kit
Sensitivity: 0.1 ppb
Test linear dimensional: 0.1--8.1ppb

Detection limit:
honey, meat and liver……………………… 0.2ppb

Sample preparation time:
fish and shrimp ……………………………2h
honey,meat and liver ………………………2h

Recovery rate：
fish and shrimp ……………………………75-95%
honey,meat and liver………………………60-90%

Cross-reations:
SEM ……………………………………… 100%
AOZ ……………………………………… ﹤0.1%
AMOZ ………………………………………﹤0.1%
AHD ……………………………………… ﹤0.1%

Test implemention time: 80 min

Streptomycin ELISA Test Kit

Price: € 277 (art.code: 220-STM-E)

Streptomycin has visible side effect on nerve of audibility ,which is easy to lead to deafness and dangerous for allergic unborn child. In order to protect customers’ health, the EU and American regulated that Streptomycin in milk don’t have to supass 200ppb, in honey must be below 20ppb, in muscle and liver don’t have to supass 500ppb.

Streptomycin is most commonly determined by gas chromatography method, this method is cost and needs long time.The ELISA test kit shows considerable advantages regarding sensitivity, accuracy ,convinence ,technical equipment and time requirement. It is possible to detect Streptomycin residues in honey ,milk based on enzyme immunoassay .

Specification: 96 wells/kit

Gentamicin is aminoglycoside antibiotic ,which is employed in animal disease treatment.It has nerve toxicity and health effects for its residues in animal food.Gentamicin was banned from use in food animal production in the

EU ,America,China. At present , ELISA test method is considered to be analysis of Gentamicin and is widely used.

Competitive enzyme immunoassay, compared with instrumental analysis, indicates great advantages regarding sensitivity,detection limit,technical equipment and time requirement. It is possible to used in the quantitative analysis of Gentamycin in muscle and liver.
All reagents required for the enzyme immunoassay-including stands-are contained in the test kits.
The test kit is sufficient for 96 determinations(including stands)

Specification: 96 wells/kit
Sensitivity: 0.1 ppb
Test linear dimensional: 0.1—8.1ppb

Detection limit:
Chicken muscle and liver………………………… 1ppb

Recovery rate：
Chicken muscle and liver ……………………… 60-80%

Cross-reactions:
Gentamycin ……………………………………… 100%
Streptomycin …………………………………… 〈1%
Dihydrostreptomycin ……………………………〈1%
Neomycin ………………………………………… 〈1%

Test implemention time: 90 min

Tylosin is macrolide antibiotic, which can promote the animal growth, improve feed efficiency, control chicken chronic respiratory disease, treat dysentery and bacterial pneumonia, is used as special antibacterial agent and feed additive in poultry and livestock production.Its high distribution in body is easy to accumulate and cause chronic poisoning, liver damaged, auditory handicap. American Food and Drug Administration stipulated that permissible concentration of Tylosin in edible tissue and protein are 0.2ppm, in milk is 0.05ppm, in the chinese Agriculture Ministry 235th document stipulated that its maximum residues limit is 100 mu g/kg.
Competitive enzyme immunoassay for the quantitative analysis of Tylosin in honey, muscle and liver.
All reagents required for the enzyme immunoassay-including stands-are contained in the test kits.
The test kit is sufficient for 96 determinations(including stands)

Specification: 96 wells/kit
Sensitivity: 1.5 ppb
Test linear dimensional: 1.5—121.5ppb
Detection limit:
tissue (muscle, liver)……………………3ppb
honey ………………………………… 1.5ppb
Recovery rate：
chicken muscle and liver ……… 60-80%
Cross-reactions:
muscle…………………………… 65-95%
liver …………………………… 65-85%
honey ………………………………65-95%

Test implemention time: 90 min