Home Up Feedback Contents Search Sell your Prod. Meet us News           

Home • Up

Article 3




+32 1658 9045


0032 (0)16 41 44 07

+32 1650 9045


Av. de l' Armée 68

B-1040 Brussels



tel 01 43 25 01 50

fax01 43 25 01 60

9, rue Lagrange

75005 Paris


tel 02 36 00 65 93

fax 02 36 00 65 94

20135 Milano


tel +32 1658 9045

fax +32 1650 9045


Tel 058 710 33 44

Fax 00 32 16 50 90 45

ul. Grunwaldzka 88A/2

81-771 Sopot


tel +81 78 386 0860

fax +81 78 306 0296


Chuo-ku, Kobe



Canada Montreal

Česká republika Praha


Finland Helsset

Ελλάς Αθήνα

Magyarország Budapest

Ireland Dublin



Norge Oslo

Polska Warszawa

Sverige Stockholm

Schweiz Züri

US New York

Other Countries
0032 (0)16 41 44 07






How SAINT-PhD outperforms the competition  
SAINT-PhD efficiently delivers proteins under physiologically relevant conditions.

SAINT-PhD protein delivery reagent has been tested together with 5 competing protein
delivery reagents. Cells were plated at a density of approximately 100 thousand cells/well
in a 24-well plate one day before protein transfection. Cells were confluent the next day.
FITC labeled F(ab’)2 Rabbit-anti-Mouse IgG was used as protein of choice, because the
delivery of this labeled protein can easily be monitored by FACS analysis. Each reagent was
used according to its manufacturers protocol. Cells were incubated with the protein complexes
for 4 hours (37°C,5% CO2) without changing the medium (containing 10% FBS). FACS analysis
was performed after harvesting the cells.


Figure 1: Intracellular delivery of FITC-labeled IgG with 6 different protein delivery
reagents. Each reagent was used according to the manufacturers protocols. Cells were
incubated with the protein complexes for 4 hours (37°C,5% CO2) without changing the medium.
FACS analysis was performed after harvesting the cells.


From figure 1 it is clear that SAINT-PhD is the only reagent that performs well when
delivering relatively small amounts of proteins into cells growing in serum containing medium.
It is our opinion that these two advantages are very significant for our customers.

The ability to deliver small quantities of proteins saves a lot of money, because most
proteins of interest are either very expensive or simply impossible to obtain in large quantities.
Furthermore, introducing large quantities of a certain protein in a cell will certainly disturb
the cellular machinery and will likely not resemble any type of naturally occurring event.
When studying naturally occurring processes, it is important to be able to deliver small amounts
of the protein to be studied.

The possibility to deliver proteins to cells in the presence of serum keeps the cells healthier
and more viable. And although the SAINT-PhD reagent is primarily meant for in vitro application,
the performance in the presence of serum suggests that also in vivo protein delivery might be
a possibility.

From this comparison of protein delivery reagents we conclude that our SAINT-PhD reagent is the
reagent best suited for intracellular protein delivery under physiologically relevant conditions.


Copyright © 2002 GENTAUR Molecular Products
Last modified: 05/29/09