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+32 1658 9045

or

0032 (0)16 41 44 07

+32 1650 9045

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Av. de l' Armée 68

B-1040 Brussels

BELGIUM

France

tel 01 43 25 01 50

fax01 43 25 01 60

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75005 Paris

Italia

tel 02 36 00 65 93

fax 02 36 00 65 94

20135 Milano

Deutschland

tel +32 1658 9045

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Polska

Tel 058 710 33 44

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ul. Grunwaldzka 88A/2

81-771 Sopot

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Other Countries
0032 (0)16 41 44 07


 

 

 

 

 

 
Fluorescent & Luminescent Reagents
 
 
Fluorescent Calcium Indicators and Related Products
Fluorescent Cl, pH and Zinc Indicators
Fluorescent Membrane and Membrane Potential Dyes
Natural Products and Their Fluorescent Conjugates
Biochemical Reagents for Nitric Oxide (NO) Studies
Enzyme Substrates
Reactive Dyes and Related Reagents
Biotin Derivatives
Nucleic Acid Stains and Products for Genomics Studies
Cytosolic Tracers and Related Products
Protein Conjugates
Proteomics products
   
Bioscience Assay Kits
Apoptosis Assays
Viability/Cytotoxicity Assays
Reporter Gene Assays
Cell Biology Assays
qPCR

 

Ultra Sensitive, Super Speedy and Much more...
LumiteinTM Protein Gel Stain

Pricelist

HIGHLIGHTS
- Highly Sensitive
At least as sensitive as silver stain by detecting as little
as 1 ng or less protein.
- No Fixation Required
- Extremely Simple & Fast Staining
Use the 30-min Rapid Protocol for excellent result, or the
90-min Basic Protocol for the ultimate sensitivity; no
overstaining with longer staining time.
- Super Fast Destaining
Destain in as little as 10 min using MeOH/H2O, or in 20 min
using water only.
- Very Low Background
- Excellent Compatibility with Existing Instruments
Can be used with either a simple UV-box (designed for
DNA gel viewing), a Dark Reader, or a high-end laser
scanner (See Figure 1 for spectra).
- Wide Linear Detection Range
At least three orders of magnitude.
- Perfectly Compatible with Downstream Analysis
Compatible with MS and sequencing.
- Stable
Staining solution stable at room temperature for at least 1
year.


Glowing Products for ScienceTM
Figure 1. Two-fold serial dilutions of Precision Plus protein standard (Bio-Rad) were
separated via SDS-PAGE and then stained with either LumiteinTM or SYPRO Ruby according to
the manufacturer-recommended protocols. Images were taken with GE Typhoon Trio using 532
nm excitation and 610BP30 emission filter.
Lumitein-
SYPRO- Ruby

* Lumitein and its related technologies are covered by pending US and international patents.
** SYPRO is a registered trademark of Molecular Probes, Inc.
page

 
Coomassie Blue, SYPRO Ruby (Invitrogen), Deep-purple (GE) and Krypton (Piece).

Figure 1. Two-fold serial dilutions of Precision Plus protein standard (Bio-Rad) were
separated via SDS-PAGE and then stained with either LumiteinTM or SYPRO Ruby according to
the manufacturer-recommended protocols. Images were taken with GE Typhoon Trio using 532
nm excitation and 610BP30 emission filter


Lumitein

- Protein Gel Stain is a luminescent dye designed for
detecting proteins in polyacrylamide gels in connection with SDS-PAGE.
The dye has many of the same desirable features that SYPRO Ruby
possesses, such as detection of as little as 1 ng or less of proteins,
compatibility with both UV and visible light excitation, excellent
photostability and a detection linearity range of at least 3 orders of
magnitude. However, Lumitein- has some important advantages over
SYPRO Ruby and other commercial protein gel stains. Protein staining
using Lumitein- is far simpler and faster than that using SYPRO Ruby.
With Lumitein-, protein fixation and staining is a single combined 90-
minute incubation process. In addition, gel staining using Lumitein-
generally has lower background than that using SYPRO Ruby. Finally,
Lumitein- is supplied as a 5X concentrated solution, which saves
shipping cost and provides convenience for storage and handling.
Lumitein- protein gel staining is compatible with downstream protein
analysis such as mass spectrometry and Edman-based sequencing.
Table 2. Lumitein- gel stain price list
Table 1. Comparison of staining protocols
Protocol Step SYPRO- Ruby LumiteinTM
 

Fixation
step 1
15 min 50%
methanol/7.5% acetic
acid
none
Fixation
step 2
15 min 50%
methanol/7.5% acetic
acid
none
Staining overnight
90 min (Basic Protocol)
or
30 min (Rapid Protocol)
Destaining 30 min 10%
methanol/7% acetic acid
5 min 30%methanol/15%
acetic acid (or no
destaining)
Rinse step 1 5 min water single 5 min water
rinse (or 20 min water
rinse without destaining)
Rinse step 2 5 min water
Figure 4. 2-D gel of human liver protein lysate stained with Lumitein-. The
three circled spots were picked for MS analysis by Applied Biomics, Inc.
(Hayward, CA). The result confirmed that Lumitein- gel staining is fully
compatible with MS analysis (data not shown).


Figure 4. 2-D gel of human liver protein lysate stained with Lumitein-. The
three circled spots were picked for MS analysis by Applied Biomics, Inc.
(Hayward, CA). The result confirmed that Lumitein- gel staining is fully
compatible with MS analysis (data not shown).

 

Pricing

Lumitein  125 Euro/40mL

 255 Euro/200mL

752 Euro /1L

Lumitein is sold in 5X concentration.

 

Pricelist

 


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Last modified: 05/29/09