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H-FABP ELISA

■Application

Quantification of H-FABP in human serum and plasma

■Assay procedure for MARKIT-M H-FABP

■Contents of MARKIT-M H-FABP

・Each kit (96 tests) contains the following reagents.

・Standard 0 (lyophilized): 1 vial (for2.0 mL).

・Standard 5 (lyophilized): 1 vial (for 0.5 mL) contains 2.5 ng of H-FABP*.

・Standard 10 (lyophilized): 1 vial (for 0.5 mL) contains 5 ng of H-FABP*.

・Standard 25 (lyophilized): 1 vial (for 0.5 mL) contains 12.5 ng of H-FABP*.

・Standard 50 (lyophilized): 1 vial (for 0.5 mL) contains 25 ng of H-FABP*.

・Standard 100 (lyophilized): 1 vial (for 0.5 mL) contains 50 ng of H-FABP*.

・Standard 250 (lyophilized): 1 vial (for 0.5 mL) contains 125 ng of H-FABP*.

・H-FABP antibody-coated wells: 1 plate (96 wells).  Each well contains: anti-human H-FABP monoclonal antibody.

・Buffer solution (bottle No. 1): 1 bottle (10 mL).

・Wash buffer concentrate (bottle No. 2): 3 bottles (30 mL each).

・H-FABP antibody-enzyme conjugate (bottle No. 3): 1 bottle (15 mL), containing HRP-labeled anti-human H-FABP monoclonal antibody.

・Substrate tablets: 3.  Each tablet contains o-phenylenediamine dihydrochloride (OPD) (13 mg).

・Substrate diluent buffer (bottle No. 4): 3 bottles (15 mL each). Each bottle contains hydrogen peroxide (15μL).

・Stop solution (bottle No. 5): 1 bottle (15 mL).

・Microplate for dilution: 1 plate (96 wells).

・Graph paper: 3 sheets.

*H-FABP: Human H-FABP is quantitatively determined by an immunological method using purified human H-FABP as a standard material.

■Performance

1. Reproducibility

When two distinct samples (H-FABP, 50-200 ng/mL) are determined 10 times simultaneously, the coefficient of variation in their absorbances should be less than 10 %.

2. Assay range

H-FABP 1.25 -250 ng/mL

■Cut-off level of H-FABP

A mass concentration of 6.2 ng of H-FABP per mL of serum or plasma is designated as the cut-off value for the diagnosis of acute myocardial infarction.  For clinical assessment this value shows the highest diagnostic efficacy.  An H-FABP mass concentration of 6.2 ng/mL of serum or plasma or higher is strongly suggestive of cardiac damage.

■Storage method and expiry period

Storage: Store in a cool place (2-10^oC), protected from light.  Avoid freezing.

Expiry period: 2 years

■References

1) Ockner, R.K., et al.: Mol. Cell. Biochem. 98: 3-9, 1990.

2) Kaikaus, R.M., et al.: Experientia 46: 617-630, 1990.

3) Ohkaru, Y., et al.: J. Immunol. Methods 178: 99-111, 1995.

4) Tsuji, R., et al.: Int. J. Cardiol. 41: 209-217, 1993.

5) Sohmiya, K., et al.: J. Mol. Cell. Cardiol. 25: 1413-1426, 1993.

6) Tanaka, T., et al.: Clin. Biochem. 24: 195-201, 1991.

7) Paulussen, R.J.A., et al.: Int. J. Biochem. 22: 393-398, 1990.

8) Adams, J.E. III, et al.: Circulation 88: 750-763, 1993.

9) Pauleo, P.R. and Roberts, R.: Cardiol. Clin. 6: 97-109, 1988.

10) Veerkamp, J.H., et al.: Prog. Lipid Res. 34: 17-52, 1995.

11) Glatz, J.F.C., et al.: Br. Heart J. 71: 135-140, 1994.

12) Wodzig, K.W.H., et al.: Eur. J. Clin. Chem. Clin. Biochem. 35: 191-198, 1997.

Copyright © 2002 GENTAUR Molecular Products
Last modified: 05/29/09