H-FABP ELISA
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■Application Quantification of H-FABP in human serum and plasma ■Assay procedure for MARKIT-M H-FABP
・Each kit (96 tests) contains the following reagents. ・Standard 0 (lyophilized): 1 vial (for2.0 mL). ・Standard 5 (lyophilized): 1 vial (for 0.5 mL) contains 2.5 ng of H-FABP*. ・Standard 10 (lyophilized): 1 vial (for 0.5 mL) contains 5 ng of H-FABP*. ・Standard 25 (lyophilized): 1 vial (for 0.5 mL) contains 12.5 ng of H-FABP*. ・Standard 50 (lyophilized): 1 vial (for 0.5 mL) contains 25 ng of H-FABP*. ・Standard 100 (lyophilized): 1 vial (for 0.5 mL) contains 50 ng of H-FABP*. ・Standard 250 (lyophilized): 1 vial (for 0.5 mL) contains 125 ng of H-FABP*. ・H-FABP antibody-coated wells: 1 plate (96 wells). Each well contains: anti-human H-FABP monoclonal antibody. ・Buffer solution (bottle No. 1): 1 bottle (10 mL). ・Wash buffer concentrate (bottle No. 2): 3 bottles (30 mL each). ・H-FABP antibody-enzyme conjugate (bottle No. 3): 1 bottle (15 mL), containing HRP-labeled anti-human H-FABP monoclonal antibody. ・Substrate tablets: 3. Each tablet contains o-phenylenediamine dihydrochloride (OPD) (13 mg). ・Substrate diluent buffer (bottle No. 4): 3 bottles (15 mL each). Each bottle contains hydrogen peroxide (15μL). ・Stop solution (bottle No. 5): 1 bottle (15 mL). ・Microplate for dilution: 1 plate (96 wells). ・Graph paper: 3 sheets. *H-FABP: Human H-FABP is quantitatively determined by an immunological method using purified human H-FABP as a standard material. ■Performance 1. Reproducibility When two distinct samples (H-FABP, 50-200 ng/mL) are determined 10 times simultaneously, the coefficient of variation in their absorbances should be less than 10 %. 2. Assay range H-FABP 1.25 -250 ng/mL
■Cut-off level of H-FABP A mass concentration of 6.2 ng of H-FABP per mL of serum or plasma is designated as the cut-off value for the diagnosis of acute myocardial infarction. For clinical assessment this value shows the highest diagnostic efficacy. An H-FABP mass concentration of 6.2 ng/mL of serum or plasma or higher is strongly suggestive of cardiac damage. ■Storage method and expiry period Storage: Store in a cool place (2-10oC), protected from light. Avoid freezing. Expiry period: 2 years ■References 1) Ockner, R.K., et al.: Mol. Cell. Biochem. 98: 3-9, 1990. 2) Kaikaus, R.M., et al.: Experientia 46: 617-630, 1990. 3) Ohkaru, Y., et al.: J. Immunol. Methods 178: 99-111, 1995. 4) Tsuji, R., et al.: Int. J. Cardiol. 41: 209-217, 1993. 5) Sohmiya, K., et al.: J. Mol. Cell. Cardiol. 25: 1413-1426, 1993. 6) Tanaka, T., et al.: Clin. Biochem. 24: 195-201, 1991. 7) Paulussen, R.J.A., et al.: Int. J. Biochem. 22: 393-398, 1990. 8) Adams, J.E. III, et al.: Circulation 88: 750-763, 1993. 9) Pauleo, P.R. and Roberts, R.: Cardiol. Clin. 6: 97-109, 1988. 10) Veerkamp, J.H., et al.: Prog. Lipid Res. 34: 17-52, 1995. 11) Glatz, J.F.C., et al.: Br. Heart J. 71: 135-140, 1994. 12) Wodzig, K.W.H., et al.: Eur. J. Clin. Chem. Clin. Biochem. 35: 191-198, 1997. |
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