Human Integrin
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Human INTEGRIN αVβ3 (Octyl- β-D-glucopyranoside Formulation) PRODUCT NO 01-INT-4 634 EUR PACKAGE SIZE 50 μg DESCRIPTION Purified protein (0.435 mg/mL) in 25 mM Tris-HCI, pH 7.4, 150 mM NaCI, 1 mM MgCI 2,0.1 mM CaCI 2 and 10 mM Octyl-β-D-glucopyranoside. No preservatives.SOURCE Human placenta. PURIFICATION Integrin αVβ3 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to αVβ3 integrin. A tissue detergent extract, which was applied to the column. Purity is >95 % by SDS-PAGE (silver stain). It has tested negative for HIV & Hepatitis. It is expected that this preparation of αVβ3 integrin will also interact with other αVβ3 binding proteins in an RGD-dependent fashion. STORAGE Maintain at -70 °C in undiluted aliquots. Avoid repeated freeze/ thaw cycles. Handle as potentially infectious. APPLICATION Electrophoresis and Immunoblotting control. Also useful for in vitro binding experiments with metalloproteinase MMP-2. This format of αVβ3 is appropriate for functional evaluations. REFERENCE Belkin, V.M., et al. J. Cell Biol. (1990) 111: 2159-2170 Belkin, V.M., et al. Biochem. and Mol. Biol. Inter. (1996) 40: 53-60. Petela, R., et al. Methods Enzymol. (1987) 144: 475- 489. |
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