Modulus™ Single Tube Multimode Reader
A modular, easy to use, and cost-effective single tube multimode
reader for Luminescence, Fluorescence, and Absorbance Life Science
applications.
Overview
The Modulus™ Single Tube Multimode Reader is
designed to provide the utmost flexibility. In addition to high
performance, the Modulus™ blends user-friendly operation and a small
footprint with flexible purchasing options. The result of this design is
an instrument with superior performance that is easy to use, affordable,
and can be customized to your laboratory’s needs.
Performance
The Modulus™ combines the superior performance
expected from single-mode instruments with the functionality of multiple
modes. To achieve high performance, the Modulus™ is designed with
optical channels dedicated to each individual technology. Unlike other
multimode systems, readings taken with the Modulus™ are not degraded by
indirect fiber-optic transmission or crowded optical channels. Dedicated
optical channeling ensures that the Modulus™ provides sensitivity and
dynamic range on par with single-mode instruments.
Ease of Use
The Modulus™ is designed to be put into use
straight from the box without the need to read a manual or obtain
special training. To achieve this plug-and-play usability, the Modulus™
combines a color touch screen with an intuitive user interface. The
interface makes running samples and viewing data fast and simple while
also maintaining the flexibility needed for advanced or custom
protocols.
Affordable Modularity
The Modulus™ is a modular instrument that fits
easily into most budgets. Purchase the technology or modes that you need
now and add onto the system later as your needs expand. For example, the
Modulus™ can be purchased as a Luminometer, then Fluorescence and/or
Absorbance Modules can be purchased and added later. There’s no service
call or downtime. With the modular design, changing technologies is as
easy as snapping in a module and restarting the instrument.
Minicell Adapter
For fluorescence measurements of sample volumes between 100 µl and 200
µl, a minicell adapter is available as an optional accessory.
Lid Start
Adjust settings to start measurement immediately after the lid is
closed.
Sample Formats
For luminescence measurements, the Modulus™ accepts 1.5-ml
microcentrifuge tubes. For fluorescence and absorbance measurements, the
Modulus accepts standard 10 x 10 mm cuvettes.
Touchscreen
The touchscreen allows simple navigation between screens for setting
parameters, setting calibrations, and starting runs.
Troubleshooting
Easy access to HELP with the touch of a button.
Data Handling
The Modulus™ stores up to 18 calibrations. Data from the last 20
measurements are displayed on the color LCD screen. Optional accessories
for easy data exportation include Spreadsheet Interface Software or
thermal printer.
Luminescence Applications
• Luciferase Assays
• ATP Assays
• Chemiluminescent Immunoassays
• Kinetics Assays
The Modulus™ with a Luminescence Module is designed to deliver
performance equivalent to dedicated single-tube luminometers while also
offering the flexibility of a multimode reader. To achieve sensitivity
on par with that of a dedicated luminometer, the luminescence channel is
separated from other measurement technologies and positioned directly
below the sample well. These conditions maximize light capture for the
best possible sensitivity. In conjunction, a low-noise photomultiplier
tube ensures that collected light is not compromised. The Modulus™ also
has greater than 5 logs of dynamic range. This dynamic range is more
than adequate to cover common luminescence applications, thus reducing
the need to dilute samples.
Fluorescence Applications
• DNA/RNA Quantitation
• Gene Expression Assays
• Enzyme Assays
• Protein Quantitation
The Modulus™ with a Fluorescence Module is designed to deliver both
high performance and user flexibility. To achieve high performance, each
Fluorescence Module utilizes powerful light-emitting diodes (LEDs) as
excitation sources. LEDs have very specific light-output profiles which
closely match the excitation profile of commonly used fluorescent
molecules. LED usage increases sensitivity by fully exciting the
fluorophore and reducing non-specific light leakage, a problem often
found when using broad-spectrum light sources. Additionally, the
highly-sensitive Modulus™ uses a 90-degree fluorescent design to
minimize light scatter and lower background resulting in high
sensitivity.
The Modulus™ as a fluorometer utilizes four easily exchangeable
modules to measure the most popular fluorophores. In addition, custom
modules can be made for non-standard applications.
Fluorescence Module
|
Typical Fluorophores
|
UV
(Ex 365 nm, Em 410 - 450 nm) |
Hoechst Dye, 4-methyl-umbelliferone (4-MU) |
Blue
(Ex 460 nm, Em 515 - 570 nm) |
EGFP, rAcGFP, PicoGreen®,
RiboGreen®, Fluorescein, Quant-iT™ Protein |
Green
(Ex 525 nm, Em 580 - 640 nm) |
Rhodamine, Cy3, RFP |
Red
(Ex 625 nm, Em 660 - 720 nm) |
Cy5, Quant-iT™ RNA |
Absorbance Applications
• Protein Quantitation
• Cell Turbidity, OD 600
The Modulus™ with the Absorbance Module provides measurements that
are highly sensitive and cover a wide dynamic range. Because the optics
are not shared with any other detection technology, the focused
absorbance measurements yield outstanding performance. The absorbance
channel has a large reading range of 0 - 4 OD with an accuracy that
deviates less than 0.7%. The Modulus™ comes with three filter paddles
with factory-installed filters for measuring 560, 600, and 750 nm. These
filters accommodate the most common protein assays. Filter paddles can
be easily exchanged in seconds. In addition, custom filter paddles can
be readily made for non-standard applications.
Modulus Single Tube
Instrument Specifications
Detection Modes: Luminescence, Fluorescence, Absorbance
Read Type: Glow, Kinetic, Repeat
Sample Format: 10 x 10 mm cuvette, 100 - 200 µl minicell vial
(optional), 1.5 ml microfuge tube (Luminescence)
User Interface: Touch screen navigation and operation
Data Output: Data displayed on screen or connect to PC (not included)
via serial cable to download. Optional thermal printer available.
External PC Requirements (optional): Windows 98 or later
Computer Interface: 100% ASCII format through a 9-pin RS-232 serial
cable at 9600 baud rate
Power: 12 V 0.84 A Max
Auto Shutoff: Touch screen hibernates after 15 min of inactivity
Dimensions: 12.92” D x 10.44” W x 8.42” H (32.82 cm D x 26.52 cm W x
21.39 cm H)
Weight: ~6.5 lbs (2.95 kg)
Operating Temperature: 60 - 105 şF (15 - 40 şC)
Warranty: One year
Approvals: CE
Luminescence Module Specifications (Factory Installed)
Detector: Photomultiplier tube (PMT)
Spectral Range: 350 - 650 nmPeak Wavelength: 420 nm
Detection Limit: 1 x 10-16 moles ATP
Linear Dynamic Range: 5 logs
Sample Format: 1.5 ml microfuge tubes
Fluorescence Module Specifications (User Installable)
Light Source: Wavelength-matched LED
Detector: Photodiode
Wavelength Selection: Snap-in Fluorescence ModulesFluorescence
Modules:P/N 9200-040 Blue (Ex 460 nm, Em 515 - 570 nm)P/N 9200-041 UV
(Ex 365 nm, Em 410 - 450 nm)P/N 9200-042 Green (Ex 525 nm, Em 580 - 640
nm) P/N 9200-043 Red (Ex 625 nm, Em 660 - 720nm)
P/N 9200-044 UV-GFP (Ex 365 nm, Em 515 - 570nm)
Detection Limit: 6 ppt fluorescein, 4.5 pg/100 µl DNA with PicoGreen®
Dye
Linear Dynamic Range: 6 logs, assay dependent
Sample Format: 10 x 10 mm cuvette, 100 - 200 µl minicell vial (optional)
Read Out: Direct concentration or raw fluorescence
Calibration: 1 - 5 point calibration
Discrete Sample Average: Sample readings are averaged over 5 seconds to
improve accuracy
Absorbance Module Specifications (9200-050 User Installable)
Light Source: LED
Detector: Photodiode
Spectral Range: 400 - 800 nm
Wavelengths for Installed Filters: 560, 600, 750 nm
Photometric Measuring Range: 0 - 4.0 OD
Linear Dynamic Range: 0 - 2.5 OD, assay dependent
OD Accuracy: ±0.7%
OD Precision: <±0.5% at 1.0 OD
For research use only. Not for use in diagnostic procedures. Modulus
is a trademark of Turner BioSystems, Inc. All other trademarks are the
sole property of their respective owners. |