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GENTAUR
 +32 1658 9045
or
0032 (0)16 41 44 07
 +32 1650 9045
[email protected]
Av. de l' Armée 68
B-1040 Brussels
BELGIUM
France
tel 01 43 25 01 50
fax01 43 25 01 60
9, rue Lagrange
75005 Paris
Italia
tel 02 36 00 65 93
fax 02 36 00 65 94
20135 Milano
Deutschland
tel +32 1658 9045
fax +32 1650 9045
Polska
Tel 058 710 33 44
Fax 00 32 16 50 90 45
ul. Grunwaldzka 88A/2
81-771 Sopot
日本
tel +81 78 386 0860
fax +81 78 306 0296
Minaatojimaminami-manchi
Chuo-ku, Kobe
065-0047
Österreich
+43720880899
Canada Montreal
+15149077481
Česká republika Praha
+420246019719
Danmark
+4569918806
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+358942419041
Ελλάς Αθήνα
+302111768494
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+3619980547
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+35316526556
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+35220880274
Nederland
+31208080893
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+4721031366
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+48223988221
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+46852503438
Schweiz Züri
+41435006251
US New York
+17185132983
Other Countries
0032 (0)16 41 44 07
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| The e-MycoTM
(ver.2.0) product is a set of primers
designed to detect the presence of
mycoplasma that might contaminate
biological materials such as cultured
cells. Conventional techniques that are
used to detect mycoplasma involve
culturing samples on selective media,
which needs at least 1 week to obtain
results, whereas by performing PCR with
this primer set, which is based on
conserved 16S rRNA, detection results
are obtained in a few hours. Because the
presence of contaminant mycoplasma can
be easily detected by only verifying the
bands of amplified DNA fragments using
electrophoresis, there is no need to
prepare probes that are labeled with
radioisotope, etc. The adopted
8-methoxypsoralen (8-MOP) is used to
extinguish the template activity of
contaminated DNAs.
An internal control of this product
was constructed to identify false
negative results in each reaction. The
internal control was designed in such a
way that the sample primer pair was used
to amplify the internal control and
target DNA, which were differentiated by
size. Each tube of the e-Myco¢â
Mycoplasma PCR Detection Kit (ver.2.0)
contains all the components for PCR
except for template: i-StarTaqTM
DNA Polymerase, dNTPs, 10x Buffer,
primers, 8-MOP, and internal control for
Mycoplasma partial gene amplifications.
So, you can just add your templates and
perform the PCR reaction. |
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• Premix Type
- This e-Myco¢â Mycoplasma PCR Detection
Kit(ver.2.0) contains all the components for the PCR
reaction. You just add template DNA or samples.
• Broad Species Detection
- You can detect five common cell culture-infecting
species of mycoplasma and also other various
species of mycoplasma (See Technical Guide).
• Species Determination
- You can determine the species of mycoplasma by
sequencing the amplified PCR products.
• Internal Control
- Internal control embedded in the product prevents
misjudgment that possibly arises from an erroneous
PCR test.
• Elimination of Carryover Contamination
System
- 8-MOP solution prevents carryover contamination
by PCR products.
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