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Hog cholera virus is high pathogenic and lethal rate, which is able to bring massive loss to aquaculture.It is infectious to touch high pathogenic virus for swine in incubation period, pig excretes much toxicity before appearance of accute or subcute clinical symptom of cholera virus;then, swine stops excreting toxicity, obvious CSFV antibody is present in swine body. Swine which infects equable virus appears infection, long-term or intermittent excreting toxicity before dying of illness. Pregnant swine infects embryo through the placenta to cause miscarriage, weakness, abortion, etc. Swine infected weak virus congenitally come out persistent infection of little swine, it shows immunological tolerance, excretes toxiciy and doesn’t generate any symptom. Swine may be infected by BVDV and BDV. It is importmant to distinguish hog cholera virus , BVDV and BDV effectively although this process is equable and self limited.
Hog cholera virus antibody ELISA test kit based on enzyme immunoassay to detect hog cholera virus antibody in the serum and plasma with HC-coated microwell.

Intended use
Hog cholera virus antibody ELISA test kit is widely used to detect HC-antibody in the serum, evaluate HC-vaccine immnunity in swine

field , investigate HC- epidemiology.

Discription and principles
The test kit is composed of HC-coated microwell, enzyme-conjugated anti-IgG, stop solution ,etc; enzyme immunoassay for the detection of antibody in swine serum to the HC-antigen. During the first incubation, diluted control-porcine serum and test serum, are added, if specific HC-antibodies is present in the test serum, HC-antibodies combine HC-antigens on the microwell, unconjugated antibodies and other ingredients is removed by washing; then putting enzyme-conjugated antibodies, is specific to react with antibody-antigens complex on the microwell, the unreacted conjugate is removed by washing, and a TMB substrate solution is added, in the presence of enzyme, the substrate is converted to a blue product, then the HF solution is added to stop reaction,The absorbance at 630nm, OD value is measured by spectrophotometer for very microwell.
 

Reagents provided

1. HCV-coated plates	2 pieces (96 wells)
2. Porcine Negetive Control-Porcine serum(green cap)	1 tube(1ml)
3. Porcine PositiveControl-Porcine serum (red cap)	1 tube(1ml)
4. Anti-pig IgG-HRP conjugate (yellow cap)	1 bottle(22m
5. Wash Concentrate(20xconcentrate) (white cap)	1 bottle(30ml)
6. Substrate A (red cap)	1 bottle(12ml)
7. Substrate B solution(black cap)	1 bottle(12ml)
8. Stop solution (blue cap)	1 bottle(12ml)
9. Sample Diluent	1 bottle (50ml)

  

Specification: 96wells x 2
Expire : 6months
Storage : 2-8 ℃, in dark place

                                                     
        
          

Swine foot and mouth is infectious disease which is an acute, thermal and high contact, caused by Picornaviridae, Rhinovirus of swine foot and mouth virus. It emerges different sizes blisters and ulcer in the hoof crown, heel, forks, vice- hoof ,skin, oral angle, cheek, tongue mucosa part, etc. Swine foot and mouth disease is of high incidence , fast infection, prevalent wide, which can easily lead to abortion, high dealth of little swine,and large economic loss.This disease is internationallly considered to be important prevention.

 

Intended use
Swine foot and mouth disease antibody test kit is frequently employed in the detection of O-FMD antibody in the serum, evaluation of vaccinated swine field , investigation of the epidemiology of swine foot and mouth disease .

Discription and principles
This test kit is made into antigen-coated microwell with purified, inactivated, concentrated antigen, enzyme immunoassay for the detection of antibody in the swine serum to the FMD-antigen. During the first incubation, diluted control-porcine serum and test serum are added, if specific FMD-O-antibodies is present in the test serum, FMD-antibodies react with FMD-O-antigens on the microwell, subsequent to a wash step; then adding enzyme-conjugated antibodies, is specific to react with antibody-antigens on the microwell, the unreacted conjugate is removed by washing, and a TMB substrate solution is added. In the presence of enzyme, the substrate is converted to a blue product, then the HF solution is added to stop reaction.The absorbance at 630nm, OD value is measured by spectrophotometer for very microwell.

 

Reagents provided

1. FMD-O-coated plates	2 pieces (96 wells)
2. Porcine Negetive Control-Porcine serum(green cap)	1 tube(1ml)
3. Porcine Positive Control-Porcine serum (red cap)	1 tube(1ml)
4. Anti-pig IgG-HRP conjugate (yellow cap)	1 bottle(22ml)
5. Wash Concentrate (20xconcentrate) (white cap)	1 bottle(30ml)
6. Substrate A solution (red cap)	1 bottle(12ml)
7. Substrate B solution (black cap)	1 bottle(12ml)
8. Stop solution (blue cap)	1 bottle(12ml)
9. Sample Diluent (transparent cap)	1 bottle (50ml)

 
Specification: 96wells x 2
Expire : 6 months
Storage : 2-8 ℃, in dark place

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Porcine parvovirus disease is caused by parvoviridae, which can lead to pregnant obstruction. It is high infectious, epidemic, outbreak. During 3 months, infectious rate of the group is up to 100%. Infected female swine primiparously comes into dead, abnormal and mummy fetus, no other symptoms appear out. This disease accurs in swine field, especial intensive field, which generate great hazard. Therefore, it is necessary to take measures to prevent it.

Intended use
Porcine parvovirus ELISA test kit is frequently employed in the detection of porcine parvovirus based on serologically diagnostic method, evaluation of PPV-vaccine immnunity in pig field and investigation of PPV- epidemiology.

Discription and principles
This test kit is made of antigen-coated microwell with purified, inactivated, concentrated antigen, enzyme immunoassay for the detection of antibodies in swine serum to the PPV-antigen. During the first incubation, add diluted control-porcine serum and test serum, if specific PPV-antibodies is present in the test serum, PPV-antibodies combine PPV-antigens on the microwell, subsequen to a wash step;then put in enzyme-conjugated antibodies, is specific to react antibody-antigens on the microwell, the unreacted conjugate is removed by washing, and a TMB substrate solution is added, in the presence of enzyme, generating a blue product, then the HF solution is added to stop reaction.The absorbance at 630nm, OD value is measured by spectrophotometer for very microwell.

Swine Pseudorabies Antibody ELISA Test Kit (PRV)  

Price: € 200        (art.code: 220-PRV-E)

 

Swine pseudorabies is acute and infectious disease, diffused among animals , caused by herpesviridae of alphaherpesvirinae

pseudorabies-I. When swine infects pseudorabies,. there are different symptoms for different ages, the old is not serious ,female shows reproductive obstruction, little pig is most serious, death rate of 15-days-old pig is 100%, great loss is involved in pig-breeding. Other animals present fever, tickling, and fatal infection of encephalomyelitis. Pseudorabies occurs widely in spring and and winter in the world.

 

Intended use
Swine pseudorabies ELISA test kit is frequently employed in the detection of pseudorabies antibodies by serologically diagnostic method, evaluation of pseudorabies-vaccine immnunity in pig field ,investigation of PRV- epidemiology.

Discription and principles
This test kit is made of antigen-coated microwell with purified, inactivated, concentrated antigen, enzyme immunoassay for the detection of antibody in swine serum to the PRV-antigen. During the first incubation, diluted control-porcine serum and test serum are added, if specific PRV-antibodies are present in the test serum, PRV-antibodies combine PRV-antigens on the microwell, subsequen to a wash step;then putting in enzyme-conjugated antibodies,is specific to react with antibody-antigens on the microwell, the unreacted conjugate is removed by washing, and a TMB substrate solution is added, in the presence of enzyme, the substrate generates a blue product, then the HF solution is added to stop reaction.The absorbance at 630nm, OD value is measured by spectrophotometer for very microwell.

 

Reagents provided

1. PRV-coated plates	2 pieces (96well)
2. Porcine Negetive Control-Porcine serum(green cap)	1 tube(1ml)
3. Porcine Positive Control-Porcine serum (red cap)	1 tube(1ml)
4. Anti-pig IgG-HRP conjugate (yellow cap)	1 bottle(22ml)
5. Wash Concentrate (20xconcentrate) (white cap)	1 bottle(30ml)
6. Substrate A solution (red cap)	1 bottle(12ml)
7. Substrate B solution (black cap)	1 bottle(12ml)
8. Stop solution (blue cap)	1 bottle(12ml)
9. Sample Diluent (transparent cap)	1 bottle (50ml)

Specification: 96wells x 2
Expire : 6 months
Storage : 2-8 ℃,in dark place
 

 

Porcine Parvovirus Indirect Haemagglutination Assay Test Kit

Price: € 20        (art.code:220-PIHA)

 

Principles
Positive indirect haemagglutination assay (IHA) is employed in the detection of antibodies in the unknown serum with the known blood antigens. Under given conditions, antigens reacting with the corresponding antibodies generate complex, which can’t be observed by eyes. If special processed red blood cells conjugates antigens, reactive delicacy of antigens and antibodies is improved quitely, under this conditions, red blood cells sensitized by porcine parvovirus react with porcine parvovirus antibody, agglutination of red blood cells is clearly observed.

 

Porcine Encephalitis Virus Indirect Haemagglutination Assay Test Kit

Price: € 20        (art.code:220-EIHA)

 

Principles
Positive indirect haemagglutination assay (IHA) is used to detect antibodies in unknown serum with the known blood antigens. Under given conditions, antigens reacting with the corresponding antibodies generates complex, which can’t be observed by eyes. If special processed red blood cells conjugates antigens, reactive delicacy of antigens and antibodies is improved quitely, under this conditions, red blood cells sensitized by porcine encephalitis virus reacts with porcine encephalitis virus antibody, agglutination of red blood cells is clearly observed.

Intended Use
The test kit is frequently used in serological survey in animals immunized with porcine encephalitis virus .
 

Equipment Required But Not Provided:
1. 96-well 110。Ⅴ medical haemagglutination plate, glass plate.
2. Min transferpette, Pipette tips
3. Mini vortexer

Reagents Provided
1. JEV-haemagglutination Antigens: reddish brown after shaking; if placed, anemia settled at the bottom of bottle
2. Porcine Negitive Control –porcine serum: light yellow and little clear viscous liquid
3. Porcine Positive Control –porcine serum: reddish or light yellow muddy viscous liquid
4. Diluent: light yellow or colorless liquid, store at low tempareture, form a tiny crystals at the bottom of bottle, dissolved at heated condition, no effect on use
5.Test serum, (0.5ml/unit) heat 30 min in 56℃ water

Packing
1. Liquid haemagglutination Antigens: shaking before use, 15ml /bottle, suitable for 40 tests
2. Porcine Negitive Control –porcine serum: direct dilution to use
3. Porcine Positive Control –porcine serum: direct dilution to use
4. Diluent: 15 ml/bottle, store at 4-8 ℃

Storage And Expiry Date
1. Liquid haemagglutination Antigens:store at 4-8 ℃ (don’t freeze) for 1 year.
2. Porcine Negitive Control –porcine serum: store at -15～-20℃ for 1 year.
3. Porcine Positive Control –porcine serum: store at -15～-20℃ for 1 year.
 

 

Porcine Pseudorabies Virus Indirect Haemagglutination AssayTest Kit

Price: € 20        (art.code:220-PSIHA)

 

Hog Cholera Indirect Haemagglutination Assay Test Kit

Price: € 20        (art.code:220-HIHA)

 

 

Porcine Circovirus Antibody ELISA Test Kit (PCV)

Price: € 303        (art.code:220-PCV-E)

 

 

Pseudorabies Virus gE Antibody Distinguishing Test Kit (PRV gE)Price: € 250        (art.code:220-PRV-gE)

 

 

Reagents provided

1. PRV-gE antigen-coated plates	2 pieces (96well)
2. Porcine Negetive Control-Porcine serum(green cap)	1 tube(1ml)
3. Porcine PositiveControl-Porcine serum (red cap)	1 tube(1ml)
4. Anti-pig IgG-HRP conjugate (yellow cap)	1 bottle(22ml)
5. Wash Concentrate (20xconcentrate) (white cap)	1 bottle(30ml)
6. Substrate A solution (red cap)	1 bottle(12ml)
7. Substrate B solution (black cap)	1 bottle(12ml)
8. Stop solution (blue cap)	1 bottle(12ml)
9. Sample Diluent (transparent cap)	1 bottle (50ml)

Specification: 96wells x 2
Expire : 6 months
Storage : 2-8 ℃, in dark place
 

 

Porcine Toxoplasmosis is on intimate terms with intensive culture ,toxoplasmosis is viruses to this disease, which generates much loss in the swine field, imperils femals and little swine once infected.

Discription and principles
Controlling this disease is based on method of serological diagnosis, test kit is enzyme immunassy for the detection antibodies in swine serum. Toxoplasmosis is coated on 96-well plate, put sample into microwell, antibodies react with toxoplasmosis-coated proteins, subsequent to a wash step, conjugate solution is added, unreacted conjugate is removed by washing, and a substrate is added to generated a bule color.

 

Reagents provided

1. Toxoplasmosis protein-coated plates	2 pieces( 96 wells)
2. Porcine Negetive Control-Porcine serum(green cap)	1 tube(2ml)
3. Porcine Positive Control-Porcine serum (red cap)	1 tube(2ml)
4. Conjugate solution (yellow cap)	1 bottle(22ml)
5. Wash Concentrate (20xconcentrate) (white cap)	1 bottle(30ml)
6. Substrate A solution (red cap)	1 bottle(12ml)
7. Substrate B solution (black cap)	1 bottle(12ml)
8. Stop solution (blue cap)	1 bottle(12ml)
9. Sample Diluent (transparent cap)	1 bottle (50ml)

Specification: 96wells x 2
Expire : 6 months
Storage : 2-8 ℃,in dark place

Swine Foot and Mouth Disease Distinguishing Test kit (FMD)

Price: € ?        (art.code:220-TIgG)

 

Swine foot and mouth is infectious disease which is an acute, thermal and high contact, caused by Picornaviridae, Rhinovirus of swine foot and mouth virus. It emerges different sizes blisters and ulcer in the hoof crown, heel, forks, vice- hoof ,skin, oral angle, cheek, tongue mucosa part, etc. Swine foot and mouth disease is of high incidence , fast infection, prevalent wide, which can easily lead to abortion, high dealth of little swine,and large economic loss.This disease is internationallly considered to be important prevention.

Intended use
Swine foot and mouth disease distinguishing test kit is frequently employed in the detection of anti-FMD non-structural 3ABC-protein antibodies in the serum and distinguishing naturally infected from vaccinated swine.
 

Discription and principles
Enzyme immunoassay for the detection of antibodies in swine serum to the 3ABC-antigen of swine foot and mouth disease. During the first incubation, diluted control-porcine serum and test serum are added, if specific 3ABC-antibodies present in the test serum, react with 3ABC-antigens on the microwell,subsequent to a wash step;then enzyme-conjugated antibody is added, it is specific to react antibody-antigens on the microwell, the unreacted conjugate is removed by washing, and a TMB substrate solution is added,.in the presence of enzyme,the substrate is converted to a blue product, then the HF solution is added to stop reaction,The absorbance at 630nm, OD value is measured by spectrophotometer for very microwell.

Reagents provided

1. FMD-3ABC protein-coated plates	2 pieces (96 wells)
2. Porcine Negetive Control-Porcine serum(green cap)	1 tube(1ml)
3. Porcine Positive Control-Porcine serum (red cap)	1 tube(1ml)
4. Anti-pig IgG-HRP conjugate (yellow cap)	1 bottle(22ml)
5. Wash Concentrate (20xconcentrate) (white cap)	1 bottle(30ml)
6. Substrate A solution (red cap)	1 bottle(12ml)
7. Substrate B solution (black cap)	1 bottle(12ml)
8. Stop solution (blue cap)	1 bottle(12ml)
9. Sample Diluent (transparent cap)	1 bottle (50ml)

 

Specification: 96wells x 2
Expire : 6 months
Storage : 2-8 ℃, in dark place
 

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