human IL-6
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human IL-6
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IMPORTANT : use cold 0.5M Acetic Acid instead of pure water in the reconstitution (the final pH of the protein solution should be 6.0) Recombinant Human Interleukin-6 (IL6) IL-6 (Human) 5.0 x 107 IU/mg 04-C009A 50μg 542 € 04-C009B 1mg 2.765 € Latest batch Expiry date 15/12/2009 Background: Cytokines of the IL6/GCSF/MGF family are glycoproteins of about 170 to 180 amino acid residues that contain four conserved cysteine residues involved in two disulphide bonds. They have a compact, globular fold (similar to other interleukins), stabilized by the 2 disulphide bonds. One half of the structure is dominated by a 4 alpha-helix bundle with a left-handed twist: the helices are anti-parallel, with 2 overhand connections, which fall into a 2-stranded anti-parallel beta-sheet. The fourth alpha helix is important to the biological activity of the molecule. Interleukin (IL)-6 is an important proinflammatory and immunoregulatory cytokine expressed by various cells. Interleukin-6 has been shown to inhibit the growth of early stage and to promote the proliferation of advanced stage melanoma cells in vitro. Description: Recombinant Human IL-6 produced in E. coli is a single, non-glycosylated polypeptidechain containing 185 amino acids and having a molecular mass of 21.0 kDa. Quality Control: Biological activity: rHuIL-6 is fully biologically active when compared to standard. TheED50 as determined by the dose-dependant stimulation of human TF-1 cells is less then 0.1 ng/ml, corresponding to a Specific Activity of 5.0 x 10 7 IU/mg.Purity: Greater than 98.0% as determined by:(a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. Molecular weight: 21 KD+/-10% determined by reduced SDS-PAGE.Amino-Acid Sequence: The sequence of the first five N-terminal amino acids wasdetermined and was found to be Ser-Pro-Val-Pro-Pro. Endotoxin: Less than 0.3ng/μg (0.3IEU/μg) determined by LAL test.Formulation: Lyophilized from a concentrated (0.5mg/ml) solution in water containing20mg/ml sodium acetic. Storage: Lyophilized rHuIL-6 although stable at room temperature for 3 weeks, should bestored desiccated below -18°C. Upon reconstitution rHuIL-6 should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. Reconstitution: It is recommended to reconstitute the lyophilized rHuIL-6 in 500mM aceticacid not less than 100μg/ml, which can then be further diluted to other aqueous solutions. |
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