Home Up Feedback Contents Search Sell your Prod. Meet us News           

Home • Up

rec human IFNg




+32 1658 9045


0032 (0)16 41 44 07

+32 1650 9045


Av. de l' Armée 68

B-1040 Brussels



tel 01 43 25 01 50

fax01 43 25 01 60

9, rue Lagrange

75005 Paris


tel 02 36 00 65 93

fax 02 36 00 65 94

20135 Milano


tel +32 1658 9045

fax +32 1650 9045


Tel 058 710 33 44

Fax 00 32 16 50 90 45

ul. Grunwaldzka 88A/2

81-771 Sopot


tel +81 78 386 0860

fax +81 78 306 0296


Chuo-ku, Kobe



Canada Montreal

Česká republika Praha


Finland Helsset

Ελλάς Αθήνα

Magyarország Budapest

Ireland Dublin



Norge Oslo

Polska Warszawa

Sverige Stockholm

Schweiz Züri

US New York

Other Countries
0032 (0)16 41 44 07






Recombinant Human Interferon-gamma (IFNr)
(Cat. No.: C027)

2 packages:


IFN gamma: 1MIU = 67 ug /  100 Euro

SJC03-02 Recombinant Human IFN-g 100μg/vial 195 Euro

    The major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced, among other things, by Interleukin-2, FGF-basic, and EGF.

    Recombinant Human Interferon gamma produced in E. coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 16879 Dalton.

Quality Control:
    Biological activity: The specific activity as determined in a viral resistance assay using VSV-WISH cells was found to be greater than 1.5 x 107 IU/ mg.
    Purity: Greater than 95% as determined by
              (a) Analysis by SEC-HPLC.
              (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
    Molecular weight: 16.8KD+/-10% determined by reduced SDS-PAGE.
    Isoelectric Point: the main zone between 8.1~9.1 analysis by IEF.
    Residual DNA: less than 300ng/mg analysis by solid phase blot.
    UV scan: the maximalimal absorption wave is 280+/-3nm.
    Amino-Acid Sequence: The sequence of the first fifteen N-terminal amino acids was determined and was found to be Gln-Asn-Pro-Tyr-Val-Lys-Glu-Ala-Glu-Asn-Leu-Lys-Lys-Tyr-Phe.
    Residual host cell protein: less than 0.1% analysis by ELISA.
    Endotoxin: Less than 1ng/µg (1IEU/µg) determined by LAL test.

Formulation: The protein was lyophilized after extensive dialysis from a concentrated (1mg/ml) solution in 10mM sodium Phosphate buffer pH=7.4+/-0.1. 

Storage: Lyophilized rHuIFN-gamma although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution rHuIFN-gamma should be stored at 4oC between 2-7 days and for future use below -18oC. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
    Please avoid freeze-thaw cycles. 

Reconstitution: It is recommended to reconstitute the lyophilized rHuPEG-GCSF in sterile 18M¦¸-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.


Copyright © 2002 GENTAUR Molecular Products
Last modified: 05/29/09