Sulfonamides Elisa
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Sulfonamides residues (Five) ELISA Test Kit Price: 361 art.code: 202-SULFR-E 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of Sulfamonomethoxine(SMM), Sulfadiazine(SD), Sulfamerazine(SM1), Sulfamethoxazole(SMZ) and Sulfathiazole(ST) in the tissue(chicken, pork), milk, honeyand egg. The conjugate antigen is pre-coated on the micro-well stripes. The Sulfonamides residues in the testing sample competes with the conjugate antigen pre-coated on the micro-well stripes, to interact with the antibody against Sulfonamides. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the testing sample has a negative correlation with the content of Sulfonamides residues in the sample. This value is compared to the standard curve and the content of the corresponding Sulfonamides residues is subsequently obtained.
2. Technical specifications Sensitivity : 1ppb Detection limit: Meat(chicken,pork), liver(chicken,pork),honey, egg 1ppb Serum, urine 4ppb Milk 20ppb Recovery rate Meat(chicken,pork), liver(chicken,pork) 70 ±10% Egg 65 ±10% Milk ,honey,serum 70 ±10% Cross-reaction rate: Sulfadiazine(SD) 100% Sulfamonomethoxine(SMM) 97.6% Sulfamerazine(SM1) 12% Sulfamethoxazole(SMZ) 63% Sulfathiazole(ST) 195%
This test kit is used to detect 4 Sulfonamides based on cross-reaction rate, its detection limit as follows: SD1ppb SMM 1ppb SMZ 2ppb ST 0.5ppb SM110ppb
96 wells /kit
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