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GENTAUR
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0032 (0)16 41 44 07
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0032 (0)16 41 44 07
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Total RNA Extraction Kits |
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The series of Total RNA Extraction Kits are specially designed for purification of total RNA from a variety of samples, such as bacteria, yeasts, plant tissues, animal tissues, cultured cells, fresh human whole blood, etc. The method uses detergents and a chaotropic salt to lyse cells and inactivate RNase, then RNA in chaotropic salt solutions binds to the glass fiber matrix in the columns. |
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After washing off the contaminants, purified RNA is eluted by RNase-free water. The entire procedure can be completed within 1 hour and the purified RNA is ready for plenty of applications. |
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Advantages |
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※ RT-PCR
※ cDNA synthesis
※ In vitro translation
※ Primer extension
※ Northern blotting
※ cDNA library construction |
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Storage Conditions |
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Store at room temperature (15 ~ 25°C) |
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Quality Control |
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The quality of Total RNA Extraction Kits is tested on a lot-to-lot basis. |
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Note |
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※ For research use only. Not for use in diagnostic or therapeutic procedures.
※ RB Buffer contains chaotropic salt which is a harmful and irritant agent. During operation, always wear a lab coat, disposable gloves, and protective goggles. |
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Use Conditions |
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※ Sample Source: animal tissue, whole blood/buffy coat, cultured animal cells, gram+/– bacteria
※ Sample Size: 30 mg for tissue, 107 animal cells, 300 μl of whole blood, 109 bacterial cells
※ Preparation Time: 20 min |
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References |
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Vogelstein, B., and Gillespie, D. (1979) Proc. Natl. Acad. Sci. USA 76: 615-619. |
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