Velocity

Features

High-speed, high fidelity DNA polymerase
High processivity
Fast amplification
Shorter PCR runs for longer templates
Robust, requires minimal optimization of the reaction

Applications

Cloning techniques where high fidelity is desirable
Blunt-end cloning
Amplification of difficult templates
Mutagenesis

Description

VELOCITY™ is a fast thermostable enzyme possessing 5’-3’ DNA polymerase and 3’-5’ proofreading exonuclease activities. VELOCITY provides high fidelity with an error-rate of 8 x 10-7 combined with a high speed DNA synthesis as fast as 15s/Kb for templates of up to 5Kb and 30s/Kb for templates longer than 5Kb. The inherent high speed DNA synthesis is due to the enhanced processivity of the polymerase.

VELOCITY is easy to use since it works with many different protocols and requires minimal optimization. The polymerase produces higher yields than most commercially available enzymes and generates blunt-ended amplicons. The error rate of VELOCITY DNA Polymerase (8 x 10-7) was determined using the rpsL fidelity assay (Lackovich et al., 2001; Fujii et al., 1999).

Two buffers are provided with VELOCITY DNA Polymerase. For general high fidelity applications, use 5x Hi Fi Reaction Buffer for GC-Rich or difficult templates, use 5x GC Reaction Buffer.

Unit Definition

One unit will incorporate 10nmoles of dNTPs in 30min at 72°C.

Concentration

2u/µl

Storage Conditions

VELOCITY DNA Polymerase can be stored for 12 months at -20°C.

Shipping Conditions

On Dry Ice or Blue Ice