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yT&A Cloning Kit |
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The yT&A cloning kit contain the yT&A vector and many reagents needed for ligation. It is a convenient pack for cloning PCR product generated using thermostable DNA polymerases, such as YEAtaq polymerase, which add a single terminal 3’-dA nucleotide overhang. After ligation, the mixture can be used directly to transform into competent cells (ECOSTM perform better) or be purified first to achieve higher transformation efficiency. The kit contains enough reagents for 20 reactions. |
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Features and Benefits |
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※ Fast!! Ligation can be completed within 5 ~ 20 minutes vs. 1 hour to overnight by using similar systems from other companies.
※ Higher transformation efficiency in comparison to other competitor’s cloning system.
※ More accurate results in comparison to other companies in colony pickings.
※ Two types of ligation buffer provided for your convenience. |
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Applications |
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※ Accepts terminal 3’-dA nucleotide overhang PCR products
※ Transform ligation product (purified/unpurified) into ECOSTM or competent cells
※ LacZ complementation for blue/white screening
※ Ampicillin marker for antibiotic selection |
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Storage Conditions |
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Store at –20°C |
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yT&A Cloning Vector(download cloning vector sequence) |
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※ With ampicillin resistance
※ With blue/white screening capability
※ All pass rigorous QC test to ensure the background colonies are close to zero
※ Very stable |
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Figure: Map and Sequence reference points of the yT&A cloning vector |
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Before the insert incorporated into the yT&A cloning vector, there is only 1 HindIII site and no BglII site. After the incorporation, the T and A nucleotides on the insert complement the sequence on the vector and generate these two new sites. This merit of yT&A vector makes cloning more economic and convenient. |
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Position of important sequences: |
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Multiple Cloning region |
434 to 490 |
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lac Z gene |
511 to 149 |
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Ampr gene |
2528 to 1671 |
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T7 promoter |
402 to 439 |
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M13 forward primer |
359 to 375 |
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M13 reverse primer |
528 to 507 |
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Restriction enzyme sites of yT&A cloning vector |
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Experimental Data |
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The result of ligation using the control insert DNA and reagents provided in yT&A cloning kit. |
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* The size of colony PCR on the agarose gel will change according to the primer design. In this colony PCR, the size of the band on the gel is |
+32 1658 9045
+32 1650 9045
