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HiYield Gel / PCR DNA Fragments
Extracting Kits |
HiYield Gel/PCR DNA Fragments Extraction Kits are designed to recover or concentrate DNA fragments from agarose gel, PCR or other enzymatic reactions. The method uses a chaotropic salt, guanidine thiocyanante to dissolve agarose gel and denature enzymes. DNA fragments in chaotropic salt solution bind to the glass fiber matrix of the spin column or in each well of the plate. |
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After washing off the contaminants, the purified DNA fragments are eluted by addition of low salt elution buffer or water. Salts, enzymes and unincorporated nucleotides are effectively removed from reaction mixtures without phenol extraction or alcohol precipitation. Typical recoveries are 60 ~ 80% for gel extraction and 80 ~ 90% for PCR cleanup. The entire procedure can be completed in 20 minutes and the eluted DNA is ready to use in reaction digestion, ligation, PCR, and sequencing reaction. |
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Applications |
※ DNA sequencing
※ DNA library screening and analysis
※ Restriction Enzyme Digestion
※ Ligation and transformation |
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Storage Conditions |
Store at room temperature (15 ~ 25°C) |
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Quality Control |
The quality of HiYield Gel/PCR DNA Fragments Extraction Kits is tested on a lot-to-lot basis. |
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Note |
※ For research use only. Not for use in diagnostic or therapeutic procedures.
※ Buffer contains guanidine thiocyanate which is a harmful and irritant agent. During operation, always wear a lab coat, disposable gloves, and a protective goggle. |
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Reference |
Vogelstein, B. and Gillespie, D. (1979) Proc. Natl. Acad. Sci. USA 76: 615-619. |
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