Last November, van der Gun et al.
published an article high-lighting the unique advantages of the
SAINT protein delivery reagent: SAINT-PhD.
1. Delivery of the protein is independent
size and charge.
2. Delivery efficiency is high: up to 98% of
adherent cells, up to 83% of suspension
cells and up to 70% of primary cells are
shown to have taken up the protein
transported by SAINT-PhD into the cells.
3. Intracellular delivery of proteins by SAINT-PhD
is not significantly affected by the presence
of serum. This
is a major advantage,
because most competitors (e.g. Bioporter)
are not able to deliver proteins into cells
in the presence of serum. Furthermore, the
presence of serum is self-evident in in vivo
protein delivery experiments and applications.
4. The delivered protein can exert its function
both in the cytoplasm and in the nucleus. For
example, a methyltransferase enzyme was
delivered using SAINT-PhD resulting in
DNA methylation leading to a decrease in
5. SAINT-PhD does not affect cell viability.
B.T.F. van der Gun et al., Serum
insensitive, intranuclear protein delivery by the multipurpose
cationic lipid SAINT-2, J. Control. Release. (2007),